The role of Notch signaling in differentiation of human mast cells

• Project/Area Number: 21790905
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: University of Tsukuba
• Principal Investigator: SAKATA Mamiko University of Tsukuba, 大学院・人間総合科学研究科, 講師 (80451805)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: Notchシグナル / 臍帯血 / Delta1-Fc / Hes-1 / レトロウイルス / Notch / 肥満細胞 / ヒト臍帯血 / CD133陽性細胞 / 分化

Research Abstract

We tried to clarify the role of Notch signaling in the differentiation of human mast cells. Hematopoietic stem cells and progenitors, purified from human cord blood cells, were stimulated by plate-coated Notch ligands, or retroviral transduction of Hes-1. Notch - ligand stimulation did not induce mast cell differentiation. We succeeded in retroviral transduction of Notch signaling molecules, but the effect on differentiation of mast cells were still under the observation.

Research Products

• [Journal Article] 慢性骨髄性白血病とHes-1 (2011)
  • Author(s): 坂田(柳元)麻実子, 坂田麻実子
  • Journal Title: 血液フロンテイア 4月号 Pages: 62-66
• [Journal Article] Notch2 signaling is required for proper mast cell distribution and mucosal immunity in the intestine. (2011)
  • Author(s): Sakata-Yanagimoto, Sakai T, Miyake Y, Saito TI, Maruyama H, Morishita Y, Nakagami-Yamaguchi E, Kumano K, Yagita H, Fukayama M, Ogawa S, Kurokawa M, Yasutomo K, chiba S
  • Journal Title: Blood. 117(1) Pages: 128-134
  • Peer Reviewed
• [Journal Article] Notch2 signaling is required for proper mast cell distribution and mucosal immunity in the intestine. (2011)
  • Author(s): Mamiko Sakata-Yanagimoto, Toru Sakai Yasuyuki Miyake, Toshiki Saito, Haruhiko Maruyama, Yasuyuki Morishita, Etsuko Nakagami-Yamaguchi, Keiki Kumano, Yagita H, Masashi Fukayama, Seishi Ogawa, Mineo Kurokawa, Koji Yasutomo, Shigeru Chiba
  • Journal Title: Blood Volume: 117(1) Pages: 128-134
  • Peer Reviewed
• [Journal Article] 慢性骨髄性白血病とHesl (2011)
  • Author(s): 坂田-柳元麻実子, 千葉滋
  • Journal Title: 血液フロンティア Volume: 4月号(印刷中)
  • Peer Reviewed
• [Journal Article] Hes1 immortalizes committed progenitors and plays a role in blast crisis transition in chronic myelogenous leukemia. (2010)
  • Author(s): Nakahara F, Sakata-Yanagimoto M, Komeno Y, Kato N, Uchida T, Haraguchi K, Kumano K, Harada Y, Harada H, Kitaura J, Ogawa S, Kurokawa M, Kitamura T, Chiba S
  • Journal Title: Blood. 115(14) Pages: 2872-2881
  • Peer Reviewed
• [Journal Article] Hes1 immortalizes committed progenitors and plays a role in blast crisis transition in chronic myelogenous leukemia (2010)
  • Author(s): Nakahara F, Sakata-Yanagimoto M, Komeno Y, Kato N, Uchida T, Haraguchi K, Kumano K, Harada Y, Harada H, Kitaura J, Ogawa S, Karokawa M, Kitamura T, Chiba S.
  • Journal Title: Blood (in press)
  • Peer Reviewed
• [Journal Article] Gain-of-function of mutated C-CBL tumour suppressor in myeloid neoplasms. (2009)
  • Author(s): Sanada M, Suzuki T, Shih LY, Otsu M, Kato M, Yamazaki S, Tamura A, Honda H, Sakata-Yanagimoto M, Kumano K, Oda H, Yamagata T, Takita J, Gotoh N, Nakazaki K, Kawamata N, Onodera M, Nobuyoshi M, Hayashi Y, Harada H, Kurokawa M, Chiba S, Mori H, Ozawa K, Omine M, Hirai H, Nakauchi H, Koeffler HP, Ogawa S
  • Journal Title: Nature. 460(7257) Pages: 904-908
  • Peer Reviewed
• [Journal Article] Gain-of-function mutations and copy number increases of Notch2 in diffuse large B-cell lymphoma. (2009)
  • Author(s): Lee SY, Kumano K, Nakazaki K, Sanada M, Matsumoto A, Yamamoto G, Nannya Y, Suzuki R, Ota S, Ota Y, Izutsu K, Sakata-Yanagimoto M, Hangaishi A, Yagita H, Fukayama M, Seto M, Kurokawa M, Ogawa S, Chiba S
  • Journal Title: Cancer Sci. 100(5) Pages: 920-926
  • Peer Reviewed
• [Journal Article] Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL-15. (2009)
  • Author(s): Haraguchi K, Suzuki T, Koyama N, Kumano K, Nakahara F, Matsumoto A, Yokoyama Y, Sakata-Yanagimoto M, Masuda S, Takahashi T, Kamijo A, Takahashi K, Takanashi M, Okuyama Y, Yasutomo K, Sakano S, Yagita H, Kurokawa M, Ogawa S, Chiba S
  • Journal Title: Immunol. 182(10) Pages: 6168-6178
  • Peer Reviewed
• [Journal Article] Derivation of functional mature neutrophils from human embryonic stem cells. (2009)
  • Author(s): Yokoyama Y, Suzuki T, Sakata-Yanagimoto M, Kumano K, Higashi K, Takato T, Kurokawa M, Ogawa S, Chiba S
