The control strategy of stress-induced UCP2 expression and ROS generation in diabetes mellitus

• Project/Area Number: 22500767
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Kyoto Prefectural University
• Principal Investigator: MINAMIYAMA Yukiko 京都府立大学, 大学院・生命環境科学研究科, 教授 (00362989)
• Co-Investigator(Kenkyū-buntansha): ICHIKAWA Hiroshi 同志社大学, 生命医科学部, 教授 (60336732) ; TAKEMURA Shigekazu 大阪市立大学, 医学研究科, 講師 (00322363)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 生活習慣病 / ミトコンドリア機能 / 低鉄 / 抗酸化物質 / 食事制限 / UCP2 / ミトコンドリア機能障害 / 二型糖尿病 / 酸化ストレス

Research Abstract

It was found that the enhancement of oxidative stress by mitochondrial dysfunction and over-expression of UCP2 that negatively regulates reactive oxygen species (ROS) from mitochondria in diabetes mellitus.Calorie restrictions improved the diabetic complications.Furthermore, we found that iron deposition due to abnormal iron metabolism in diabetic tissues.It has been clear that the removal of iron therapy is effective in the treatment of diabetes. Thus, phlebotomy treatments currently being conducted in chronic hepatitis C treatment can be expected as an effective treatmentas an applicationto diabetes, in developing countries, particularly. In addition, antioxidants are also effective. S-allyl cysteine that is an antioxidantcomponent of the aged garliccould improve diabetic condition by controlling the mechanisms related to lipid metabolism and glucose metabolism.

Research Products

• [Journal Article] Comparison of in vivo and in vitro antioxidative parameters for eleven food factors (2013)
  • Author(s): HP. Indo, I. Nakanishi, K. Ohkubo, Hsiu-Yen. Chuan, M. Nyui, S. Manda, K. Matsumoto, K. Fukuhara, K. Anzai, N. Ikota, H. Matsui, Y. Minamiyama, A. Nakajima, H. Ichikawa, S. Fukuzumi, T. Ozawa, C. Mukai, and HJ. Majima
  • Journal Title: The Royal Society of Chemistry Volume: 3 Pages: 4535-4538
  • Peer Reviewed
• [Journal Article] S-allyl cysteine improves nonalcoholic fatty liver disease in type 2 diabetes Otsuka Long-Evans Tokushima Fatty rats via regulation of hepatic lipogenesis and glucose metabolism (2013)
  • Author(s): Takemura S, Minamiyama Y, Kodai S, Shinkawa H, Tsukioka T, Okada S, Azuma H, Kubo S.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: in press
  • NAID: 130004466717
  • Peer Reviewed
• [Journal Article] Suppression of indomethacin-induced apoptosis in the small intestine due to Bach1 deficiency (2011)
  • Author(s): A. Harusato, Y. Naito, T. Takagi, K. Uchiyama, K. Mizushima, Y. Hirai, S. Yamada, T. Tuji, H. Yoriki, R. Horie, K. Inoue, K. Fukumoto, O. Handa, T. Ishikawa, S. Kokura, Y. Minamiyama,H. Ichikawa, A. Muto, K. Igarashi, T. Yoshikawa.
  • Journal Title: Free Radic Res Volume: 45(6) Pages: 717-27
  • Peer Reviewed
• [Journal Article] Suppression of indomethacin-induced apoptosis in the small intestine due to Bach1 deficiency (2011)
  • Author(s): Harusato A, Naito Y, Takagi T, Uchiyama K, Mizushima K, Hirai Y, Yamada S, Tuji T, Yoriki H, Horie R, Inoue K, Fukumoto K, Handa O, Ishikawa T, Kokura S, Minamiyama Y, Ichikawa H, Muto A, Igarashi K, Yoshikawa T
  • Journal Title: Free Radic Res Volume: 45(6) Issue: 6 Pages: 717-727
  • DOI: 10.3109/10715762.2011.574287
  • Peer Reviewed
• [Journal Article] 小腸上皮損傷治癒における各種アミノ酸の役割 (2011)
  • Author(s): 高木智久、内藤裕二、岸本悦子、足立聡子、水島かつら、林奈津子、南山幸子、市川寛、内山和彦、半田修、石川剛、吉田直久、小西英幸、若林直樹、八木信明、古倉聡、吉川敏一
  • Journal Title: THE GI FOREFRONT Volume: 6 Pages: 186-186
