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Live observation of three dimensional hepatic tissue with microvascular networks.

Research Project

Project/Area Number 22390260
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionYokohama City University

Principal Investigator

KOIKE Naoto  横浜市立大学, 医学研究科, 客員研究員 (50301081)

Co-Investigator(Kenkyū-buntansha) TANIGUCHI Hideki  横浜市立大学, 医学研究科, 教授 (70292555)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords血管 / 肝臓 / 組織再構築 / 再生医療 / 再生医学
Research Abstract

We provide a proof-of-concept study to engineer human liver tissue with fully functional vascular networks. We established a stable 3D culture platform to vascularize h uman fetal liver cells combined with human umbilical vein endothelial and mesenchymal cell populations. In vitro vascularized fetal liver cells were intravitally monitored the dynamic cellular organizing process after implantation under transparency window (cranial window). Engineered human tissue shows multiple liver-specific features with several hepatic functions. By analysis of the resected transplanted tissues and further animal experiments, it was confirmed that the tissues possessed the nature of the mature human hepatic tissue. Our new techniques discovered here should facilitate not only for future clinical use, but also for industrial use such as drug testing.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (50 results)

All 2013 2012 2011 2010

All Journal Article (28 results) (of which Peer Reviewed: 18 results) Presentation (22 results) (of which Invited: 2 results)

  • [Journal Article] Vascularised and functional human liver tissue from an induced pluripotent stem cell-derived organ bud transplant2013

    • Author(s)
      Takebe T, Sekine K, Enomura M, Koike H, Kimura M, Ogaeri T, Zang RR, Ueno Y, Zheng YW, Koike N, Aoyama S, Adachi Y, Taniguchi H
    • Journal Title

      Nature

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The generation of pancreatic β-cell spheroids in a simulated microgravity culture system.2013

    • Author(s)
      Tanaka H
    • Journal Title

      Biomaterials

      Volume: 34 Issue: 23 Pages: 5785-5791

    • DOI

      10.1016/j.biomaterials.2013.04.003

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vascularised and functional human liver tissue from an induced pluripotent stem cell-derived organ bud transplant2013

    • Author(s)
      Takanori Takebe
    • Journal Title

      Nature

      Volume: in press

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] FOLFOX療法後の脾腫に伴う血小板減少に対し腹腔鏡下脾摘術が奏効した進行直腸癌の1例2013

    • Author(s)
      小池直人
    • Journal Title

      癌と化学療法

      Volume: in press

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of functional human vascular network in vitro2012

    • Author(s)
      Takebe T
    • Journal Title

      Transplant Proc

      Volume: 44(4) Issue: 4 Pages: 1130-1133

    • DOI

      10.1016/j.transproceed.2012.03.039

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Imaging Studies of Intraductal Tubulopapillary Neoplasms of the Pancreas: 2-Tone Duct Sign and Cork-of-Wine-Bottle Sign as Indicators of Intraductal Tumor Growth2012

    • Author(s)
      Motosugi U, Yamaguchi H, Furukawa T, Ichikawa T, Hatori T, Fujita I, Yamamoto M, Motoi F, Kanno A, Watanabe T, Koike N, Koyama I, Kobayashi J, Shimizu M
    • Journal Title

      J Comput Assist Tomogr

      Volume: 36 Issue: 6 Pages: 710-717

    • DOI

      10.1097/rct.0b013e31826d1fc8

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gd-EOB-DTPA-Enhanced MRI versus Extracellular Contrast Medium-Enhanced MRI in Differentiation of Metastatic from Benign Liver Lesions2012

    • Author(s)
      Koike N, Onaya H
    • Journal Title

      Ann Gastroenterol Hepatol

      Volume: 3 Pages: 57-62

    • URL

      http://www.agh.sanlucasmedical.com/Issue/1-2012

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel concept of identifying precancerous cells to enhance anti-cancer therapies2012

    • Author(s)
      Zheng YW, Tsuchida T, Taniguchi H
    • Journal Title

      J Hepatobiliary Pancreat Sci

      Volume: 19 Issue: 6 Pages: 621-625

    • DOI

      10.1007/s00534-012-0546-2

    • NAID

      10031126977

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Self-organization of human hepatic organoid by recapitulating organogenesis in vitro2012

