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Evolutional strategy for the establishment of angiosperm-type chloroplast NDH

Research Project

Project/Area Number 22247005
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKyoto University

Principal Investigator

SHIKANAI TOSHIHARU  京都大学, 理学(系)研究科(研究院), 教授 (70273852)

Co-Investigator(Renkei-kenkyūsha) TSUGEKI Ryuuji  京都大学, 大学院理学研究科, 助教 (50303805)
NISHIMURA Yoshiki  京都大学, 大学院理学研究科, 助教 (70444099)
Project Period (FY) 2010-04-01 – 2013-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥43,940,000 (Direct Cost: ¥33,800,000、Indirect Cost: ¥10,140,000)
Fiscal Year 2012: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2011: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2010: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Keywords葉緑体 / NDH / サイクリック電子伝達 / 光合成 / アセンブリ / 進化 / シロイヌナズナ / ゼニゴケ / サイクイック電子伝達 / レドックス調節 / 電子伝達 / 分子シャペロン / アッセンブリー / コケ植物
Research Abstract

We discovered that a novel protein, NdhS is necessary for the high-affinity binding of chloroplast NDH with ferredoxin. Chloroplast NDH is not NAD(P)H dehydrogenase but antimycin A-resistant ferredoxin-dependent plastoquinone reductase (FQR). We also clarified the assembly process of subcomplex A of chloroplast NDH in Arabidopsis with the discovery of several assembly factors specifically required for this process. We further discovered that in Marchantia chloroplast NDH does not form the supercomplex with photosystem I and is involved in the redox homeostasis in chloroplasts at low light intensity.

Report

(4 results)
  • 2013 Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (41 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (21 results) (of which Peer Reviewed: 16 results,  Open Access: 1 results) Presentation (19 results) (of which Invited: 2 results) Book (1 results)

  • [Journal Article] Central role of cyclic electron transport around photosystem I in the regulation of photosynthesis2014

    • Author(s)
      Shikanai T
    • Journal Title

      Curr. Opin. Biotech.

      Volume: 26 Pages: 25-30

    • DOI

      10.1016/j.copbio.2013.08.012

    • Related Report
      2013 Final Research Report
  • [Journal Article] In planta mutagenesis of Src homology 3 domain-like fold of NdhS, a ferredoxin-binding subunit of the chloroplast NADH dehydrogenase-like complex in Arabidopsis. A conserved Arg-193 plays a critical role in ferredoxin binding2013

    • Author(s)
      Yamamoto H. and Shikanai T
    • Journal Title

      J. Biol. Chem.

      Volume: 288 Issue: 51 Pages: 36328-36337

    • DOI

      10.1074/jbc.m113.511584

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antimycin A-like molecules inhibit cyclic electron transport around photosystem I in ruptured chloroplasts2013

    • Author(s)
      Taira Y., Okegawa Y., Sugimoto K., Abe M., Miyoshi H. and Shikanai T
    • Journal Title

      FEBS Open Bio.

      Volume: 3 Issue: 1 Pages: 406-410

    • DOI

      10.1016/j.fob.2013.09.007

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A single amino acid alteration in PGR5 confers resistance to antimycin A in cyclic electron transport around PSI2013

    • Author(s)
      Sugimoto K., Okegawa Y., Tohri A., Long T.A., Sarah F.S., Hisabori T. and Shikanai T
    • Journal Title

      Plant Cell Physiol.

      Volume: 54 Issue: 9 Pages: 1525-1534

    • DOI

      10.1093/pcp/pct098

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutagenesis of Individual Pentatricopeptide Repeat Motifs Affects RNA Binding Activity and Reveals Functional Partitioning of Arabidopsis PROTONGRADIENT REGULATION32013

    • Author(s)
      Fujii S, Sato N, Shikanai T
    • Journal Title

      Plant Cell

      Volume: 25 Issue: 8 Pages: 1-11

    • DOI

      10.1105/tpc.113.112193

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PGR 5-dependent cyclic electron transport around PSI contributes to the redox homeostasis in chloroplasts rather than CO_2 fixation and biomass production in rice2012

    • Author(s)
      Nishikawa Y, Yamamoto H, Okegawa Y, Wada S, Sato N, Taira Y, Sugimoto K, Makino A and Shikanai T
    • Journal Title

      Plant Cell Physiol

      Volume: 53 Issue: 12 Pages: 2117-2126

    • DOI

      10.1093/pcp/pcs153

    • NAID

      10031145641

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Composition and physiological function of the chloroplast NADH dehydrogenase-like complex in Marchantia polymorpha2012

    • Author(s)
      Ueda M., Kuniyoshi T., Yamamoto Y., Sugimoto K., Ishizaki K., Kohchi T., Nishimura Y. and Shikanai T
    • Journal Title

      Plant J.