• [Journal Article] 腸管炎症病態におけるBach1の役割 (2011)
  • Author(s): 春里暁人、内藤裕二、高木智久、内山和彦、辻俊史、水島かつら、石川剛、半田修、南山幸子、古倉聡、市川寛、武藤哲彦、五十嵐和彦、吉川敏一
  • Journal Title: G.I.Research Volume: 19 Pages: 398-398
• [Journal Article] Iron restriction improves type II diabetes mellitus of Otsuka Long-Evans Tokushima Fatty (OLETF) rats via inhibition of endogenously reactive oxygen species and the regulation of pancreas peroxisome proliferator-activated receptor-delta (PPAR-delta) and hypoxia inducible factor (HIF)-1alpha. (2010)
  • Author(s): Y. Minamiyama,S. Takemura, S. Kodai, H. Shinkawa, T. Tsukioka, Y. Naito, H. Ichikawa, T. Yoshikawa, S. Okada
  • Journal Title: Am J Physiol Volume: 298(6)
  • Peer Reviewed
• [Journal Article] Coadministration of carvedilol attenuates nitrate tolerance by preventing cytochrome p450 depletion. (2010)
  • Author(s): A. Nakahira, Y. Minamiyama, S. Takemura,H. Hirai, Y. Sasaki, S. Okada, Y. Funae, S. Suehiro
  • Journal Title: Circ J Volume: Aug;74(8) Pages: 1711-7
  • NAID: 10026566100
  • Peer Reviewed
• [Journal Article] Generation of reactive oxygen species in sperms of rats as an earlier marker for evaluating the toxicity of endocrine-disrupting chemicals. (2010)
  • Author(s): Y. Minamiyama, H. Ichikawa, S. Takemura, H. Kusunoki, Y. Naito, T. Yoshikawa
  • Journal Title: Free Radic Res Volume: 44(12) Pages: 1398-406
  • Peer Reviewed
• [Journal Article] Generation of reactive oxygen species in sperms of rats as an earlier marker for evaluating the toxicity of endocrine-disrupting chemicals. (2010)
  • Author(s): Minamiyama Y, Ichikawa H, Takemura S, Kusunoki H, Naito Y, Yoshikawa T.
  • Journal Title: Free Radic Res. Volume: 44 Pages: 1398-1406
  • Peer Reviewed
• [Journal Article] Coadministration of carvedilol attenuates nitrate tolerance by preventing cytochrome p450 depletion. (2010)
  • Author(s): Nakahira A, Minamiyama Y, Takemura S, Hirai H, Sasaki Y, Okada S, Funae Y, Suehiro S
  • Journal Title: Circ J. Volume: 74 Pages: 1711-1717
  • NAID: 10026566100
  • Peer Reviewed
• [Journal Article] S-allyl cysteine improves nonalcoholic fatty liver disease in type 2 diabetes Otsuka Long-Evans Tokushima Fatty rats via regulation of hepatic lipogenesis and glucose metabolism
  • Author(s): S. Takemura, Y. Minamiyama, S. Kodai, H. Shinkawa, T. Tsukioka, S. Okada, H. Azuma, S. Kubo
  • Journal Title: J. Clin. Biol. Nutr. Volume: (in press)
  • NAID: 130004466717
  • Peer Reviewed
• [Presentation] Effects of Iron Restriction on Hepatic Mitochondrial Iron Metabolism and Oxidative Stress in Nonalcoholic Fatty Liver Disease (NAFLD) in Otsuka Long-Evans Tokushima Fatty (OLETF) Rats (2013)
  • Author(s): Y. Minamiyama, S. Takemura, S. Taketani, Y. Naito, H. Ichikawa,T. Takagi, S. Okada, and T. Yoshikawa
  • Organizer: Digestive Disease Week 2013
  • Place of Presentation: Orland, FL
• [Presentation] Attenuation of nonalcoholic fatty liver disease (NAFLD) in type 2 diabetes Otsuka Long-Evans Tokushima Fatty (OLETF) rats via regulation of hepatic lipogenesis and glucose metabolism by administration of S-allyl cysteine (2013)
  • Author(s): Takemura S, Minamiyama Y, Kodai S, Shinkawa H, Kubo S
  • Organizer: Digestive Disease Week 2013
  • Place of Presentation: Orland, FL
• [Presentation] Effects of Iron Restriction on Hepatic Mitochondrial Iron Metabolism and Oxidative Stress in Nonalcoholic Fatty Liver Disease (NAFLD) in Otsuka Long-Evans Tokushima Fatty (OLETF) Rats (2013)
  • Author(s): Minamiyama Y, Takemura S, et al.