    • Author(s)
      Takebe T
    • Journal Title

      Transplant Pro

      Volume: 44(4) Issue: 4 Pages: 1018-1020

    • DOI

      10.1016/j.transproceed.2012.02.007

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Basics and applications of stem cells in the pancreas2012

    • Author(s)
      Sekine K, Taniguchi H
    • Journal Title

      J Hepatobiliary Pancreat Sci

      Volume: 19 Issue: 6 Pages: 594-599

    • DOI

      10.1007/s00534-012-0545-3

    • NAID

      10031126973

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] Characteristics of hepatic stem/progenitor cells in the fetal and adult liver2012

    • Author(s)
      Koike H, Taniguchi H
    • Journal Title

      J Hepatobiliary Pancreat Sci

      Volume: 19 Issue: 6 Pages: 587-593

    • DOI

      10.1007/s00534-012-0544-4

    • NAID

      10031126972

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] Establishment of Automated Culture System for Murine Induced Pluripotent Stem Cells2012

    • Author(s)
      Koike H
    • Journal Title

      BMC Biotechnology

      Volume: 12 Issue: 1 Pages: 81-81

    • DOI

      10.1186/1472-6750-12-81

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stem Cell、iPS研究;再生医療、癌診療への展開】 総論 肝臓領域における幹細胞研究の動向2012

    • Author(s)
      谷口英樹
    • Journal Title

      肝・胆・膵

      Volume: 65 Pages: 7-12

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 【消化器幹細胞研究の可能性】 肝幹細胞研究の最新動向2012

    • Author(s)
      谷口英樹
    • Journal Title

      G.I.Research

      Volume: 20 Pages: 30-36

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 肝幹細胞研究の最新動向2012

    • Author(s)
      谷口英樹, 内藤貴子, 上野康晴
    • Journal Title

      G.I.Research

      Volume: 20 Pages: 30-36

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Reconstitution of hepatic tissue architectures from fetal liver cells obtained from a three-dimensional culture with a rotating wall vessel bioreactor2011

    • Author(s)
      Ishikawa M, Sekine K, Okamura A, Zheng YW, Ueno Y, Koike N, Tanaka J, Taniguchi H
    • Journal Title

      J Biosci Bioeng

      Volume: 111 Issue: 6 Pages: 711-718

    • DOI

      10.1016/j.jbiosc.2011.01.019

    • NAID

      110008672821

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evidence for mesenchymal-epithelial transition associated with mouse hepatic stem cell differentiation2011

    • Author(s)
      Li B, Zheng YW, Sano Y, Taniguchi H
    • Journal Title

      PLoS One

      Volume: 6 Issue: 2 Pages: e17092-e17092

    • DOI

      10.1371/journal.pone.0017092

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reconstruction of human elastic cartilage by a CD44+ CD90+ stem cell in the ear perichondrium2011

    • Author(s)
      Kobayashi S, Takebe T, Inui M, Iwai S, Kan H, Zheng YW, Maegawa J, Taniguchi H
    • Journal Title

      ProcNatl Acad Sci U S A

      Volume: 108 Pages: 14479-14484

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 胆嚢炎と十二指腸狭窄を合併したsegmental arterial mediolysisの1例2011

    • Author(s)
      木原一樹、小池直人、大島祐二、武内俊章、有田誠司、渡橋和政
    • Journal Title

      日本臨床外科学会雑誌

      Volume: 72 Pages: 711-718

    • NAID

      10029897028

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 双孔式人工肛門脱を自動縫合器による局所治療にて修復した1例2011

    • Author(s)
      大島祐二、小池直人、武内俊章、有田誠司、大河内信弘
    • Journal Title

      手術

      Volume: 65 Pages: 655-657

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reconstruction of human elastic cartilage by a CD44+CD90+ stem cell in the ear perichondrium2011

    • Author(s)
      Kobayashi S, Takebe T, Inui M, Iwai S, Kan H, Zheng YW, Maegawa J, Taniguchi H
    • Journal Title

      Proc Natl A cad Sci USA

      Volume: 108 Issue: 35 Pages: 1479-1484

    • DOI

      10.1073/pnas.1109767108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] フローサイトメトリーを用いた膵幹/前駆細胞の特性解析2011

