Analysis of RACK function in specific nascent peptide-dependent translation arrest

• Project/Area Number: 22370062
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Tohoku University
• Principal Investigator: INADA Toshifumi 東北大学, 大学院・薬学研究科, 教授 (40242812)
• Co-Investigator(Renkei-kenkyūsha): HOSHINO Shin-ichi 名古屋市立大学, 大学院・薬学研究科, 教授 (40219168)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2010: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: mRNA 品質管理 / 翻訳アレスト / 新生ポリペプチド鎖 / mRNA 分子内切断 / ユ ビキチン化 / プロテアソーム / リボソーム / リボソーム解離因子 / mRNA品質管理 / 異常RNA分解 / 異常タンパク質分解 / ユビキチン化 / RNA / 翻訳 / 品質管理 / タンパク質分解

Research Abstract

The receptor for activated C kinase (RACK1) was identified as a factor required for nascent peptide-dependent translation arrest, and the binding of RACK1 to 40S subunit is crucial for translation repression by poly-basic amino acid sequence. Two E3 ubiquitin ligases, Ltn1 and Not4, are involved in proteasomal protein degradation coupled to translation arrest, and translation arrest by RACK1 is required for Ltn1-depepdnet protein degradation.

Research Products

• [Journal Article] A ribosome-bound quality control complex triggers nascent peptides and signals translation stress (2012)
  • Author(s): Brandman, O., Ornstein, JS., Wong, D., Larson, A., Williams, C. C, Li, GW., Zhou, S., King, D., Shen, P S, Weibezahn, J., Dunn, J.q Rouskin, S., Inada, T., Frost, A., Weissman, JS
  • Journal Title: Cell Volume: 151 Issue: 5 Pages: 1042-1054
  • DOI: 10.1016/j.cell.2012.10.044
  • Peer Reviewed
• [Journal Article] Dom34:Hbs1 Plays a General Role in Quality-Control Systems by Dissociation of a Stalled Ribosome at the 3' End of Aberrant mRNA (2012)
  • Author(s): Tatsuhisa Tsuboi, et al
  • Journal Title: Molecular Cell Volume: 46 Issue: 4 Pages: 1-12
  • DOI: 10.1016/j.molcel.2012.03.013
  • Peer Reviewed
• [Journal Article] Roles of dom34 : hbs1 in nonstop protein clearance from translocators for normal organelle protein influx. (2012)
  • Author(s): Toshiaki Izawa, Tatsuhisa Tsuboi, Kazushige Kuroha, et al.
  • Journal Title: Cell Reports Volume: 2 Issue: 3 Pages: 447-453
  • DOI: 10.1016/j.celrep.2012.08.010
  • Peer Reviewed
• [Journal Article] RACK1 stimulates nascent polypeptide-dependent translation arrest (2010)
  • Author(s): Kuroha, K., Akamatsu, M., Dimitrova, L, lto, T.,Kato, Y Shirahige, K. and lnada. T
  • Journal Title: EMBO. Rep Volume: 11 Issue: 12 Pages: 956-961
  • DOI: 10.1038/embor.2010.169
  • Peer Reviewed
• [Journal Article] Dual functions of yeast tRNA ligase in the unfolded protein response: unconventional cytoplasmic splicing of HAC1 pre-mRNA is not sufficient to release translational attenuation (2010)
  • Author(s): Mori T, Ogasawara C, lnada, T, Englert M, Beier H, Takezawa M, Endo T, Yoshihisa T
  • Journal Title: Mol. Biol. Cell Volume: 21 Issue: 21 Pages: 3722-3734
  • DOI: 10.1091/mbc.e10-08-0693
  • Peer Reviewed
• [Journal Article] Stmctural Basis for mRNA Surveillance by Archaeal Pelota and GTP-bound EF1α Complex (2010)
  • Author(s): Kobayashi, K. Kikuno,1. Kuroha, K. Saito, K. Ito, K. Ishitani, R. Inada. T. and Nureki, O