  • Organizer: DDW2013
  • Place of Presentation: Orland, USA
• [Presentation] Attenuation of nonalcoholic fatty liver disease (NAFLD) in type 2 diabetes Otsuka Long-Evans Tokushima Fatty (OLETF) rats via regulation of hepatic lipogenesis and glucose metabolism by administration of S-allyl cysteine (2013)
  • Author(s): Takemura S, Minamiyama Y, et al.
  • Organizer: DDW2013
  • Place of Presentation: Orland, USA
• [Presentation] Generation of Reactive Oxygen Species from the Rat Sperm Mitochondria is an Excellent Marker for the Biological Toxicity of Endocrine-Disrupting Chemicals (2012)
  • Author(s): H. Ichikawa, Y. Minamiyama, T. Takagi, O. Handa, Y. Naito, T. Yoshikawa
  • Organizer: 16th Biennial Meeting of the Society for Free Radical Research (International SFRR 2012)
  • Place of Presentation: London, UK
• [Presentation] Iron Metabolism of Hepatic Mitochondria and Oxidative Stress in Type 2 Diabetes Mellitus in Otsuka Long-Evans Tokushima Fatty (OLETF) Rats (Effect of Iron Depletion) (2012)
  • Author(s): Y. Minamiyama, S. Takemura, S. Taketani, Y. Naito, H. Ichikawa, K. Misumi. S. Okada, and T. Yoshikawa
  • Organizer: 16th Biennial Meeting of the Society for Free Radical Research (International SFRR 2012)
  • Place of Presentation: London, UK
• [Presentation] 除鉄療法は２型糖尿病モデルラットの治療に有効である (2012)
  • Author(s): 南山幸子、竹村茂一、市川 寛、内藤裕二、竹谷 茂、吉川敏一
  • Organizer: 第６6回日本栄養食糧学会大会
  • Place of Presentation: 東北大学（仙台）
• [Presentation] Iron Metabolism of Hepatic Mitochondria and Oxidative Stress in Type 2 Diabetes Mellitus in Otsuka Long-Evans Tokushima Fatty (OLETF) Rats (Effect of Iron Depletion) (2012)
  • Author(s): Minamiyama Y, Takemura S, et al.
  • Organizer: 16th Biennial Meeting of SFFRI
  • Place of Presentation: United Kingdom
• [Presentation] Effects of alpha-tocopherol and gamma-tocopherol on diabetic complications in type II diabetic rats (2011)
  • Author(s): Minamiyama Y, Takemura S, Ichikawa H, Okada S, Naito Y, Yoshikawa T
  • Organizer: International Conference on Food Factors 2011
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2011-11-21
• [Presentation] The role of histidine on wound healing using cultured rat intestinal epithelial cells (2011)
  • Author(s): Hori K, Ichikawa H, Takagi T, Minamiyama Y, Nakamura M, Doi A, Kawabata Y, Kaneda T, Uchiyama K, Naito Y, Yoshikawa T
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 2011-09-02
• [Presentation] The histidine affects delayed wound healing induced by indomethacin using cultured rat epithelial cells (2011)
  • Author(s): Matsuo S, Ichikawa H, Minamiyama Y, Takagi T, Fuchiue R, Emoto A, Suetsugu M, Uchiyama K, Naito Y, Yoshikawa T
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 2011-09-02
• [Presentation] The effect of Kampo Medicine "Dai-kenchu-to" on wound healing of rat intestinal epithelial cells (2011)
  • Author(s): Asai H, Ichikawa H, Minamiyama Y, Takagi T, Wada A, Ozaki Y, Okazaki K, Minami C, Handa O, Naito Y, Yoshikawa T
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 2011-09-02
• [Presentation] Sperm dysfunction from aging is involved in the oxidative stress (2011)
  • Author(s): Yamamoto R, Minamiyama Y, Ichikawa H, Takagi T, Ishii A, Sato Y, Miyanishi E, Yasui M, Handa O, Naito Y, Yoshikawa T
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 2011-09-02
• [Presentation] Generation of reactive oxygen species from the sperm mitochondria of rats is an excellent marker for the biological toxicity of endocrine-disrupting chemicals (2011)
  • Author(s): Miyanishi E, Minamiyama Y, Ichikawa H, Takagi T, Ishii A, Handa O, Naito Y, Yoshikawa T
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 2011-09-02
