Sleep and arousal regulation in Drosophila melanogaster

• Project/Area Number: 22300132
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fusional basic brain science
• Research Institution: Kumamoto University
• Principal Investigator: KUME Kazuhiko 熊本大学, 発生医学研究所, 准教授 (30251218)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2010: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 睡眠 / 覚醒 / 概日周期 / ショウジョウバエ / ドーパミン / 時系列解析 / モザイク解析 / 代謝 / 加齢 / 寿命 / 温度嗜好性 / 記憶 / 麻酔 / 摂食 / ドパミン

Research Abstract

Using Drosophila melanogaster as a model animal for sleep research,we identified calcineurin and others as novel sleep related genes and showed therelationship between sleep and memory retention. By the analysis of dopamine functions,which is an arousal regulator, we showed its role in rest length regulation, metabolismand temperature preference. We also identified arousal regulating dopamine circuit infly brain at the single cell level.

Research Products

• [Journal Article] Identification of a dopamine pathwaythat regulates sleep and arousal inDrosophila (2012)
  • Author(s): T. Ueno, J. Tomita, H. Tanimoto, K.Endo, K. Ito, S. Kume, K. Kume
  • Journal Title: Nat Neurosci Volume: 15 Issue: 11 Pages: 1516-1523
  • DOI: 10.1038/nn.3238
  • Peer Reviewed
• [Journal Article] Pan-neuronal knockdown of the c-JunN-terminal Kinase (JNK) results in areduction in sleep and longevity inDrosophila (2012)
  • Author(s): K. Takahama, J. Tomita, T. Ueno, M.Yamazaki, S. Kume, K. Kume
  • Journal Title: Biochem Biophys Res Commun Volume: 417 Issue: 2 Pages: 807-11
  • DOI: 10.1016/j.bbrc.2011.12.040
  • Peer Reviewed
• [Journal Article] High calorie diet augmentsage-associated sleep impairment inDrosophila (2012)
  • Author(s): M. Yamazaki, J. Tomita, K. Takahama,T. Ueno, M. Mitsuyoshi, E. Sakamoto, S.Kume, K. Kume
  • Journal Title: Biochem Biophys Res Commun Volume: 417 Issue: 2 Pages: 812-6
  • DOI: 10.1016/j.bbrc.2011.12.041
  • Peer Reviewed
• [Journal Article] Dopamine modulates the rest period length without perturbation of itspower law distribution in Drosophila melanogaster (2012)
  • Author(s): T. Ueno, N. Masuda, S. Kume, K. Kume
  • Journal Title: PLoS ONE Volume: 7 Issue: 2 Pages: e32007-e32007
  • DOI: 10.1371/journal.pone.0032007
  • Peer Reviewed
• [Journal Article] Dopamine modulates metabolic rate andtemperature sensitivity in Drosophila melanogaster (2012)
  • Author(s): T. Ueno, J. Tomita, S. Kume, K. Kume
  • Journal Title: PLoS One Volume: 7 Issue: 2 Pages: e31513-e31513
  • DOI: 10.1371/journal.pone.0031513
  • Peer Reviewed
• [Journal Article] Isolation of the bioactive peptides CCHamide-1 and CCHamide-2 from Drosophila and their putative role in appetite regulation as ligands for G protein-coupled receptors (2012)
  • Author(s): Ida T, Takahashi T, Tominaga H, Sato T, Sano H, Kume K, Ozaki M, Hiraguchi T, Shiotani H, Terajima S, Nakamura Y, Mori K, Yoshida M, Kato J, Murakami N, Miyazato M, Kangawa K, Kojima M
  • Journal Title: Front Endocrinol (Lausanne) Volume: 3 Pages: 177-177
  • DOI: 10.3389/fendo.2012.00177
  • Peer Reviewed