    • Author(s)
      谷口英樹
    • Journal Title

      胆と膵

      Volume: 32 Pages: 1207-1212

    • Related Report
      2011 Annual Research Report
  • [Journal Article] 固形腫瘍幹細胞膵がんにおけるがん幹細胞の特性解析2011

    • Author(s)
      谷口英樹, 小池博之
    • Journal Title

      実験医学

      Volume: 29 Pages: 3254-3261

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Reconstitution of hepatic tissue architectures from fetal liver cells obtained from a three-dimensional culture with a rotating wall vessel bioreactor.2011

    • Author(s)
      Ishikawa M, Sekine K, Okamura A, Zheng YW, Ueno Y, Koike N, Tanaka J, Taniguchi H.
    • Journal Title

      J Biosci Bioeng.

      Volume: (Epub ahead of print)

    • NAID

      110008672821

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evidence for mesenchymal-epithelial transition associated with mouse hepatic stem cell differentiation.2011

    • Author(s)
      Li B, Zheng YW, Sano Y, Taniguchi H.
    • Journal Title

      PLoS One.

      Volume: 6(2)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ヒト肝幹細胞ライブラリーの構築によるファーマコセロミクスの開発2011

    • Author(s)
      谷口英樹
    • Journal Title

      医薬品医療機器レギュラトリーサイエンス

      Volume: 42(3) Pages: 185-190

    • NAID

      40018756248

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 幹細胞の臨床応用 臨床応用に向けた幹細胞操作法開発における現状と課題2011

    • Author(s)
      谷口英樹
    • Journal Title

      Frontiers in Gastroenterology

      Volume: 16(1) Pages: 62-71

    • Related Report
      2010 Annual Research Report
  • [Journal Article] フローサイトメトリーを用いた膵管前駆細胞の選択的分離法2010

    • Author(s)
      谷口英樹
    • Journal Title

      Surgery Frontier

      Volume: 17(2) Pages: 162-165

    • Related Report
      2010 Annual Research Report
  • [Presentation] 如何にして器官発生を模倣するのか?ー血管系を有するヒト肝組織創出法の開発ー2013

    • Author(s)
      谷口英樹
    • Organizer
      第12回日本再生医療学会総会(招待講演)
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2013-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト微小血管網を有する膵島様組織の創出2013

    • Author(s)
      江野村允宏
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 如何にして器官発生を模倣するのか?ー血管系を有するヒト肝組織創出法の開発ー2013

    • Author(s)
      谷口英樹
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 当院における超高齢者の肝胆膵悪性腫瘍に対する手術の検討2012

    • Author(s)
      小池直人
    • Organizer
      第67回日本消化器外科学会総会
    • Place of Presentation
      富山県民会館(富山県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 当院における大腸癌肝転移に対する肝切除例の検討2012

    • Author(s)
      小池直人
    • Organizer
      第67回大腸肛門病学会学術集会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト微小血管網を有する膵島様組織の創出2012

    • Author(s)
      高橋禎暢
    • Organizer
      第39回日本臓器保存生物医学会学術集会
    • Place of Presentation
      コラッセふくしま(福島県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 多能性幹細胞を用いた機能的なヒト臓器の創出2012

    • Author(s)
      武部貴則
    • Organizer
      第39回日本臓器保存生物医学会学術集会
    • Place of Presentation
      コラッセふくしま(福島県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 術前診断に苦慮した膵Acinar cell carcinomaの1切除例2012

    • Author(s)
      磯崎哲朗
    • Organizer
      千葉肝胆膵外科フォーラム
    • Place of Presentation
      三井ガーデンホテル千葉(千葉県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Our treatment strategies for liver metastases from colorectal cancers2012

    • Author(s)
      Naoto Koike
    • Organizer
      IASGO 2012
    • Place of Presentation
      Shangri-la Hotel (Bangkok, Thailand)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] In vitroでの自己組織化肝オルガノイド形成2011

    • Author(s)
      武部貴則, 関根圭輔, 江野村允宏, 上野康晴, 鄭允文, 谷口英樹
    • Organizer
      第47回日本移植学会
    • Place of Presentation
      仙台国際センター宮城県
    • Year and Date
      2011-10-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] 消化管における幹細胞研究の現状と展望2011