      Volume: 72 Issue: 4 Pages: 683-693

    • DOI

      10.1111/j.1365-313x.2012.05115.x

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multistep assembly of chloroplast NADH dehydrogenase-like subcomplex A requires several nucleus-encoded proteins including CRR41 and CRR42, in Arabidopsis2012

    • Author(s)
      Peng L, Fukao Y, Fujiwara M, Shikanai T
    • Journal Title

      Plant Cell

      Volume: 24 Issue: 1 Pages: 202-214

    • DOI

      10.1105/tpc.111.090597

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure of the chloroplast NADH dehydrogenase-like complex : nomenclature for nuclear-encoded subunits2011

    • Author(s)
      Ifuku K, Endo T, Shikanai T, Aro E-M
    • Journal Title

      Plant Cell Physiol

      Volume: 52 Issue: 9 Pages: 1560-1568

    • DOI

      10.1093/pcp/pcr098

    • NAID

      10029714253

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An Src homology 3 domain-like fold protein forms a ferredoxin-binding site for the chloroplast NADH dehydrogenase-like complex in Arabidopsis2011

    • Author(s)
      Yamamoto H., Peng L., Fukao Y. and Shikanai T
    • Journal Title

      Plant Cell

      Volume: 23 Issue: 4 Pages: 1480-1493

    • DOI

      10.1105/tpc.110.080291

    • Related Report
      2013 Final Research Report
  • [Journal Article] A chaperonin subunit with unique structures is essential for folding of a specific substrate2011

    • Author(s)
      Peng L., Fukao Y., Myouga F., Motohashi R., Shinozaki K. and Shikanai T
    • Journal Title

      PLoS Biol.

      Volume: 9 Issue: 4 Pages: e1001040-e1001040

    • DOI

      10.1371/journal.pbio.1001040

    • Related Report
      2013 Final Research Report
  • [Journal Article] Supercomplex formation with photosystem I is required for the stabilization of the chloroplast NADH dehydrogenase-like complex in Arabidopsis2011

    • Author(s)
      Peng L. and Shikanai
    • Journal Title

      Plant Physiol.

      Volume: 155 Issue: 4 Pages: 1629-1639

    • DOI

      10.1104/pp.110.171264

    • Related Report
      2013 Final Research Report
  • [Journal Article] Structure and biogenesis of the chloroplast NAD(P)H dehydrogenase complex2011

    • Author(s)
      Peng L., Yamamoto H. and Shikanai T
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1807 Issue: 8 Pages: 945-953

    • DOI

      10.1016/j.bbabio.2010.10.015

    • Related Report
      2013 Final Research Report
  • [Journal Article] PROTON GRADIENT REGULATION 3 recognizes multiple targets with limited similarity and mediates translation and RNA stabilization in plastids2011

    • Author(s)
      Cai W, Okuda K, Peng L, Shikanai T
    • Journal Title

      Plant J

      Volume: 67 Issue: 2 Pages: 318-327

    • DOI

      10.1111/j.1365-313x.2011.04593.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Supercomplex formation with photosystem I is required for the stabilization of the chloroplast NADH dehydrogenase-like complex in Arabidopsis.2011

    • Author(s)
      Peng L, Shikanai T
    • Journal Title

      Plant Physiology

      Volume: 155 Pages: 1629-1639

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A chaperonin subunit with unique structures is essential for folding of a specificsubstrate.2011

    • Author(s)
      Peng L, Fukao Y, Myouga F, Motohashi R, Shinozaki K, Shikanai T
    • Journal Title

      PLoS Biology

      Volume: 9

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Src homology 3 domain-like fold protein forms a ferredoxin-binding site for the chloroplast NADH dehydrogenase-like complex in Arabidopsis.2011

    • Author(s)
      Yamamota H, Peng L, Fukao Y, Shikanai T.
    • Journal Title

      The Plant Cell

      Volume: 22(印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure and biogenesis of the chloroplast NAD(P)H dehydrogenase complex2011

    • Author(s)
      Peng L, Yamamoto H, Shikanai T
    • Journal Title

      Biochimica Biophysica Acta

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chloroplast stromal proteins, CRR6 and CRR7, are required for assembly of the NAD(P)H dehydrogenase subcomplex A in Arabidopsis.2010

    • Author(s)
      Peng L, Cai W, Shikanai T
    • Journal Title

      The Plant Journal

      Volume: 63 Pages: 203-211

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Physiological links among alternative electron transport pathways reducing and oxidizing plastoquinone in Arabidopsis.2010

    • Author(s)
      Okegawa Y, Kobayashi Y, Shikanai T
    • Journal Title

      The Plant Journal

      Volume: 63 Pages: 458-468

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] De novo biosynthesis of fatty acids plays critical roles in the response of the photosynthetic machinery to low temperature in Arabidopsis.2010

    • Author(s)
      Takami T, Sibata M, Kobayashi Y, Shikanai T
    • Journal Title

