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Research on mechanism of a novel signal polypeptide-induced innate immune activation.

Research Project

Project/Area Number 21890125
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionNational Institute of Biomedical Innovation (2010)
Osaka University (2009)

Principal Investigator

KOBIYAMA Kouji  National Institute of Biomedical Innovation, 研究員 (50547700)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000)
Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000)
Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
Keywordsアジュバント / I型IFN / 自然免疫 / N'-CARD-PTD / NDH / 新規アジュバント開発
Research Abstract

The aim of this research is to clarify mechanism of a novel signal polypeptide (N'-CARD-PTD)-induced innate immune activation and adjuvanticity of N'-CARD-PTD. Previous research we identified histone H2B is involved in dsDNA-mediated innate immune activation. Recently we synthesized the newly fusion protein, N'-CARD (nuclear localization signal of H2B fused CARD of IPS-1), activated nuclear DNA helicase II (NDH)-mediated innate immune responses. In this research, we clarify that protein transduction domain fused N'-CARD (N'-CARD-PTD) activated dendritic cells depend on type I IFN, but not TLRs. As a vaccine adjuvant, N'-CARD-PTD strongly induced antigen specific antibody response against various antigen.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (20 results)

All 2011 2010 2009 2008 Other

All Journal Article (11 results) (of which Peer Reviewed: 8 results) Presentation (2 results) Book (3 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] NLRP4 negatively regulates autophagic processes through an association with Beclin1.2011

    • Author(s)
      Jounai N, Kobiyama K, Shiina M, Ogata K, Ishii KJ, Takeshita F.
    • Journal Title

      J.Immunol. 186

      Pages: 1646-1655

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] NLRP4 negatively regulates autophagic processes through an association with Beclin1.2011

    • Author(s)
      Jounai N, Kobiyama K, Shiina M, Ogata K, Ishii KJ, Takeshita F.
    • Journal Title

      Journal of Immunology

      Volume: 186 Pages: 1646-1655

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of intracellular signaling using protein transduction technology.(Review)2010

    • Author(s)
      Kobiyama K, Jounai N, Ishii KJ, Horii T, Suzuki K, Ryo A, Takeshita F.
    • Journal Title

      Crit Rev Immunol. 30

      Pages: 395-421

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasmacytoid dendritic cells delineate immunogenicity of influenza vaccine subtypes.2010

    • Author(s)
      Koyama S, Aoshi T, Tanimoto T, Kumagai Y, Kobiyama K, Tougan T, Sakurai K, Coban C, Horii T, Akira S, Ishii KJ.
    • Journal Title

      Sci Transl Med. 2

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Extra-chromosomal histone H2B mediates innate antiviral immune responses induced by intracellular double-stranded DNA.2010

    • Author(s)
      Kobiyama K, Takeshita F, Jounai N, Sakaue-Sawano A, Miyawaki A, Ishii KJ, Kawai T, Sasaki S, Hirano H, Ishii N, Okuda K, Suzuki K.
    • Journal Title

      J.Virol. 84

      Pages: 822-832

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of intracellular signaling using protein transduction technology.2010

    • Author(s)
      Kobiyama K, Jounai N, Ishii KJ, Horii T, Suzuki K, Ryo A, Takeshita F.
    • Journal Title

      Critical Reviews in Immunology

      Volume: 30 Pages: 395-421

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasmacytoid dendritic cells delineate immunogenicity of influenza vaccine subtypes.2010

    • Author(s)
      Koyama S, Aoshi T, Tanimoto T, Kumagai Y, Kobiyama K, Tougan T, Sakurai K, Coban C, Horii T, Akira S, Ishii KJ.
    • Journal Title

      Science Translational Medicine

      Volume: 2 Pages: 24-25

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNAセンサーとその生理的意義2010

    • Author(s)
      小檜山康司、石井健
    • Journal Title

      細胞工学

      Volume: 29 Pages: 1004-1013

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 自然免疫とワクチン開発2010

    • Author(s)
      小檜山康司、石井健
    • Journal Title

      医学のあゆみ

      Volume: 234 Pages: 608-614

    • Related Report
      2010 Annual Research Report
  • [Journal Article] アジュバントに関する最新の話題2010

    • Author(s)
      小檜山康司、石井健
    • Journal Title

      臨床と微生物

      Volume: 37 Pages: 3-10

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Extra-chromosomal histone H2B mediates innate antiviral immune responses induced by intracellular double-stranded DNA.2010

    • Author(s)
      Kobiyama K, Takeshita F, Jounai N, Sakaue-Sawano A, Miyawaki A, Ishii KJ, Kawai T, Sasaki S, Hirano H, Ishii N, Okuda K, Suzuki K.
    • Journal Title

      Journal of virology 84

      Pages: 822-832

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Extra-chromosomal histone H2B mediates innate antiviral immune responses induced by intracellular double-stranded DNA.2010

    • Author(s)
      Kobiyama K, Takeshita F, Jounai N, Suzuki K, Ishii KJ.
    • Organizer
      14th International Congress of Immunology.
    • Place of Presentation
      Kobe, Japan.
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Nuclear distribution of IPS-1 CARD activates distinct innate immune signaling to augment vaccine immunogenicity.2009

    • Author(s)
      Kobiyama K, Ishii KJ, Takeshita F.
    • Organizer
      Keystone Symposia on Molecular and Cellualr Biology.
    • Place of Presentation
      Fairmont Banff Springs, Banff, Alberta, Canada.
    • Year and Date
      2009-04-01
    • Related Report
      2009 Annual Research Report
  • [Book] DNAセンサーとその生理的意義(Vol.29 No.10)2010

    • Author(s)
      小檜山康司、石井健
    • Publisher
      細胞工学
    • Related Report
      2010 Final Research Report
  • [Book] 自然免疫とワクチン開発(Vol.234 No.5)2010

    • Author(s)
      小檜山康司、石井健
    • Publisher
      医学のあゆみ
    • Related Report
      2010 Final Research Report
  • [Book] アジュバントに関する最新の話題(37巻)2010

    • Author(s)
      小檜山康司、石井健
    • Publisher
      臨床と微生物
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 染色体外ヒストンH2Bを介する二本鎖DNA認識機構の利用2011

    • Inventor(s)
      武下文彦、小檜山康司、吉田篤司
    • Industrial Property Rights Holder
      公立大学法人横浜市立大学
    • Patent Publication Number
      2011-109982
    • Acquisition Date
      2011-06-09
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] カスパーゼリクルートメントドメインを応用した新規リコンビナントタンパク及びその用途2010

    • Inventor(s)
      武下文彦、小檜山康司、奥田研爾
    • Industrial Property Rights Holder
      公立大学法人横浜市立大学
    • Acquisition Date
      2010-03-04
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] カスパーゼリクルートメントドメインを応用した新規リコンビナントタンパク及びその用途2008

    • Inventor(s)
      武下文彦、小檜山康司、奥出研爾
    • Industrial Property Rights Holder
      横浜市立大学
    • Filing Date
      2008-08-29
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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