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 107 Issue: 41 Pages: 17575-17579
  • DOI: 10.1073/pnas.1009598107
  • Peer Reviewed
• [Journal Article] RACK1 stimulates nascent polypeptide-dependent translation arrest (2010)
  • Author(s): Kuroha, K., Akamatsu, M., Dimitrova, L., Ito, T., Kato, Y.Shirahige, K., Inada, T.
  • Journal Title: EMBO Reports Volume: 11 Pages: 956-961
  • Peer Reviewed
• [Journal Article] Structural Basis for mRNA Surveillance by Archaeal Pelota and GTP-bound EF1α Complex (2010)
  • Author(s): Kobayashi, K., Kikuno, I., Kuroha, K., Saito, K., Ito, K., Ishitani, R., Inada, T., Nureki, O.
  • Journal Title: Proc.Natl.Acad.Sci.USA Volume: 107 Pages: 17575-17579
  • Peer Reviewed
• [Journal Article] Tethering of poly(A) binding protein interferes with non-translated mRN A decay from 5' end in yeast (2010)
  • Author(s): Tsuboi, T., Inada, T
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 33589-33601
  • Peer Reviewed
• [Journal Article] Dual functions of yeast tRNA ligase in the unfolded protein response : unconventional cytoplasmic splicing of HAC1 pre-mRNA is not sufficient to release translational attenuation (2010)
  • Author(s): Mori, T., Ogasawara, C., Inada, T., Englert, M., Beier, H., Takezawa, M., Endo, T., Yoshihisa, T.
  • Journal Title: Mol.Biol.Cell Volume: 21 Pages: 3722-3734
  • Peer Reviewed
• [Presentation] 終止コドン非依存サブユニット解離による異常mRNA分解促進機構 (2012)
  • Author(s): 坪井達久、池内健、黒羽一誠、鹿島勲、工藤和兵、牧野志保、井上絵里、稲田利文
  • Organizer: Ribosome meeting
  • Place of Presentation: 広島、日本
  • Year and Date: 2012-03-16
• [Presentation] Novel roles of Dom34:Hbs1 and RACK1 in mRNA quality control systems (2012)
  • Author(s): 稲田利文
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Protein quality control systems coupled with No-Go and Non-Stop mRNA surveillance in yeast (2012)
  • Author(s): Inada, T
  • Organizer: EMBO meeting on 'Quality control'
  • Place of Presentation: EMBL, Heidelberg, Germany
• [Presentation] Dom34:Hbs1 Plays a General Role in Quality Control Systems by Dissociation of a Stalled Ribosome at the 3' End of Aberrant mRNA (2012)
  • Author(s): Inada, T
  • Organizer: Protein synthesis and translational control
  • Place of Presentation: Cold Spring Harbor, NewYork, USA
• [Presentation] Quality control of mRNA and protein in eukaryotes (2012)
  • Author(s): Inada, T
  • Organizer: The 22nd CDB meeting on "RNA Sciences in Cell and Developmental Biology II"
  • Place of Presentation: Kobe, Japan
• [Presentation] Stalled ribosomes induce mRNA and protein quality control systems (2012)
  • Author(s): Inada, T
  • Organizer: Jacques Monod conferences 25th anniversary
  • Place of Presentation: Roscoff, France
• [Presentation] Dom34:Hbs1 Plays a General Role in Quality Control Systems by Dissociation of a Stalled Ribosome at the 3’ End of Aberrant mRNA (2012)
  • Author(s): Toshifumi Inada
  • Organizer: Protein synthesis and translational control
  • Place of Presentation: CSH, NY, USA
• [Presentation] Protein quality control systems coupled with No-Go and Non-Stop mRNA surveillance in yeast (2012)
  • Author(s): Toshifumi Inada
  • Organizer: Quality control
