Mechanism regulating sex determination of the germline progenitors in Drosophila

• Project/Area Number: 21870054
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設) (2010); National Institutes of Natural Sciences Okazaki Research Facilities (2009)
• Principal Investigator: HASHIYAMA Kazuya 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 特別協力研究員 (20546949)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000); Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000); Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
• Keywords: ショウジョウバエ / 生殖細胞 / 性決定 / 配偶子形成

Research Abstract

Germline sex is imposed by surrounding soma after gonad formation. Here, we found that Sex lethal (Sxl) gene is expressed in the migrating PGCs in a female (XX) specific manner. To examine whether Sxl is able to induce female fate in the male (XY) PGCs, we expressed Sxl in XY PGCs. Then we transplanted the XY PGCs into normal female embryos and examined their fate. The feminized germline contributed progeny production. This suggests that Sxl is both required and sufficient for the female fate in the germline.

Research Products

• [Presentation] Sex lethal acts autonomously in the primordial germ cells to initiate female fate in Drosophila. (2011)
  • Author(s): Kazuya Hashiyama, Yoshiki Hayashi, Satoru Kobayashi.
  • Organizer: IRB-Barcelona, Barcelona, Spain.
  • Place of Presentation: Invited seminar
  • Year and Date: 2011-04-21
• [Presentation] Sex lethal acts autonomously in the primordial germ cells to initiate female fate in Drosophila. (2011)
  • Author(s): Kazuya Hashiyama, Yoshiki Hayashi, Satoru Kobayashi.
  • Organizer: Heinrich-Heine Universitat, Dusseldorf, Germany.
  • Place of Presentation: Invited seminar
  • Year and Date: 2011-03-28
• [Presentation] Sex lethal acts autonomously in the primordial germ cells to initiate female fate in Drosophila. (2011)
  • Author(s): Kazuya Hashiyama, Yoshiki Hayashi, Satoru Kobayashi.
  • Organizer: JSDB/Gfe Joint meeting, Dresden, Germany.
  • Place of Presentation: Oral presentation
  • Year and Date: 2011-03-24
• [Presentation] Sex lethal acts autonomously in the primordial germ cells to initiate female fate in Drosophila (2011)
  • Author(s): Kazuya Hashiyama
  • Organizer: JSDB/Gfe Joint Meeting
  • Place of Presentation: Dresden(Germany)
  • Year and Date: 2011-03-24
• [Presentation] Sex lethal acts autonomously in the germline progenitors to initiate female development in Drosophila. (2010)
  • Author(s): Kazuya Hashiyama, Yoshiki Hayashi, Satoru Kobayashi.
  • Organizer: Carnegie institution, Baltimore, U.S.A.
  • Place of Presentation: Oral presentation
  • Year and Date: 2010-10-14
• [Presentation] Sex lethal acts autonomously in the germline progenitors to initiate female development in Drosophila. (2010)
  • Author(s): Kazuya Hashiyama, Yoshiki Hayashi, Satoru Kobayashi.
  • Organizer: Cold Spring Harbor Laboratory Meeting, New York, U.S.A.
  • Place of Presentation: Poster presentation
  • Year and Date: 2010-10-06
• [Presentation] Sex lethal acts autonomously in the germline progenitors to initiate female development in Drosophila (2010)
  • Author(s): Kazuya Hashiyama
  • Organizer: Cold Spring Harbor Laboratory Meeting
  • Place of Presentation: New York(USA)
  • Year and Date: 2010-10-06
• [Presentation] Mechanism regulating sex determination of the germline progenitors in Drosophila. (2010)
  • Author(s): Kazuya Hashiyama, Yoshiki Hayashi, Satoru Kobayashi.
  • Organizer: Sattellite Symopium to SDB and JSDB joint meeting, Albuqurque, U.S.A.
  • Place of Presentation: Oral presentation
  • Year and Date: 2010-08-05
• [Presentation] Mechanism regulating sex determination of the germline progenitors in Drosophila (2010)
  • Author(s): Kazuya Hashiyama
  • Organizer: Satellite Symposium to SDB and JSDB joint meeting
  • Place of Presentation: Albuquerque(USA)
  • Year and Date: 2010-08-05
• [Presentation] ショウジョウバエ始原生殖細胞における自律的性決定機構 (2009)
  • Author(s): 橋山一哉
  • Organizer: 日本動物学会
  • Place of Presentation: 静岡グランシップ(静岡県)
  • Year and Date: 2009-09-19
• [Presentation] ショウジョウバエ始原生殖細胞における自律的性決定機構 (2009)
  • Author(s): 橋山一哉
  • Organizer: 木曽生物学セミナー
  • Place of Presentation: 桑谷山荘(愛知県)
  • Year and Date: 2009-08-29
• [Presentation] Germline-autonomous mechanism leading to sexual dimorphism in Drosophila primordial germ cells (2009)
  • Author(s): 橋山一哉
  • Organizer: 日本発生生物学会
  • Place of Presentation: 朱鷺メッセ(新潟県)
  • Year and Date: 2009-05-30