Cryo-bioimaging by using coherent X-ray diffraction
Project/Area Number |
22310075
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Nanomaterials/Nanobioscience
|
Research Institution | Hokkaido University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
MAESHIMA Kazuhiro 国立遺伝学研究所, 構造遺伝学研究センター, 教授 (00392118)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
Keywords | コヒーレントX線 / 染色体 / X線回折顕微法 / ナノ構造解析 / 位相コントラストイメージング / オルガネラ / 高次構造 / 位相回復 / コヒーレントX線 / X線回折顕微法 |
Research Abstract |
We revised the established theory of the structure of human chromosomes by X-ray scattering experiments conducted using SPring-8. Experimental data indicate irregularly folded nucleosome fibers without a 30-nm chromatin structure or regularly folding of chromatin fibers. We also proposed a new coherent imaging method using a confined illumination, which can be applicable also to frozen-hydrated biological samples with extended ice layer, to overcome the limitation of conventional data analysis algorithm in X-ray diffraction microscopy.
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Report
(4 results)
Research Products
(106 results)