  • Place of Presentation: Heidelberg, Germany
• [Presentation] Stalled ribosomes induce the degradation of aberrant mRNA and protein (2012)
  • Author(s): Toshifumi Inada
  • Organizer: FASB meeting mechanism of mRNA decay
  • Place of Presentation: Colorado, USA
• [Presentation] The mechanism of quality control coupled with translation arrest in yeast (2011)
  • Author(s): Toshifumi Inada
  • Organizer: ISNAC2011
  • Place of Presentation: 札幌、日本(招待講演)
  • Year and Date: 2011-11-10
• [Presentation] RNAの識別と管理-自己、異常な自己、そして非自己- (2011)
  • Author(s): 稲田利文
  • Organizer: フォーラムinドージシ
  • Place of Presentation: 熊本、日本(招待講演)
  • Year and Date: 2011-11-04
• [Presentation] Dual function of Dom34:Hbs1 complex in mRNA clearance coupled with translation arrest in yeast (2011)
  • Author(s): Toshifumi Inada
  • Organizer: Protein Synthesis and Translational Control Meeting
  • Place of Presentation: EMBL, Heidelberg, Germany
  • Year and Date: 2011-09-08
• [Presentation] The novel roles of Dom34:Hbs1 complex in No-Go-Decay quality control systems in yeast (2011)
  • Author(s): Tatsuhisa Tsuboi, Isao Kashima, Kazushige Kuroha, Shiho Makino, Kazuhei Kudo, Toshifumi Inada
  • Organizer: mRNA processing Meeting
  • Place of Presentation: CSHL, NY, USA
  • Year and Date: 2011-08-24
• [Presentation] Dual functions of Dom34:Hbs1 complex in mRNA clearance coupled with translation arrest in yeast (2011)
  • Author(s): Toshifumi Inada
  • Organizer: RNA Society Meeting 2011
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2011-06-17
• [Presentation] 1W20翻訳の分子基盤からRNA品質管理機構の理解へ (2010)
  • Author(s): 稲田利文
  • Organizer: BMB2010(日本分子生物学会・日本生化学会、合同年会)
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-07
• [Presentation] 40S-ribosome-bound RACK1 functions in nascent peptide-dependent translation arrest that leads to Dom34/Hbs1-independent NGD (2010)
  • Author(s): Kazushige Kuroha, Lyudmila Dimitrova, Yuki Kato, Takehiko Itoh, Katsuhiko Shirahige, Toshifumi Inada
  • Organizer: Translational Control Meeting
  • Place of Presentation: CSHL, NY, USA
  • Year and Date: 2010-09-15
• [Presentation] Rack1 participates in nascent polypeptide-dependent translation arrest (2010)
  • Author(s): Kazushige Kuroha, Lyudmila Dimitrova, Yuki Kato, Takehiko Itoh, Katsuhiko Shirahige, Toshifumi Inada
  • Organizer: Ribosome 2010
  • Place of Presentation: Orvieto, Italy
  • Year and Date: 2010-05-15
• [Book] 実験医学増刊号『生命分子を統合するRNA』31 (2013)
  • Author(s): 塩見春彦、稲田利文、泊幸秀、廣瀬哲郎編
  • Publisher: 羊土社
• [Book] 「RNA品質管理機構における停滞リボソーム解離複合体の普遍的機能」生化学84 (2012)
  • Author(s): 稲田利文
  • Publisher: 日本生化学学会
• [Book] 「RNA品質管理機構とあらたな遺伝子疾患治療薬の開発」医学のあゆみ『RNA医学-医療-あらたな診断-治療を拓く』238 (2011)
  • Author(s): 稲田利文
  • Publisher: 医歯薬出版株式会社
• [Book] 実験医学増刊号「拡大・進展を続けるRNA研究の最先端」28 (2010)
  • Author(s): 塩見春彦、塩見美喜子、稲田利文、廣瀬哲郎編
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.pharm.tohoku.ac.jp/~idenshi/inada_lab_HP/HOME(Japanese).html
• [Remarks] 遺伝子制御薬学分野HP
  • URL: http://www.pharm.tohoku.ac.jp/~idenshi/inada_lab_HP/HOME(Japanese).html
• [Remarks]
  • URL: http://www.pharm.tohoku.ac.jp/~idenshi/idenshi-j.shtml
• [Remarks]
  • URL: http://web.me.com/toshiinada/INADA_group_site/Welcome.html