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Development of regenerative medicine using human iPS cellsFor pulp and periapical pathoses

Research Project

Project/Area Number 22390360
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionNagasaki University

Principal Investigator

HAYASHI Yoshihiko  長崎大学, 大学院・医歯薬学総合研究科, 教授 (20150477)

Co-Investigator(Kenkyū-buntansha) YAMADA Shizuka  長崎大学, 大学院・医歯薬学総合研究科, 准教授 (00363458)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywords再生医療 / iPS細胞 / 低酸素培養 / 細胞増殖・分化 / 骨芽細胞誘導
Research Abstract

There are no stastical differences in either the growth and differentiation of (induced pluripotent stem) iPS cells or the induction of iPS cells to osteoblasts between the four and three transcription factor froups cultured under hypoxia. Furthermore, the function of (hypoxia inducible factors) HIFs in murine iPS cells under hypoxia was investigated in relation to the morphology and expression of the transcription factors. The HIF-2α knockdown group exhibited a decreased colony size in the iPS cells. The HIF-2α or -3α demonstrated a statistical significant decrease in the expression of the transcription factors compared to those observed in the control group. These results demonstrate that HIF-2α among HIFs is the most influential candidate for the maintenance of the pluripotency of murine iPS cells.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report

Research Products

(30 results)

All 2013 2012 2011 2010 Other

All Journal Article Presentation Book

  • [Journal Article] Effects of glucosamine on the tooth pulpal nociceptive responses in the rat2013

    • Author(s)
      Kaida K, Yamashita H, Toda K, Hayashi Y.
    • Journal Title

      Journal of Dental Sciences

      Volume: 8 Pages: 68-73

    • Related Report
      2012 Final Research Report
  • [Journal Article] Effects of fish collagen peptides on collagen post-translational modifications and mineralization in an osteoblastic cell culture system2013

    • Author(s)
      Yamada S, Nagaoka H, Terajima M, Tsuda N, Hayashi Y, Yamauchi M.
    • Journal Title

      Dental Materials Journal

      Volume: 32 Pages: 1-8

    • NAID

      10031138706

    • Related Report
      2012 Final Research Report
  • [Journal Article] Challenges to the clinical placement and evaluation of adhesively-bonded, cervical composite restorations2013

    • Author(s)
      Kubo S, Yokota H, Yokota H, Hayashi Y.
    • Journal Title

      Dent Mater

      Volume: 29(1) Pages: 10-27

    • NAID

      120005438907

    • Related Report
      2012 Final Research Report
  • [Journal Article] Early gene and protein expression associated with osteoblast proliferation and differentiation in response to fish collagen peptides2013

    • Author(s)
      Yamada S, Yoshizawa Y, Kawakubo A, Ikeda T, Yanagiguchi K, Hayashi Y
    • Journal Title

      Dental Materials Journal

      Volume: 32 Pages: 233-240

    • Related Report
      2012 Final Research Report
  • [Journal Article] Effects of glucosamine on the tooth pulpal nociceptive responses in the rat.2013

    • Author(s)
      Kaida K
    • Journal Title

      Journal of Dental Sciences

      Volume: 8 Pages: 68-73

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of fish collagen peptides on collagen post-translational modifications and mineralization in an osteoblastic cell culture system.2013

    • Author(s)
      Yamada S
    • Journal Title

      Dental Materials Journal

      Volume: 32 Pages: 1-8

    • NAID

      10031138706

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Challenges to the clinical placement and evaluation of adhesively-bonded, cervical composite restorations.2013

    • Author(s)
      Kubo S
    • Journal Title

      Dent Materials

      Volume: 29 Pages: 10-27

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Early gene and protein expression associated with osteoblast proliferation and differentiation in response to fish collagen peptides.2013

    • Author(s)
      Yamada S
    • Journal Title

      Dental Materials Journal

      Volume: 32 Pages: 233-240

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation2012

    • Author(s)
      Kawasaki A, Hayashi Y, Yanagiguchi K, Yamada S, Syudo M, Igawa K, Ikeda T, Kubo S, Fujiwara M.
    • Journal Title

      Journal of dental Sciences

      Volume: 7 Pages: 94-98

    • NAID

      120004754249

    • Related Report
      2012 Final Research Report
  • [Journal Article] Effects of eluted components from 4-META/MMA-TBB adhesive resin sealer on osteoblastic cell proliferation.2012

    • Author(s)
      Kawasaki A
    • Journal Title

      Journal of Dental Sciences

      Volume: 7 Pages: 94-98

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Zinc as as essential trace element in the acceleration of matrix vesicles-mediated mineral deposition2011

    • Author(s)
      Kawakubo A, Matsunaga T, Ishizaki H, Yamada S, Hayashi Y.
    • Journal Title

      Microscopy research and techniques

      Volume: 74 Pages: 1161-1165

    • Related Report
      2012 Final Research Report
  • [Journal Article] 根管充填用レジンシーラーの生体親和性2011

    • Author(s)
      柳口嘉治郎、首藤 実、川崎 綾、杉本浩司、池田 毅、山田志津香、林 善彦
    • Journal Title

      本歯内療法学会雑誌

      Volume: 32(3) Pages: 212-216

    • NAID

      10029697632

    • Related Report
      2012 Final Research Report
  • [Presentation] マウスiPS 細胞の低酸素下培養におけるHIF(Hypoxia Inducible factor) の役割2012

