| Project/Area Number |
21890125
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Virology
|
|---|
| Research Institution | National Institute of Biomedical Innovation (2010)
Osaka University (2009) |
|---|
Principal Investigator |
KOBIYAMA Kouji National Institute of Biomedical Innovation, 研究員 (50547700)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000)
Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000)
Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
|
|---|
| Keywords | アジュバント / I型IFN / 自然免疫 / N'-CARD-PTD / NDH / 新規アジュバント開発 |
|---|
| Research Abstract |
The aim of this research is to clarify mechanism of a novel signal polypeptide (N'-CARD-PTD)-induced innate immune activation and adjuvanticity of N'-CARD-PTD. Previous research we identified histone H2B is involved in dsDNA-mediated innate immune activation. Recently we synthesized the newly fusion protein, N'-CARD (nuclear localization signal of H2B fused CARD of IPS-1), activated nuclear DNA helicase II (NDH)-mediated innate immune responses. In this research, we clarify that protein transduction domain fused N'-CARD (N'-CARD-PTD) activated dendritic cells depend on type I IFN, but not TLRs. As a vaccine adjuvant, N'-CARD-PTD strongly induced antigen specific antibody response against various antigen.
|
