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Elucidation of Maturation Control Mechanisms in Grape Fruits by Metabolomics and Transcriptomics

Research Project

Project/Area Number 21K05578
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionIshikawa Prefectural University

Principal Investigator

TAKAI Mei  石川県立大学, 生物資源環境学部, 准教授 (70589770)

Co-Investigator(Kenkyū-buntansha) 及川 彰  京都大学, 農学研究科, 教授 (50442934)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords果実成熟 / 植物ホルモン / アブシジン酸 / 遺伝子発現解析 / メタボローム解析 / 環状剥皮 / CE-MSメタボローム解析 / RNA-Seq / アントシアニン生合成関連遺伝子 / ABA代謝関連遺伝子 / ブドウ果実の成熟
Outline of Research at the Start

果実は成熟過程において活発な生理変化に伴い、品質(味、色、香り、栄養素等)に直接関連する代謝産物の蓄積と同時に生理活性物質の変化も大きい。非クライマクテリック型果実であるブドウの成熟制御機構はまた解明されていない。本研究では、メタボローム解析とトランスクリプトーム解析等手法により成熟表現型の異なる果実を比較し、成熟誘導および進行の制御因子の探索を目的とする。得られた知見は、ブドウの成熟・着色を人為的に制御するための技術開発や環境適応型品種の育種に応用できるだけでなく、植物生理学などの基礎生物学の発展にも貢献すると考えられる。

Outline of Final Research Achievements

To elucidate the maturation control mechanisms in non-climacteric grape fruits, we performed metabolome analysis of metabolites (especially plant hormones) and transcriptome analysis (RNA-Seq) of comprehensive gene expression using fruits with different maturation phenotypes. The results suggested that there is crosstalk between various plant hormones during the grape fruit maturation process, and abscisic acid (ABA) plays an important role in maturation. The progression patterns of coloration varied greatly between varieties, were related to fluctuations in endogenous ABA content, and these traits were suggested to be heritable. Additionally, it was suggested that at least part of the ABA in the fruits may have been translocated from other tissues.

Academic Significance and Societal Importance of the Research Achievements

果実の成熟はエチレンの制御による追熟型(クライマクテリック型)とエチレンが生成しない非追熟型(非クライマクテリック型)に大別される。ブドウのような非クライマクテリック型果実の成熟のメカニズムはまた解明されていない。本研究では、各種植物ホルモン(特にアブシジン酸(ABA)がブドウ果実成熟過程における機作を検討することにより、非クライマクテリック型果実の成熟制御機構の解明に知見を提供できる。一方、ブドウ果皮の色は市場価格を影響する重要な品質であり、本研究の成果は地球温暖化等による着色不良問題の解決に理論的および実用的な技術開発に役に立つと考えられる。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (7 results)

All 2024 2023 2022 2021

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Transcriptome analysis for ‘Ruby-Roman’ grapes grafted on diploid and tetraploid rootstocks2024

    • Author(s)
      Gao-Takai M., Li T.S., Lin Z., Segawa T., Takagi H.,Katayama-Ikegami A.
    • Journal Title

      Acta Horticulturae

      Volume: -

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of Grapevine Rootstocks on Anthocyanin Biosynthesis, Sugar Contents, and Endogenous Hormone Contents During the Berry Maturation of ‘Ruby Roman’2022

    • Author(s)
      Mei Gao-Takai, Zen Lin, Yuta Sugiyama, Takane Katayama, Ai Shinmura, Hikaru Naito, Ayako Katayama-Ikegami
    • Journal Title

      The Horticulture Journal

      Volume: 91 Issue: 4 Pages: 476-488

    • DOI

      10.2503/hortj.UTD-371

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2023 Annual Research Report 2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Difference Between Coloration and Endogenous Abscisic Acid Accumulation Patterns in Two Red Grape Cultivars, ‘Aki Queen’ and ‘Ruby Roman’ (<i>Vitis labruscana</i> Bailey) Berries2022

    • Author(s)
      Katayama-Ikegami Ayako, Sugiyama Yuta, Katayama Takane, Sakamoto Akiko, Shimada Ryo, Miyazaki Chiho, Gao-Takai Mei
    • Journal Title

      The Horticulture Journal

      Volume: 91 Issue: 3 Pages: 312-321

    • DOI

      10.2503/hortj.UTD-342

    • NAID

      130008159377

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2022 Research-status Report 2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Suppression of red color development associated with anthocyanin accumulation in the epicarp of grape (Vitis labrusca x vinifera cv. Ruby Roman) berries caused by air temperature in daylight periods higher than 33 degrees C during maturation2021

    • Author(s)
      Kenichi Matsuda, Mei Gao-Takai, Ayaka Date, Takashi Suzuki
    • Journal Title

      SCIENTIA HORTICULTURAE

      Volume: 288 Pages: 110381-110381

    • DOI

      10.1016/j.scienta.2021.110381

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] Transcriptome analysis for ‘Ruby-Roman’ grapes grafted on diploid and tetraploid rootstocks2023

    • Author(s)
      Mei Gao-Takai
    • Organizer
      4th Asian Horticultural Congress
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ブドウ果実着色とABA蓄積パターンの遺伝性2021

    • Author(s)
      高居恵愛, 片山(池上)礼子, 片山(池上)礼子, 中嶋恭佑, 鈴木花野, 越野遼太郎
    • Organizer
      日本園芸学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 温度処理がブドウ‘ルビーロマン’と‘安芸クイーン’果粒の着色と果皮における植物ホルモンに及ぼす影響2021

    • Author(s)
      高居恵愛, 片山(池上)礼子, 松田賢一
    • Organizer
      日本園芸学会
    • Related Report
      2021 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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