Analysis of protein ubiquitination for age related macular degeneration.

Research Project

Project/Area Number	21791728
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Ophthalmology
Research Institution	独立行政法人国立病院機構東京医療センター・(臨床研究センター) (2010) 独立行政法人国立病院機構(東京医療センター臨床研究センター) (2009)
Principal Investigator	OKAMOTO Haru 独立行政法人国立病院機構東京医療センター・(臨床研究センター), 分子細胞生物学研究部, 研究員 (40374160)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	老化 / ストレス / 蛋白質 / プロテオーム / 細胞・組織
Research Abstract	To examine the protein ubiquitination for age related macular degeneration, analysis of phagocytosis using ARPE-19 cells and proteomic analysis of plasma sample from patients with AMD were performed. These analyses reveal the following results 1) Phagocytosis of photoreceptor outer segment with oxidized lipids affects ubiquitin-proteasome system in RPE cells. 2) Peptides from ubiquitin/ubiquitinated proteins were detected in plasma samples from patients with AMD. But detection of ubiquitin was not specific for AMD.