• [Journal Article] Monozygotic twins concordant for Kleine-Levin syndrome. (2012)
  • Author(s): Ueno, T.
  • Journal Title: BMC Neurol Volume: 12 Issue: 1 Pages: 31-31
  • DOI: 10.1186/1471-2377-12-31
  • Peer Reviewed
• [Journal Article] Pan-Neuronal Knockdown of CalcineurinReduces Sleep in the Fruit Fly,Drosophila melanogaster (2011)
  • Author(s): J. Tomita, M. Mitsuyoshi, T. Ueno, Y.Aso, H. Tanimoto, Y. Nakai, T. Aigaki, S.Kume, K. Kume
  • Journal Title: J Neurosci Volume: 31 Issue: 37 Pages: 13137-46
  • DOI: 10.1523/jneurosci.5860-10.2011
  • Peer Reviewed
• [Journal Article] Calcineurin and Its Regulator Sra/DSCR1Are Essential for Sleep in Drosophila (2011)
  • Author(s): Y. Nakai, J. Horiuchi, M. Tsuda, S.Takeo, S. Akahori, T. Matsuo, K. Kume, T.Aigaki
  • Journal Title: J Neurosci Volume: 31 Issue: 36 Pages: 12759-66
  • DOI: 10.1523/jneurosci.1337-11.2011
  • Peer Reviewed
• [Journal Article] Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptide Y-like receptor in Drosophila (2011)
  • Author(s): Ida T, Takahashi T, Tominaga H, Sato T, Kume K, Ozak Mi, Hiraguchi T, Maeda T, Shiotani H, Terajima S, Sano H, Mori K, Yoshida M, Miyazato M, Kato J, Murakami N, Kangawa K, Kojima M
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Issue: 4 Pages: 872-877
  • DOI: 10.1016/j.bbrc.2011.06.081
  • Peer Reviewed
• [Journal Article] Identification of the endogenous cysteine-rich peptide trissin, ligand for an orphan G-protein-coupled receptor in Drosophila (2011)
  • Author(s): Ida T, Takahashi T, Tominaga H, Sato T, Kume K, Yoshizawa-Kumagae K, Nishio H, Kato J, Murakami N, Miyazato M, Kangawa K, Kojima M
  • Journal Title: Biochem Biophys Res Commun Volume: 414 Issue: 1 Pages: 44-48
  • DOI: 10.1016/j.bbrc.2011.09.018
  • Peer Reviewed
• [Journal Article] Behavioral consequences of dopaminedeficiency in the Drosophila centralnervous system (2011)
  • Author(s): T. Riemensperger, G. Isabel, H.Coulom, K. Neuser, L. Seugnet, K. Kume,et al
  • Journal Title: Proc Natl Acad Sci U S A Volume: 108 Issue: 2 Pages: 834-9
  • DOI: 10.1073/pnas.1010930108
  • Peer Reviewed
• [Journal Article] Behavioral consequences of dopamine deficiency in the Drosophila central nervous system. (2011)
  • Author(s): Riemensperger T., et al.
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. Volume: 108 Pages: 834-839
  • Peer Reviewed
• [Presentation] Dopaminergic regulation ofarousal in Drosophila (2012)
  • Author(s): K. Kume
  • Organizer: バイオジェニックアミン国際シンポジウム
  • Place of Presentation: ベルリン自由大学、ドイツ、Berlin
• [Book] 時間生物学 (2012)
  • Author(s): 海老原史樹文・吉村崇編
  • Publisher: 化学同人
• [Remarks] プレス発表熊本大学発生医学研究所ホームページにて
  • URL: http://www.imeg.kumamoto-u.ac.jp/topics/ueno2012.html
• [Remarks] 上野太郎研究員・粂和彦准教授ら（多能性幹細胞分野）の研究成果が新聞発表されました
  • URL: http://www.imeg.kumamoto-u.ac.jp/topics/ueno2012.html
• [Remarks]
  • URL: http://www.imeg.kumamoto-u.ac.jp/divisions/stem_cell_biology/
• [Patent(Industrial Property Rights)] ショウジョウバエ新規生理活性ペプチドの発見とその作用 (2010)
  • Inventor(s): 井田隆徳,児島将康,粂 和彦
  • Industrial Property Rights Holder: 井田隆徳,児島将康,粂 和彦、学校法人久留米大学、国立大学法人熊本大学
  • Industrial Property Number: 2010-045816
  • Filing Date: 2010-03-02