| Project/Area Number |
21790773
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Kyushu University |
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Principal Investigator |
TANAKA Yoshihiro Kyushu University, 病院先端分子・細胞治療科, 学術研究員 (30536850)
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| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | GM-CSF / 樹状細胞 / センダイウイルスベクター / 肺癌 / センダイウィルスベクター |
|---|
| Research Abstract |
To identify the mechanism of antitumor effect induced by s.c. administration of GM-CSF gene transduced LLC (LLC/SeV/GM) cells, we conducted cDNA microarray analysis by using GM-CSF-sensitized DCs harvested from tumor-derived lymph nodes. The result showed that the genes involved in type I interferons-producing DCs (plasmacytoid DCs; pDCs), upon activation, were robustly upregulated in GM-DCs compared with its counterpart. Furthermore, the results of both in vivo depletion of pDCs and s.c. co-administration of TLR7 agonist with LLC/SeV/GM cells indicated that pDCs plays central roles on the antitumor effect induced by GM-CSF.
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