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Structural property of soybean protein P34 and specific IgE response to recombinant P34 in patients with soybean allergy

Research Project

Project/Area Number 21790980
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionGifu University

Principal Investigator

MORITA Hideyuki  岐阜大学, 医学系研究科, 非常勤講師 (50444302)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords大豆アレルギー / リコンビナントP34 / エンドトキシン / エピトープ / リンパ球幼若化反応 / Immuno CAP / ImmunoCAP / IgE western blot / CAP-RAST / リコンビナントタンパク / 大豆主要アレルゲンP34
Research Abstract

Our new purification method did not change the immune responses to rP34. PBMC_s from allergic subjects, but not from control subjects, showed an enhanced allergen-stimulated proliferation response to endotoxin-free recombinant P34. Furthermore, the ImmunoCAP system using our purified recombinant P34 that retains its tertiary structure may be useful for the identification of patients with soybean allergy or for the indication of outgrown allergy. Purified recombinant P34 may be useful tool for the evaluation of soybean allergies, and a specific immunotherapy for soybean allergy based on recombinant should be considered.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (16 results)

All 2012 2011 2009

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (11 results)

  • [Journal Article] Structural property of soybean protein P34 and specific IgE response to recombinant P34 in patients with soybean allergy2012

    • Author(s)
      H. Morita, H. Kaneko, H. Ohnishi, Z. Kato, K. Kubota, T. Yamamoto, E. Matsui, T. Teramoto, T. Fukao, K. Kasahara and N. Kondo
    • Journal Title

      International Journal of Molecular Medicine

      Volume: 29 Pages: 153-158

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural property of soybean protein P34 and specific IgE response to recombinant P34 in patients with soybean allergy2012

    • Author(s)
      Hideyuki Morita
    • Journal Title

      International journal of molecular medicine

      Volume: 29 Pages: 153-158

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antigen-specific immune response to endotoxin-free recombinant P342011

    • Author(s)
      H. Morita, H. Kaneko, H. Ohnishi, Z. Kato and N. Kondo
    • Journal Title

      Allergy

      Volume: 66 Pages: 985-986

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antigen-specific immune response to endotoxin-free recombinant P342011

    • Author(s)
      森田秀行
    • Journal Title

      Allergy

      Volume: (In Press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 食物アレルギー寛容誘導に向けた抗原エピトープ解析と治療戦略2009

    • Author(s)
      森田秀行
    • Journal Title

      Dermatology View 8

      Pages: 966-969

    • Related Report
      2009 Annual Research Report
  • [Presentation] 大豆主要アレルゲンRecombinant P34を用いた免疫学的手法の臨床応用2011

    • Author(s)
      森田秀行
    • Organizer
      第61回日本アレルギー学会秋季学術大会
    • Place of Presentation
      東京
    • Year and Date
      2011-11-10
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Recombinant P34を用いたImmunoCAP Systemの有用性の検討2011

    • Author(s)
      森田秀行
    • Organizer
      第48回日本小児アレルギー学会
    • Place of Presentation
      福岡
    • Year and Date
      2011-10-23
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Recombinant P34を用いたImmnoCAP Systemの有用性の検討2011

    • Author(s)
      森田秀行
    • Organizer
      第6回岐阜小児アレルギー学会
    • Place of Presentation
      岐阜
    • Year and Date
      2011-06-03
    • Related Report
      2011 Final Research Report
  • [Presentation] Recombinant P34を用いたImmunoCAP Systemの有用性の検討2011

    • Author(s)
      森田秀行
    • Organizer
      第6回岐阜小児アレルギー学会
    • Place of Presentation
      岐阜
    • Year and Date
      2011-06-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] 食物アレルギーにおける抗原特異的リンパ球幼若化反応の意義と応用2009

    • Author(s)
      森田秀行
    • Organizer
      第46回日本小児アレルギー学会
    • Place of Presentation
      福岡
    • Year and Date
      2009-12-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] 食物アレルギーにおける抗原特異的リンパ球幼若化反応の意義と応用2009

    • Author(s)
      森田秀行
    • Organizer
      第46回日本小児アレルギー学会学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2009-12-05
    • Related Report
      2011 Final Research Report
  • [Presentation] B and T cell epitope analysis in soybean allergic patients-dentification of T cell epitope by in silicosystem2009

    • Author(s)
      森田秀行
    • Organizer
      第19回国際喘息学会日本・北アジア部会
    • Place of Presentation
      東京
    • Year and Date
      2009-07-10
    • Related Report
      2011 Final Research Report
  • [Presentation] 大豆アレルギー患者のHLAおよびin silicoの手法を用いたT細胞反応性エピトープの解析2009

    • Author(s)
      森田秀行
    • Organizer
      第19回国際喘息学会日本北アジア部会
    • Place of Presentation
      東京
    • Year and Date
      2009-07-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] 母乳栄養とアレルギー疾患発症との関連について2009

    • Author(s)
      森田秀行
    • Organizer
      第21回日本アレルギー学会春季臨床大会
    • Place of Presentation
      岐阜
    • Year and Date
      2009-06-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] 大豆主要アレルゲンP34を用いた大豆アレルギー患者の免疫学的検討2009

    • Author(s)
      森田秀行
    • Organizer
      第21回日本アレルギー学会春期学術大会
    • Place of Presentation
      岐阜
    • Year and Date
      2009-06-04
    • Related Report
      2011 Final Research Report
  • [Presentation] 免疫寛容誘導を目的とした大豆P34分解ペプチドのBおよびTリンパ球反応性の評価2009

    • Author(s)
      森田秀行
    • Organizer
      代112回日本小児科学会学術集会
    • Place of Presentation
      奈良
    • Year and Date
      2009-04-19
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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