Research on mechanism of a novel signal polypeptide-induced innate immune activation.
| Project/Area Number |
21890125
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | National Institute of Biomedical Innovation (2010)
Osaka University (2009) |
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Principal Investigator |
KOBIYAMA Kouji National Institute of Biomedical Innovation, 研究員 (50547700)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000)
Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000)
Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
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| Keywords | アジュバント / I型IFN / 自然免疫 / N'-CARD-PTD / NDH / 新規アジュバント開発 |
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| Research Abstract |
The aim of this research is to clarify mechanism of a novel signal polypeptide (N'-CARD-PTD)-induced innate immune activation and adjuvanticity of N'-CARD-PTD. Previous research we identified histone H2B is involved in dsDNA-mediated innate immune activation. Recently we synthesized the newly fusion protein, N'-CARD (nuclear localization signal of H2B fused CARD of IPS-1), activated nuclear DNA helicase II (NDH)-mediated innate immune responses. In this research, we clarify that protein transduction domain fused N'-CARD (N'-CARD-PTD) activated dendritic cells depend on type I IFN, but not TLRs. As a vaccine adjuvant, N'-CARD-PTD strongly induced antigen specific antibody response against various antigen.
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Report
(3 results)
Research Products
(20 results)
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[Journal Article] Plasmacytoid dendritic cells delineate immunogenicity of influenza vaccine subtypes.2010
Author(s)
Koyama S, Aoshi T, Tanimoto T, Kumagai Y, Kobiyama K, Tougan T, Sakurai K, Coban C, Horii T, Akira S, Ishii KJ.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Extra-chromosomal histone H2B mediates innate antiviral immune responses induced by intracellular double-stranded DNA.2010
Author(s)
Kobiyama K, Takeshita F, Jounai N, Sakaue-Sawano A, Miyawaki A, Ishii KJ, Kawai T, Sasaki S, Hirano H, Ishii N, Okuda K, Suzuki K.
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Journal Title
J.Virol. 84
Pages: 822-832
Related Report
Peer Reviewed
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[Journal Article] Plasmacytoid dendritic cells delineate immunogenicity of influenza vaccine subtypes.2010
Author(s)
Koyama S, Aoshi T, Tanimoto T, Kumagai Y, Kobiyama K, Tougan T, Sakurai K, Coban C, Horii T, Akira S, Ishii KJ.
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Journal Title
Science Translational Medicine
Volume: 2
Pages: 24-25
Related Report
Peer Reviewed
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[Journal Article] Extra-chromosomal histone H2B mediates innate antiviral immune responses induced by intracellular double-stranded DNA.2010
Author(s)
Kobiyama K, Takeshita F, Jounai N, Sakaue-Sawano A, Miyawaki A, Ishii KJ, Kawai T, Sasaki S, Hirano H, Ishii N, Okuda K, Suzuki K.
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Journal Title
Journal of virology 84
Pages: 822-832
Related Report
Peer Reviewed
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