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Function of exosomes in breast cancer: The novel mechanism of exosome secretion by trace metals

Research Project

Project/Area Number 21K15397
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 49020:Human pathology-related
Research InstitutionTohoku University

Principal Investigator

Iwabuchi Erina  東北大学, 医学系研究科, 助教 (70837278)

Project Period (FY) 2021-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords乳癌 / 亜鉛トランスポーター / タンパク質間相互作用
Outline of Research at the Start

エクソソームは癌細胞や間質の正常細胞から分泌され,癌の進展に関わることが報告されており,個別化医療実施のための解析対象として注目されている.本研究では,申請者がこれまで進めてきたエクソソーム分泌に関わるタンパク質間相互作用 (PPI: protein-protein interaction) について,制御機構に関わる因子として微量金属元素に着目した.さらに,分泌されたエクソソームが乳癌細胞に与える影響を検討し,PPIと微量金属元素との関係およびエクソソームの機能を明らかにする.

Outline of Final Research Achievements

In this study, immunohistochemical analysis in breast cancer cases revealed a significant inverse association between the expression of zinc transporters and some clinicopathological factors that are indicators of breast cancer malignancy. Furthermore, in vitro studies suggested that zinc transporters are involved in the inhibition of cell migration via the signaling pathway of growth factors. Our results indicated that the zinc transporter could suppress the progression of breast cancer. In addition, ZnCl2 treatment of breast cancer cells increased the expression of protein-protein interaction-related factors involved in exosome secretion, indicating the possibility that zinc is involved in exosome secretion.

Academic Significance and Societal Importance of the Research Achievements

本研究では細胞内小器官内外の輸送に関与する亜鉛トランスポーターが乳癌細胞の悪性化を抑制することが示唆され,亜鉛トランスポーターの乳癌に対する新しい知見を得た.また,その抑制機序として成長因子のシグナル伝達経路を介して機能していることも示した.本研究により乳癌の新しい進展経路を明らかにし,今後の乳癌の診断や治療の発展に寄与すると考えられる.

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (8 results)

All 2022 2021

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] Visualization of the protein-protein interactions of hormone receptors in hormone-dependent cancer research2022

    • Author(s)
      Iwabuchi Erina, Miki Yasuhiro, Suzuki Takashi, Sasano Hironobu
    • Journal Title

      Endocrine Oncology

      Volume: 2 Issue: 1 Pages: R132-R142

    • DOI

      10.1530/eo-22-0059

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Visualization of Protein-Protein Interactions in Breast Cancer: Deployment Study in Pathological Examination2021

    • Author(s)
      Iwabuchi Erina、Miki Yasuhiro、Sasano Hironobu
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 54 Issue: 6 Pages: 177-183

    • DOI

      10.1267/ahc.21-00084

    • NAID

      130008132650

    • ISSN
      0044-5991, 1347-5800
    • Year and Date
      2021-12-24
    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] Zinc transporter ZnT5 Involvement in Epithelial Mesenchymal Transition in Breast Cancer2022

    • Author(s)
      Erina Iwabuchi, Yasuhiro Miki, Junyao Xu, Takanori Ishida, Hironobu Sasano
    • Organizer
      ENDO2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Detection of somatostatin receptors using ligand derivative staining in breast cancer2022

    • Author(s)
      Erina Iwabuchi, Kento Iida, Yasuhiro Miki, Koki Hasegawa, Takumi Fukasawa, Takanori Ishida, Hironobu Sasano, Takashi Suzuki
    • Organizer
      第81回 日本癌学会学術総会
    • Related Report
      2022 Annual Research Report
  • [Presentation] The exosome marker CD63 and Caveolin-1 in breast cancer patients2021

    • Author(s)
      Erina Iwabuchi, Yasuhiro Miki, Ayako Kanai, Takanori Ishida, Hironobu Sasano
    • Organizer
      American Association for Cancer Research Annual Meeting 2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] タンパク質間相互作用可視化技術の乳癌病理検査への展開2021

    • Author(s)
      岩渕英里奈
    • Organizer
      第62回日本組織細胞化学総会・学術集会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Association between Caveolin-1 and exosomes in breast cancer2021

    • Author(s)
      Erina Iwabuchi, Yasuhiro Miki, Ayako Kanai, Takanori Ishida, Hironobu Sasano
    • Organizer
      第80回 日本癌学会学術総会
    • Related Report
      2021 Research-status Report
  • [Presentation] タンパク質間相互作用可視化技術 ~新しい乳癌病理検査への展望~2021

    • Author(s)
      岩渕英里奈
    • Organizer
      仙台内分泌フォーラム2022
    • Related Report
      2021 Research-status Report
    • Invited

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Published: 2021-04-28   Modified: 2025-03-27  

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