• [Presentation] Protective effect of polaprezinc on acetylsalicylic acid-induced apoptosis of rat intestinal epithelial cells-involvement of heat shock protein 70 and reactive oxygen species (2011)
  • Author(s): Handa O, Naito Y, Fukui A, Qin Y, Uehara Y, Tsuji T, Yoriki H, Horie R, Katada K, Kamada K, Uchiyama K, Ishikawa T, Takagi T, Minamiyama Y, Konishi H, Yagi N, Kokura S, Ichikawa H, Yoshikawa T
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 2011-09-01
• [Presentation] Calorie restriction improves diabetic complications via reduction of mitochondrial reactive oxygen species in Type II diabetic rats (2011)
  • Author(s): Minamiyama Y, Takemura S, Ichikawa H, Okada S, Yoshikawa T
  • Organizer: 4th INTERNATIONAL SYMPOSIUM NUTRITION, OXYGEN BIOLOGY, AND MEDICINE
  • Place of Presentation: Paris, France
  • Year and Date: 2011-06-16
• [Presentation] 2型糖尿病ラットにおける酸化ストレスと除鉄療法の効果 (2011)
  • Author(s): 南山幸子、竹村茂一、古川敏一
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-02
• [Presentation] 現代社会における安全・安心な食べ物～食性病害のない食品とは (2011)
  • Author(s): 南山幸子
  • Organizer: 第11回日本抗加齢医学会総会
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2011-05-28
• [Presentation] 加齢による精子機能と酸化ストレスに及ぼす熟成ニンニク成分(S-allyl cysteine)の効果について (2011)
  • Author(s): 南山幸子、山本隆平、竹村茂一、若原綾子、安井まどか、市川寛、吉川敏一
  • Organizer: 第65回日本栄養食糧学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-14
• [Presentation] Effects of alpha-tocopherol and gamma-tocopherol on diabetic complications in type II diabetic rats. (2011)
  • Author(s): Y. Minamiyama, S. Takemura, H. Ichikawa, S. Okada, Y. Naito and T. Yoshikawa
  • Organizer: International Conference on Food Factors 2011
  • Place of Presentation: Taipei, Taiwan
• [Presentation] The role of histidine on wound healing using cultured rat intestinal epithelial. (2011)
  • Author(s): H. Ichikawa,Y. Minamiyama
  • Organizer: International Conference on Food Factors 2011
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Calorie restriction improves diabetic complications via reduction of mitochondrial reactive oxygen species in Type II diabetic rats. (2011)
  • Author(s): Y. Minamiyama, S. Takemura,H. Ichikawa, S. Okada and T. Yoshikawa
  • Organizer: 4th INTERNATIONAL SYMPOSIUM NUTRITION, OXYGEN BIOLOGY, AND MEDICINE
  • Place of Presentation: Paris, France
• [Presentation] The role of histidine on wound healing using cultured rat intestinal epithelial cells. (2011)
  • Author(s): K. Hori, H. Ichikawa,T. Takagi, Y. Minamiyama, M. Nakamura, A. Doi, Y. Kawabata, T. Kaneda, K. Uchiyama, Y. Naito, T. Yoshikawa
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
• [Presentation] The histidine affects delayed wound healing induced by indomethacin using cultured rat epithelial cells. (2011)
  • Author(s): S. Matsuo, H. Ichikawa, Y. Minamiyama, T. Takagi, R. Fuchiue, A. Emoto, M. Suetsugu, K. Uchiyama, Y. Naito, T. Yoshikawa
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
• [Presentation] The effect of Kampo Medicine "Dai-kenchu-to" on wound healing of rat intestinal epithelial cells. (2011)
  • Author(s): H. Asai, H. Ichikawa, Y. Minamiyama, T. Takagi, A. Wada, Y. Ozaki, K. Okazaki, C. Minami, O. Handa, Y. Naito, T. Yoshikawa
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
• [Presentation] Sperm dysfunction from aging is involved in the oxidative stress. (2011)
  • Author(s): R. Yamamoto, Y. Minamiyama, H. Ichikawa, T. Takagi, A. Ishii, Y. Sato, E. Miyanishi, M. Yasui, O. Handa, Y. Naito, T. Yoshikawa
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
• [Presentation] Generation of reactive oxygen species from the sperm mitochondria of rats is an excellent marker for the biological toxicity of endocrine-disrupting chemicals. (2011)
