Fundamental research project to elucidate the process in which bone marrow side population (SP) cells differentiate into muscle fibers
Project/Area Number |
21890259
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Morphological basic dentistry
|
Research Institution | Tokyo Dental College |
Principal Investigator |
IWANUMA Osamu Tokyo Dental College, 歯学部, 助教 (80506866)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥2,275,000 (Direct Cost: ¥1,750,000、Indirect Cost: ¥525,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,365,000 (Direct Cost: ¥1,050,000、Indirect Cost: ¥315,000)
|
Keywords | 骨髄SP細胞 / 筋線維 / 筋分化 / FACS / 成長因子 / 筋特異的転写調節因子 / 分化 / TGF-β1 / 免疫染色 / 筋芽細胞 / 共培養 / 筋管様細胞 |
Research Abstract |
The decrease in myofunction is a well known degenerative change with age. To elucidate the mechanisms of myofunctional reactivation, we investigated specific proteins that promote the differentiation of bone marrow side population cells, which influence muscle development. Bone marrow side population cells were cultivated in low serum media, to which various combinations of growth factors were added. As factors involved in myofunctional reactivation, TGF-β1 and IGF-1 influenced the early and subsequent stages of differentiation, respectively.
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Report
(3 results)
Research Products
(11 results)