The analysis of the genetic alteration in Sertoli cells when the spermatogenesis occurred.

• Project/Area Number: 21791515
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Nagoya City University
• Principal Investigator: UMEMOTO Yukihiro Nagoya City University, 大学院・医学研究科, 講師 (80381812)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: mRNA / clusterin / protamine2 / WNT5A / 造精機能 / sertoli cell / leydig cell / 停留精巣

Research Abstract

As a rat model of spermatogenesis dysfunction, we developed a cryptorchidism model rat and have reported this model rat. The quantity of Clusterin did not differ between the testes at all periods, while WNT5A were significantly decreased in the undescended testis compared to the normal testis at all periods. Immunohistological staining of Clusterin and WNT5A revealed no difference between the normal and the undescended testes at all periods.

Research Products

• [Journal Article] Cyclooxygenase-2 protects germ cells against spermatogenesis disturbance in experimental cryptorchidism model mice. (2011)
  • Author(s): Kubota H, Sasaki S, Kubota Y, Umemoto Y, Yanai Y, Tozawa K, Hayashi Y, Kohri K.
  • Journal Title: Journal of Andrology 32 Pages: 77-85
  • Peer Reviewed
• [Journal Article] Cyclooxygenase-2 protects germ cells against spermatogenesis disturbance in experimental cryptorchidism model mice. (2011)
  • Author(s): Kubota H, Umemoto Y, et al.
  • Journal Title: Journal of Andrology Volume: 32 Pages: 77-85
  • Peer Reviewed
• [Journal Article] Azoospermia patient with quadruplicate DAZ genes. (2009)
  • Author(s): Umemoto Y, Sasaki S, Kohri K.
  • Journal Title: Int J Urol 16 Pages: 768-768
  • NAID: 10025196538
  • Peer Reviewed
• [Journal Article] Azoospermia patient with quadruplicate DAZ genes. (2009)
  • Author(s): Umemoto Y, Sasaki S
  • Journal Title: Int J Urol VOL.16 Pages: 768-768
  • NAID: 10025196538
  • Peer Reviewed
• [Presentation] 造精機能に関与するmRNAの検討について (2010)
  • Author(s): 梅本幸裕、佐々木昌一、岩月正一郎、小島祥敬、水野健太郎、神谷浩行、矢内良昌、窪田裕樹、窪田泰江、池内隆人、林祐太郎、郡健二郎
  • Organizer: 第98回日本泌尿器科学会総会
  • Place of Presentation: 岩手市
  • Year and Date: 2010-04-29
• [Presentation] 造精機能に関与するmRNAの検討について (2010)
  • Author(s): 梅本幸裕、佐々木昌一, 他
  • Organizer: 第98回日本泌尿器科学会総会
  • Place of Presentation: 岩手県盛岡市・いわて県民情報交流センター「アイーナ」
  • Year and Date: 2010-04-29
• [Presentation] WNT5A is the mRNA carried by sperm, and may take part in spermatogenesis. (2010)
  • Author(s): Umemoto Y, Sasaki S, etc.
  • Organizer: The 4^<th> Greatwall International Andrology Forum
  • Place of Presentation: 中国(深セン)
  • Year and Date: 2010-03-19
• [Presentation] WNT5A is the mRNA carried by sperm, and may take part in spermatogenesis. (2010)
  • Author(s): Umemoto Y, Sasaki S, Iwatuki S, Mizuno K, Kojima Y, Hayashi Y, Kohri K
  • Organizer: The 4^<th> Greatwall International Andrology Forum
  • Place of Presentation: Shenzhen (China)
  • Year and Date: 2010-03-01