Live observation of three dimensional hepatic tissue with microvascular networks.
| Project/Area Number |
22390260
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
KOIKE Naoto 横浜市立大学, 医学研究科, 客員研究員 (50301081)
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| Co-Investigator(Kenkyū-buntansha) |
TANIGUCHI Hideki 横浜市立大学, 医学研究科, 教授 (70292555)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 血管 / 肝臓 / 組織再構築 / 再生医療 / 再生医学 |
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| Research Abstract |
We provide a proof-of-concept study to engineer human liver tissue with fully functional vascular networks. We established a stable 3D culture platform to vascularize h uman fetal liver cells combined with human umbilical vein endothelial and mesenchymal cell populations. In vitro vascularized fetal liver cells were intravitally monitored the dynamic cellular organizing process after implantation under transparency window (cranial window). Engineered human tissue shows multiple liver-specific features with several hepatic functions. By analysis of the resected transplanted tissues and further animal experiments, it was confirmed that the tissues possessed the nature of the mature human hepatic tissue. Our new techniques discovered here should facilitate not only for future clinical use, but also for industrial use such as drug testing.
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] Vascularised and functional human liver tissue from an induced pluripotent stem cell-derived organ bud transplant2013
Author(s)
Takebe T, Sekine K, Enomura M, Koike H, Kimura M, Ogaeri T, Zang RR, Ueno Y, Zheng YW, Koike N, Aoyama S, Adachi Y, Taniguchi H
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Journal Title
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Peer Reviewed
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[Journal Article] Imaging studies of intraductal tubulopapillary neoplasms of the pancreas: 2-tone duct sign and cork-of-wine-bottle sign as indicators of intraductal tumor growth.2012
Author(s)
Motosugi U, Yamaguchi H, Furukawa T, Ichikawa T, Hatori T, Fujita I, Yamamoto M, Motoi F, Kanno A, Watanabe T, Koike N, Koyama I, Kobayashi J, Shimizu M
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Journal Title
J Comput Assist Tomogr
Volume: 36
Pages: 710-717
DOI
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Peer Reviewed
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[Presentation] Creation of long lasting human hepatic tissue in vivo2010
Author(s)
Naoto Koike, Ryoji Fujiwara, Akira Nakano, Toyoki Chiba, Takeru Amiya, Keisuke Sekine, Hideki Taniguchi
Organizer
25th Anniversary tumor course.Critical Issues in Tumor Microenvironment, Angiogenesis and Metastasis.
Place of Presentation
Harvard University, Cambridge, USA
Year and Date
2010-09-26
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