      Plant Cell Physiology

      Volume: 51 Pages: 1265-1275

    • NAID

      10027351358

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Machinery and function of PSI cyclic electron transport2014

    • Author(s)
      Shikanai T
    • Organizer
      2nd Kyoto-Bristol Symposium
    • Place of Presentation
      Kyoto
    • Year and Date
      2014-01-10
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of photosynthetic electron transport2013

    • Author(s)
      Shikanai T
    • Organizer
      NC-CARP•CREST/さきがけ国際シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2013-11-08
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of photosynthesis by PSI cyclic electron transport2013

    • Author(s)
      Shikanai T
    • Organizer
      PhD Summer School : New Frontiers in Photosynthesis
    • Place of Presentation
      San Michele all'Adige, Italy
    • Year and Date
      2013-07-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of photosynthesis by PSI cyclic electron transport2013

    • Author(s)
      Shikanai T
    • Organizer
      24th International Conference on Arabidopsis Research
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2013-06-25
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
  • [Presentation] Regulation of photosynthesis by PSI cyclic electron transport2013

    • Author(s)
      Shikanai T
    • Organizer
      3^<rd> International Symposium on Chloroplast Genomics and Genetic Engineering
    • Place of Presentation
      New Brunswick, USA
    • Year and Date
      2013-05-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Cyclic electron flow : new structural components and regulation2012

    • Author(s)
      Shikanai T
    • Organizer
      IPMB 2012
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2012-10-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Assembly of the chloroplast NDH complex2012

    • Author(s)
      Shikanai T
    • Organizer
      The 11th Nordic Photosynthesis Congress
    • Place of Presentation
      Naantali, Finland
    • Year and Date
      2012-09-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Chloroplast NADH dehydrogenase- like complex : function and assembly2012

    • Author(s)
      Shikanai T
    • Organizer
      Gordon Research Conference, Mitochondria & Chloroplasts
    • Place of Presentation
      Smithfield, USA
    • Year and Date
      2012-08-01
    • Related Report
      2013 Final Research Report
  • [Presentation] Structure and assembly of the chloroplast NDH complex2012

    • Author(s)
      Shikanai T
    • Organizer
      NU Plant Science Symposium 2012
    • Place of Presentation
      Jinju, Korea
    • Year and Date
      2012-05-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of photosynthetic electron transport : Structure and assembly of thechloroplast NDH complex2011

    • Author(s)
      Shikanai T, Peng L, Yamamoto H
    • Organizer
      Japanese-Finish seminar 2011
    • Place of Presentation
      岡山ロイヤルホテル(岡山市)
    • Year and Date
      2011-03-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Machinery and assembly of the chloroplast NDH complex.2011

    • Author(s)
      Shikanai T, Peng L, Yamamoto H
    • Organizer
      2011 Switzerland-Japan Workshop
    • Place of Presentation
      Ollon, Swiss
    • Year and Date
      2011-01-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Machinery and assembly of PSI cyclic electron transport2011

    • Author(s)
      Shikanai T
    • Organizer
      Gordon Research Conference, Photosynthesis
    • Place of Presentation
      Davidson, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Machinery and assembly of the chloroplast NDH complex2011

    • Author(s)
      Shikanai T
    • Organizer
      witzerland-Japan Workshop
    • Place of Presentation
      Ollon, Swiss
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of photosynthetic electron transport by PSI cyclic electron transport2011

    • Author(s)
      Shikanai T
    • Organizer
      The 15^<th> International Congress of Photosynthesis
    • Place of Presentation
      Beijing, China
    • Related Report
      2013 Final Research Report
  • [Presentation] Machinery and assembly of PSI cyclic electron transport2011

    • Author(s)
      Shikanai T
    • Organizer
      Gordon Reserch Conference Photosynthesis
    • Place of Presentation
      Davidson, NC, USA(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Regulation of photosynthetic electron transport by PSI cyclic electron transport.2010

    • Author(s)
      Shikanai T
    • Organizer
      The 15^<th> International Congress of Photosynthesis
    • Place of Presentation
      北京(中国)
    • Year and Date
      2010-08-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Structure and function of machinery of photosystem I cyclic electron transport2010

    • Author(s)
      Shikanai T
    • Organizer
      APRU Research Symposium
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of photosynthesis by PSI cyclic electron transport

    • Author(s)
      Shikanai T
    • Organizer
      3rd International Symposium on Chloroplast Genomics and Genetic Engineering
    • Place of Presentation
      New Brunswick, USA
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Regulation of photosynthesis by PSI cyclic electron transport

    • Author(s)
      Shikanai T
    • Organizer
      PhD Summer School: New Frontiers in Photosynthesis
    • Place of Presentation
      San Michele all’Adige, Italy
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] Regulation of photosynthetic electron transport. In The Chloroplast : Basics and Application2010

    • Author(s)
      Shikanai T
    • Publisher
      Springer
    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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