  • Author(s): E. Miyanishi, Y. Minamiyama, IchikawaH, T. Takagi, A. Ishii, O. Handa, Y. Naito, T. Yoshikawa
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
• [Presentation] Protective effect of polaprezinc on acetylsalicylic acid-induced apoptosis of rat intestinal epithelial cells - involvement of heat shock protein 70 and reactive oxygen species. (2011)
  • Author(s): O. Handa, Y. Naito, A. Fukui, Y. Qin, Y. Uehara, T. Tsuji, H. Yoriki, R. Horie, K. Katada, K. Kamada, K. Uchiyama, T. Ishikawa, T. Takagi, Y. Minamiyama, H. Konishi, N. Yagi, S. Kokura, H. Ichikawa, T. Yoshikawa
  • Organizer: 5th SFRR-ASIA 8th ASMRM11th J-mit
  • Place of Presentation: Kagoshima, Japan
• [Presentation] Effects of aging on sperm function and oxidative stress. (2010)
  • Author(s): Y. Minamiyama, H. Ichikawa, TakemuraS, T. Kawagishi, A. Wakahara, C. Minami, M. Yasui, R. Yamamoto, NaitoY, T. Yoshikawa
  • Organizer: 15th Biennial Meeting of the Society for Free Radical Research (International SFRR 2010)
  • Place of Presentation: Orland, FL
• [Presentation] Generation of reactive oxygen species from the sperm mitochondria of rats is an excellent marker for the biological toxicity of endocrine-disrupting chemicals. (2010)
  • Author(s): Y. Minamiyama, H. Ichikawa, S. Takemura, Y. Naito, T. Yoshikawa
  • Organizer: 7thconference of Asian Society for Mitochondrial Research and Medicine (ASMRM) and 10thconference of Japanese Society for Mitochondrial Research and Medicine (J-mit)
  • Place of Presentation: Fukuoka, Japan
• [Presentation] Calorie restriction improves endothelial nitric oxide synthase overexpression and production of mitochondrial reactive oxygen species of cardiovasculatures in type II diabetic rats (2010)
  • Author(s): Yukiko Minamiyama, Shigekazu Takemura, Hiroshi Ichikawa, Shintaro Kodai, Hiroji Shinkawa, Yuji Naito, Shigeru Okada, Toshikazu Yoshikawa.
  • Organizer: 第6回国際NO学会
  • Place of Presentation: 国立京都国際会館
• [Presentation] Generation of reactive oxygen species from the sperm mitochondria of rats is an excellent marker for the biological toxicity of endocrine-disrupting chemicals (2010)
  • Author(s): Yukiko Minamiyama, Hiroshi, Ichikawa, Shigekazu Takemura, Yuji Naito, Toshikazu Yoshikawa.
  • Organizer: 第10回アジアミトコンドリア学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Effects of aging on sperm function and oxidative stress (2010)
  • Author(s): Yukiko Minamiyama, Hiroshi Ichikawa, Shigekazu Takemura, Takahiro Kawagishi, Ayako Wakahara, Chikako Minami, Madolca Yasui, Ryuhei Yamamoto, Yuji Naito, Toshikazu Yoshikawa
  • Organizer: 第17回国際フリーラジカル学会
  • Place of Presentation: Caribe Royale All Suites Hotel & Convention Center(Orland)
• [Presentation] ヒト好塩基球の高親和性IgE受容体刺激によるアレルギー反応におよぼす穀物発酵食品の効果 (2010)
  • Author(s): 南山幸子, 竹村茂一, 市川寛, 若原綾子, 安井まどか, 川岸崇裕, 内藤裕二, 吉川敏一
  • Organizer: 第10回日本抗加齢学会
  • Place of Presentation: 国立京都国際会館
• [Presentation] 加齢による精子機能と酸化ストレス (2010)
  • Author(s): 南山幸子, 川岸崇裕, 市川寛, 竹村茂一, 若原綾子, 南千花子, 安井まどか, 内藤裕二, 吉川敏一
  • Organizer: 第63回日本酸化ストレス学会
  • Place of Presentation: 神奈川県民ホール
• [Presentation] 2型糖尿病併存時の肝切除後肝再生不全と機能障害の解析 (2010)
  • Author(s): 南山幸子, 竹村茂一, 市川寛, 吉川敏一
  • Organizer: 第14回日本肝臓学会
  • Place of Presentation: パシフィコ横浜
• [Book] 酸化ストレスとAOB 第2版 (2011)
  • Author(s): 岡田茂,南山幸子
  • Total Pages: 105
  • Publisher: マイクロ出版
• [Book] Functional Food(イチョウ葉エキス,9:220-225) (2010)
  • Author(s): 南山幸子, 吉川敏一
  • Total Pages: 288
  • Publisher: フジメディカル出版
• [Remarks]
• [Patent(Industrial Property Rights)] 白血球活性化抑制剤 (2011)
  • Inventor(s): 竹村茂一, 新川寛二, 岡真優子, 東秀紀, 南山幸子
  • Industrial Property Rights Holder: 公立大学法人大阪市立大学, 京都府公立大学法人京都府立大学
  • Industrial Property Number: 2011-172116
  • Filing Date: 2011