    • Author(s)
      谷口英樹
    • Organizer
      第47回日本移植学会
    • Place of Presentation
      仙台国際センター宮城県(招待講演)
    • Year and Date
      2011-10-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Evaluation of drainage fluid amylase level after pancreatojejunostomy using our method in pancreatoduodenectomy2011

    • Author(s)
      Koike N, Ohshima Y, Arita S, Takeuchi T, Ohkohchi N
    • Organizer
      International Surgical Week
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-08-31
    • Related Report
      2012 Final Research Report
  • [Presentation] Evaluation of drainage fluid amylase level after pancreatojejunostomy using our method inpancreatoduodenectomy2011

    • Author(s)
      Koike N, Ohshima Y, Arita S、Takeuchi T, Ohkohchi N
    • Organizer
      International Surgical Week 2011
    • Place of Presentation
      パシフィコ横浜神奈川県
    • Year and Date
      2011-08-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] 当科での膵空腸吻合法と術後排液量、排液アミラーゼ値の検討2011

    • Author(s)
      小池直人、大島祐二、武内俊章、大河内信弘
    • Organizer
      第66回日本消化器外科学会総会
    • Place of Presentation
      名古屋国際会議場愛知県
    • Year and Date
      2011-07-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] 十二指腸乳頭部癌に対する弾頭兼十二指腸第II部切除術2011

    • Author(s)
      小池直人、大島祐二、大河内信弘
    • Organizer
      第23回日本肝胆膵外科学会・学術集会
    • Place of Presentation
      京王プラザホテル東京
    • Year and Date
      2011-06-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] 胃癌診断治療における組織型分類の意義2011

    • Author(s)
      小池直人
    • Organizer
      佐倉四街道地区消化器病検討会
    • Place of Presentation
      佐倉市健康管理センター(千葉)(招待講演)
    • Year and Date
      2011-02-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 幹細胞から癌を読み解く 癌幹細胞という概念2010

    • Author(s)
      谷口英樹
    • Organizer
      第49回日本婦人科腫瘍学会学術講演会
    • Place of Presentation
      佐賀文化会館(佐賀)(招待講演)
    • Year and Date
      2010-12-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] 再生医療を応用したがん治療 肝前駆細胞の増殖制御に基づく肝発癌抑制療法の開発2010

    • Author(s)
      谷口英樹
    • Organizer
      第48回日本癌治療学会学術集会
    • Place of Presentation
      京都国際会議場(京都)(招待講演)
    • Year and Date
      2010-10-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Creation of long lasting human hepatic tissue in vivo2010

    • Author(s)
      Koike N, Fujiwara R, Nakano A, Chiba T, Amiya T, Sekine K, Taniguchi H
    • Organizer
      25thAnniversary tumor course. Critical issues in tumor microenvironment, angiogenesis and metastasis
    • Place of Presentation
      Harvard University, Cambridge, USA
    • Year and Date
      2010-09-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Creation of long lasting human hepatic tissue in vivo2010

    • Author(s)
      Naoto Koike, Ryoji Fujiwara, Akira Nakano, Toyoki Chiba, Takeru Amiya, Keisuke Sekine, Hideki Taniguchi
    • Organizer
      25th Anniversary tumor course.Critical Issues in Tumor Microenvironment, Angiogenesis and Metastasis.
    • Place of Presentation
      Harvard University, Cambridge, USA
    • Year and Date
      2010-09-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 障害肝合併例における脾摘・部分的脾塞栓療法の意義2010

    • Author(s)
      小池直人、大島祐二、武内俊章、有田誠司
    • Organizer
      第65回日本消化器外科学会総会
    • Place of Presentation
      海峡メッセ下関(山口)
    • Year and Date
      2010-07-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] 当科における膵空腸密着吻合法と術後排液アミラーゼ値の推移2010

    • Author(s)
      小池直人、大島祐二、武内俊章、有田誠司、大河内信弘
    • Organizer
      第22回日本肝胆膵外科学会・学術集会
    • Place of Presentation
      仙台国際センター(宮城)
    • Year and Date
      2010-05-26
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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