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Analysis of neurophysiological mechanisms that regulate escape behavior in an environment-dependent manner

Research Project

Project/Area Number 21K06264
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44050:Animal physiological chemistry, physiology and behavioral biology-related
Research InstitutionKyoto University

Principal Investigator

Usui Tadao  京都大学, 生命科学研究科, 講師 (10324708)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords捕食回避行動 / 感覚ゲーティング / 疼痛緩和療法 / カルシウムイメージング / ショウジョウバエ / 神経ペプチド / 侵害逃避行動 / 環境依存性 / 寄生蜂 / 細胞内発火抑制 / カルシウム依存性カルシウム放出 / 複数感覚情報の統合 / 環境ストレス応答
Outline of Research at the Start

動物が天敵の襲撃を臨機応変に回避することは、個体の生存に直結する重要な本能行動である。申請者は、こうした逃避行動を調節する遺伝的・環境依存的な調節機構を明らかにするため、ショウジョウバエ野生型系統群を用いたゲノムワイド関連解析を実施し、これまでにGPIアンカー型タンパク質をコードする遺伝子 belly roll (bero) が逃避行動の迅速性を調節することを発見した。本研究では、bero 発現ニューロンによる環境依存的な行動調節機構と、Bero タンパク質の分子機能とを追究する。得られる知見は、生物の本能行動の環境調節機構に対する理解を深めることが期待される。

Outline of Final Research Achievements

We found that the Bero protein is essential for the formation of spontaneous and sustained neural activity (Ca2+ oscillations) of ABLK neurons, and that these Ca2+ oscillations antagonize the nociceptive response of ABLK neurons. This strongly suggested that sensory inputs other than nociceptive inputs were integrated with nociceptive inputs. In a paper published in the international journal eLife (Li et al., 2023), we found that neuropeptide receptors expressed in ABLK neurons functionally interact with Bero protein. We also found that the neuronal activity of ABLK neurons fluctuates in response to various environmental stresses, which is still being verified.

Academic Significance and Societal Importance of the Research Achievements

本研究課題で得られた成果は、動物の生存戦略の「かなめ」である捕食者からの回避行動について、これまで知られていなかった神経調節メカニズムを明らかにしたことである。従来は、捕食回避行動は定型的であり、調節される余地はあまりないと考えられてきた。しかし、本研究により、自然に存在する遺伝的な因子(bero遺伝子の多型)により捕食回避行動の迅速性が多様化していることが明らかになった。これに加えて、調節神経回路の動態が、さまざまな環境要因によっても変動することがわかってきた。回避行動は痛覚により起動されるため、本研究課題の成果は、ヒトの疼痛緩和療法を改善するための有用な情報を与えると期待できる。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (11 results)

All 2023 2022 2021 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (7 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Belly roll, a GPI-anchored Ly6 protein, regulates Drosophila melanogaster escape behaviors by modulating the excitability of nociceptive peptidergic interneurons2023

    • Author(s)
      Li Kai、Tsukasa Yuma、Kurio Misato、Maeta Kaho、Tsumadori Akimitsu、Baba Shumpei、Nishimura Risa、Murakami Akira、Onodera Koun、Morimoto Takako、Uemura Tadashi、Usui Tadao
    • Journal Title

      eLife

      Volume: 12 Pages: 1-31

    • DOI

      10.7554/elife.83856

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inter-organ Wingless/Ror/Akt signaling regulates nutrient-dependent hyperarborization of somatosensory neurons2023

    • Author(s)
      Kanaoka Yasutetsu、Onodera Koun、Watanabe Kaori、Hayashi Yusaku、Usui Tadao、Uemura Tadashi、Hattori Yukako
    • Journal Title

      eLife

      Volume: 12 Pages: 1-30

    • DOI

      10.7554/elife.79461

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] E-cadherin-dependent coordinated epithelial rotation on a two-dimensional discoidal pattern2022

    • Author(s)
      Shuangyu Luo, Kanji Furuya, Kimiya Matsuda, Yuma Tsukasa, Tadao Usui, Tadashi Uemura
    • Journal Title

      Genes to Cells

      Volume: 28 Issue: 3 Pages: 1-13

    • DOI

      10.1111/gtc.13001

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Hemolymph osmolality gates nociceptive transmission via atypical GPCR signaling2023

    • Author(s)
      Yuma Tsukasa, Misato Kurio, Kaho Maeta, Kai Li, Tadashi Uemura and Tadao Usui
    • Organizer
      Asia Pacific Drosophila Neurobiology Conference 3
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fluid osmolality gates nociceptive escape behaviors in Drosophila larvae2023

    • Author(s)
      Yuma Tsukasa, Misato Kurio, Kaho Maeta, Kai Li, Tadashi Uemura and Tadao Usui
    • Organizer
      The 46th Annual Meeting of the Molecular Biology Society of Japan
    • Related Report
      2023 Annual Research Report
  • [Presentation] Hemolymph osmolality gates nociceptive transmission via atypical GPCR signaling2023

    • Author(s)
      Yuma Tsukasa, Misato Kurio, Kaho Maeta, Kai Li, Tadashi Uemura and Tadao Usui
    • Organizer
      The 20th Biostudies Student Symposium
    • Related Report
      2023 Annual Research Report
  • [Presentation] The novel Ly6/α-Neurotoxin family protein, Belly roll, regulates the nociceptive transmission in response to the fluid osmolality2022

    • Author(s)
      Yuma Tsukasa, Kai Li, Misato Kurio, Kaho Maeta, Akimitsu Tsumadori, Shumpei Baba, Risa Nishimura, Koun Onodera, Takako Morimoto, Tadashi Uemura, Tadao Usui
    • Organizer
      第45回日本神経科学大会(NEURO2022)
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] The internal state-dependent modulation of the nociceptive transmission2022

    • Author(s)
      Yuma Tsukasa, Kai Li, Misato Kurio, Kaho Maeta, Akimitsu Tsumadori, Shumpei Baba, Risa Nishimura, Koun Onodera, Takako Morimoto, Tadashi Uemura, Tadao Usui
    • Organizer
      第15回日本ショウジョウバエ研究集会
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Elucidation of the internal state-dependent modulation of the nociceptive escape behavior2021

    • Author(s)
      Tadao Usui
    • Organizer
      Neurogenetics of the Drosophila larva
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Elucidation of the internal state-dependent modulation of the nociceptive escape behavior2021

    • Author(s)
      Tadao Usui
    • Organizer
      International Symposium on Development and Plasticity of Neural System
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Remarks] 逃避行動が遺伝要因や環境要因により調節されるメカニズムの理解

    • URL

      https://www.cellpattern.lif.kyoto-u.ac.jp/research/research-usui/

    • Related Report
      2022 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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