  • Journal Title: Blood. 113(26) Pages: 6584-6592
  • Peer Reviewed
• [Journal Article] Notch変異と分子標的療法 (2009)
  • Author(s): 坂田麻実子, 坂田麻実子
  • Journal Title: 血液・腫瘍科 58(1) Pages: 57-62
• [Journal Article] Gain-of-function of mutated C-CBL tumour suppressor in myeloid neoplasms (2009)
  • Author(s): Sanada M, Suzuki T, Shih LY, Otsu M, Kato M, Yamazaki S, Tamura A, Honda H, Sakata-Yanagimoto, M, Kumano K, Oda H, Yamagata T, Takita J, Gotoh N, Nakazaki K, Kawamata N, Onodera M, Nobuyoshi M, Hayashi Y, Harada H, Kurokawa M, Chiba S, Mori H, Ozawa K, Omine M, Hirai H, Nakauchi H, Koeffler HP, Ogawa S.
  • Journal Title: Nature 460(7257) Pages: 904-908
  • Peer Reviewed
• [Journal Article] Derivation of functional mature neutrophils from human embryonic stem cells (2009)
  • Author(s): Yokoyama Y, Suzuki T, Sakata-Yanagimoto M, Kumano K, Higashi K, Takato T, Kurokawa M, Ogawa S, Chiba S.
  • Journal Title: Blood 113(26) Pages: 6584-6592
  • Peer Reviewed
• [Presentation] Notch signaling as a mediator of myeloid differentiation and leukemic transformation (2010)
  • Author(s): 坂田-柳元麻実子
  • Organizer: 第33回分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Notch signaling as a mediator of myeloid differentiation and leukemic transformation (2010)
  • Author(s): 坂田-柳元麻実子
  • Organizer: 第5回Notch研究会
  • Place of Presentation: 野田
  • Year and Date: 2010-11-08
• [Presentation] Notchシグナルによる骨髄球の分化と白血病化 (2010)
  • Author(s): 坂田-柳元麻実子
  • Organizer: 第16回血液科学セミナー
  • Place of Presentation: 新宿
  • Year and Date: 2010-10-24
• [Presentation] レトロウイルスを用いたHes-1過剰発現により不死化した骨髄前駆細胞のマイクロアレイ解析 (2010)
  • Author(s): 三宅康行
  • Organizer: 第72回日本血液学会学術集会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-24
• [Presentation] Microarray analysis of myeloid progenitors transformed via retroviral transduction of Hes-1 (2010)
  • Author(s): Yasuyuki Miyake
  • Organizer: 第1回JSH国際シンポジウム
  • Place of Presentation: 秋田
• [Presentation] Microarray analysis of myeloid progenitors immortalized via retroviral transduction of Hes-1 (2010)
  • Author(s): Yasuyuki Miyake
  • Organizer: 第8回幹細胞シンポジウム
  • Place of Presentation: 淡路島
• [Presentation] レトロウイルスを用いたHes-1過剰発現により不死化した骨髄前駆細胞のマイクロアレイ解析 (2010)
  • Author(s): Yasuyuki Miyake, Mamiko Sakata-Yanaigmoto, Takayasu Kato, Hideharu Muto, Noriko Gotoh, Shigeru Chiba
  • Organizer: 第8回幹細胞シンポジウム
  • Place of Presentation: 淡路島
• [Presentation] Unique Gain-of-function of muatated c-CBL tumor suppressor in myeloid neoplasms (2009)
  • Author(s): Masashi Sanada
  • Organizer: The American Society of Hematology 51^<st> Annual Meeting
  • Place of Presentation: 米国New Orleans
  • Year and Date: 2009-12-06
• [Presentation] Hes1 confers self-renewal capability on committed progenitors and induces CML BC-like disease (2009)
  • Author(s): Fumio Nakahara
  • Organizer: 第71回日本血液学会学術集会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-10-24
• [Presentation] Gain-of-function mutations of c-Cbl tumor suppressor in MDS and MDS/MPD associated with 11qUPD (2009)
  • Author(s): Masashi Sanada
  • Organizer: 第71回日本血液学会学術集会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-10-24
• [Presentation] Gain-of-function mutations of c-Cb1 tumor suppressor in MDS and MDS/MPD associated with 11qUPD (2009)
  • Author(s): 真田昌
  • Organizer: 第71回日本血液学会学術集会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-10-24
• [Presentation] Deriviation of functional mature neutrophils from human embryonic stem cells (2009)
  • Author(s): Yasuhisa Yokoyama
  • Organizer: 第71回日本血液学会学術集会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-10-23
• [Presentation] Gain-of-function mutations of c-Cbl tumor suppressor associated with 11qUPD in myeloid neoplasms (2009)
  • Author(s): Masashi Sanada
  • Organizer: 第68回日本癌学会学術集会総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-02
• [Presentation] Gain-of-function mutations of c-Cb1 tumor suppressor associated with 11qUPD in myeloid neoplasms (2009)
  • Author(s): 真田昌
  • Organizer: 第68回日本癌学会学術集会総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-02
• [Presentation] Hes1 confers self-renewal capability on committed progenitors and induces CML BC-like disease (2009)
  • Author(s): Fumio Nakahara
  • Organizer: 第68回日本癌学会学術集会総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-01