Identification of the important factor of GM-CSF sensitized dendritic cells that involves with the process of antitumor effect induced by GM-CSF
| Project/Area Number |
21790773
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Kyushu University |
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Principal Investigator |
TANAKA Yoshihiro Kyushu University, 病院先端分子・細胞治療科, 学術研究員 (30536850)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | GM-CSF / 樹状細胞 / センダイウイルスベクター / 肺癌 / センダイウィルスベクター |
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| Research Abstract |
To identify the mechanism of antitumor effect induced by s.c. administration of GM-CSF gene transduced LLC (LLC/SeV/GM) cells, we conducted cDNA microarray analysis by using GM-CSF-sensitized DCs harvested from tumor-derived lymph nodes. The result showed that the genes involved in type I interferons-producing DCs (plasmacytoid DCs; pDCs), upon activation, were robustly upregulated in GM-DCs compared with its counterpart. Furthermore, the results of both in vivo depletion of pDCs and s.c. co-administration of TLR7 agonist with LLC/SeV/GM cells indicated that pDCs plays central roles on the antitumor effect induced by GM-CSF.
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Report
(3 results)
Research Products
(3 results)
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[Presentation] Comparative microarray analysis implicates the pivotal participation of Type I Interferon related signaling pathway in antitumor effect provoked by in vivo GM-CSF gene transduced lung cancer cells2011
Author(s)
Megumi Narusawa, Hiroyuki Inoue, Yoshihiro Tanaka, Yosuke Yokota, Chika Sakamoto, Shohei Miyamoto, Tomoko Inoue, Makoto Inoue, Koichi Takayama, Mamoru Hasegawa, Yoichi Nakanishi, Kenzaburo Tani
Organizer
ASGCT 14th Annual Meeting
Place of Presentation
SEATTLE, WA.(採録決定済)
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