Two pore-domain potassium channel in retina : its functional expression and role of neuroprotection.

Research Project

Project/Area Number	21791674
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Ophthalmology
Research Institution	Kanazawa University
Principal Investigator	SAKURADA Norimasa Kanazawa University, 医学系, 協力研究員 (80377390)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords	Kチャンネル / 網膜 / 神経保護 / ぶどう膜
Research Abstract	The mRNA of two pore-domain potassium channel (K(2P)) was detected, and immunohistochemical findings showed TRAAK and TREK-2 expressions in the rat retina. However, patch-clamp study failed to detect K(2P) currents in isolated retinal cells. A KCNQ channel current was identified in porcine ciliary epithelial cells. Molecular biological analysis revealed that mRNAs of KCNQ1, 4 and 5 were expressed dominantly in ciliary body.