    • Author(s)
      杉本浩司,吉澤 有,石崎秀隆,林 善彦
    • Organizer
      第136回日本歯科保存学会春季学術大会
    • Place of Presentation
      宜野湾市
    • Related Report
      2012 Final Research Report
  • [Presentation] 歯髄再生に使用する足場材2012

    • Author(s)
      林 善彦
    • Organizer
      第10回日本再生歯科医学会
    • Place of Presentation
      神戸市
    • Related Report
      2012 Final Research Report
  • [Presentation] 低酸素下におけるiPS細胞の増殖・分化挙動2011

    • Author(s)
      杉本浩司, 石崎秀隆, 池田毅, 林善彦
    • Organizer
      第32回日本歯内療法学会学術大会
    • Place of Presentation
      長崎市
    • Year and Date
      2011-07-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] 因子・4因子導入マウスiPS細胞の低酸素培養における増殖・分化について2011

    • Author(s)
      杉本浩司, 石崎秀隆, 林善彦
    • Organizer
      第134回日本歯科保存学会春季大会
    • Place of Presentation
      浦安市
    • Year and Date
      2011-06-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] 3因子・4因子導入マウスiPS細胞の低酸素培養における増殖・分化について2011

    • Author(s)
      杉本浩司,石崎秀隆,林 善彦
    • Organizer
      第134回日本歯科保存学会春季学術大会
    • Place of Presentation
      浦安市
    • Related Report
      2012 Final Research Report
  • [Presentation] 低酸素下におけるPS細胞の増殖・分化挙動2011

    • Author(s)
      杉本浩司,石崎秀隆,池田 毅、林 善彦
    • Organizer
      第32回日本歯内療法学会学術大会
    • Place of Presentation
      長崎市
    • Related Report
      2012 Final Research Report
  • [Presentation] マウスiPS細胞の低酸素下培養におけるHIF(Hypoxia Inducible factor) の役割

    • Author(s)
      林 善彦
    • Organizer
      第136回日本歯科保存学会春季学術大会
    • Place of Presentation
      宜野湾市
    • Related Report
      2012 Annual Research Report
  • [Presentation] 歯髄再生に使用する足場材

    • Author(s)
      林 善彦
    • Organizer
      第10回日本再生歯科医学会
    • Place of Presentation
      神戸市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] Vhapter 16 Chitosan Application in Dentistry. In Marine Nutraceuticals: Prospects and perspective2013

    • Author(s)
      Hayashi Y, Yanagiguchi K, Koyama Z, Ikeda T, Yamada S
    • Related Report
      2012 Final Research Report
  • [Book] Marine Nutraceuticals: Prospects and Perapectives2013

    • Author(s)
      Hayashi Y
    • Total Pages
      444
    • Publisher
      CRC Press
    • Related Report
      2012 Annual Research Report
  • [Book] Chapter 6 Chitosan and fish collagen as biomaterials for regenerative medicine. In Marine Medical Food, Volume 65, AFNR2012

    • Author(s)
      Hayashi Y, Yamada S, Yanagiguchi K, Koyama Z, Ikeda T.
    • Publisher
      ed. Se-Kwon Kim, UK: Academic Press
    • Related Report
      2012 Final Research Report
  • [Book] Advances in Food and Nutrition Research Volume 652012

    • Author(s)
      Hayashi Y
    • Total Pages
      523
    • Publisher
      Academic Press
    • Related Report
      2012 Annual Research Report
  • [Book] Marine Cosmeceuticals: Trends and Prospects2012

    • Author(s)
      Hayashi Y
    • Total Pages
      412
    • Publisher
      CRC Press
    • Related Report
      2012 Annual Research Report
  • [Book] Marine Medical Food, Volume 65, Advances in Food and Nutrition Research2012

    • Author(s)
      Hayashi Y, Yamada S, Yanagiguchi K, Koyama Z, Ikeda T(分担)
    • Total Pages
      523
    • Publisher
      Academic Press
    • Related Report
      2011 Annual Research Report
  • [Book] Fish collagen and tissue repair. In "Marine Cosmeceuticals: Trends and Prospects"2011

    • Author(s)
      Hayashi Y, Yamada S, Ikeda T, Yanagiguchi K.
    • Publisher
      ed. S-K. Kim, CRC Press, Boca Raton, FL
    • Related Report
      2012 Final Research Report
  • [Book] Marine Cosmeceuticals : Trends and Prospects2011

    • Author(s)
      Hayashi Y, Yamada S, Ikeda T, Yanagiguchi K(分担)
    • Total Pages
      412
    • Publisher
      CRC Press
    • Related Report
      2011 Annual Research Report
  • [Book] Chitin, Chitosan, Oligosaccharides and Their Derivatives分担2011

    • Author(s)
      Yoshihiko Hayashi
    • Publisher
      CRC Press (Taylor & Francis Group)
    • Related Report
      2010 Annual Research Report
  • [Book] Application of chiosan oligosaccharide and glucosamine in dentistry. In Chitin, Chitosan, Oligosaccharides and Their Derivatives: Biological Activities and Applications2010

    • Author(s)
      Hayashi Y.
    • Publisher
      Se-Kwon Kim, CRC Press-Taylor & Francis Group
    • Related Report
      2012 Final Research Report

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Published: 2010-08-22   Modified: 2019-07-29  

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