Development of probiotics for preventing opportunistic infections of fish

• Project/Area Number: 22580211
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Nihon University
• Principal Investigator: SUGITA Haruo 日本大学, 生物資源科学部, 教授 (50139052)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: プロバイオティクス / 魚類 / 腸内細菌叢 / AHL / 日和見感染症 / 16S rRNA遺伝子 / 魚類腸内細菌 / N-アシルホモセリンラクトン / Aeromonas属細菌 / 細菌叢 / ブロバイオティクス

Research Abstract

The intestinal tracts of ayu (Plecoglossus altivelis), Japanese smelt (Hypomesus nipponensis) and goldfish (Carassius auratus) were colonized with the AHL-producing aeromonads. On the other hand, members of genus Shewanella were isolated as AHL-decomposer from the intestinal tract of goldfish. These results suggest that the AHL-decomposing Shewanella seems to be one of the candidate of probiont for preventing aneromonasis of freshwater fish.

Research Products

• [Journal Article] ヒイラギ腸管内の硫酸還元細菌について. (2012)
  • Author(s): 陳家輝、相良和之、糸井史朗、杉田治男
  • Journal Title: 水産増殖 Volume: 60巻 Pages: 519-521
  • NAID: 130005062485
  • Peer Reviewed
• [Journal Article] クローンライブラリー法による沿岸魚類腸内細菌群集の解析 (2012)
  • Author(s): 田中祐輔、陳家輝、小崎照邦、込宮雄介、糸井史朗、杉田治男
  • Journal Title: 水産増殖 Volume: 60巻 Pages: 333-340
  • NAID: 10031122364
  • Peer Reviewed
• [Journal Article] Effect of the C-terminal domain of Vibrio proteolyticus chitinase A on the chitinolytic activity in association with pH changes. (2012)
  • Author(s): S. Itoi, Y. Kanomata, S. Uchida, K. Kadokura, T. Nishio, T. Oku, H. Sugita
  • Journal Title: Letters inApplied Microbiology Volume: 54巻 Pages: 441-446
  • Peer Reviewed
• [Journal Article] Bacterial diversity of the upper and lower intestines of the common carp (Cyprinus carpio Linnaeus, 1758) reared in an indoor tank. (2012)
  • Author(s): H. Sugita, H. Mizuki
  • Journal Title: Journal of AppliedIchthyology Volume: 28巻 Issue: 4 Pages: 647-648
  • DOI: 10.1111/j.1439-0426.2012.01965.x
  • Peer Reviewed
• [Journal Article] 養殖ワカサギ腸内細菌のN-アシルホモセリンラクトン生産能. (2012)
  • Author(s): 陳家輝,小川真幸,相良和之,糸井史朗,杉田治男
  • Journal Title: 日水誌 Volume: 78巻 Pages: 259-261
  • NAID: 10030124086
  • Peer Reviewed
• [Journal Article] Diversity of siderophore-producing bacteria isolated from the intestinal tracts of fish along the Japanese coast. (2012)
  • Author(s): H. Sugita, H. Mizuki, S. Itoi
  • Journal Title: Aquaculture Research Volume: 43巻 Issue: 4 Pages: 481-488
  • DOI: 10.1111/j.1365-2109.2011.02851.x
  • Peer Reviewed
• [Journal Article] Effect of the C-terminal domain of Vibrio proteolyticus chitinase A on the chitinolytic activity in association with pH changes (2012)
  • Author(s): S. Itoi
  • Journal Title: Letters in Applied Microbiology Volume: 54 Issue: 5 Pages: 441-446
  • DOI: 10.1111/j.1472-765x.2012.03228.x
  • Peer Reviewed
• [Journal Article] クローンライブラリー法による沿岸魚類腸内細菌群集の解析 (2012)
  • Author(s): 田中祐輔
  • Journal Title: 水産増殖 Volume: 60 Pages: 333-340
  • Peer Reviewed
• [Journal Article] ヒイラギ腸管内の硫酸還元細菌について (2012)
  • Author(s): 陳 家輝
  • Journal Title: 水産増殖 Volume: 60 Pages: 519-521
  • Peer Reviewed
• [Journal Article] 養殖ワカサギ腸内細菌のN-アシルホモセリンラクトン生産能 (2012)
  • Author(s): 陳家輝, 小川真幸, 相良和之, 糸井史朗, 杉田治男
  • Journal Title: 日本水産学会誌 Volume: 78 Pages: 259-261
  • NAID: 10030124086
  • Peer Reviewed
• [Journal Article] 養殖アユ腸管から分離されたN-アシルホモセリンラクトン生産菌. (2011)
  • Author(s): 小川真幸、陳家輝、糸井史朗、杉田治男
  • Journal Title: 水産増殖 Volume: 59巻 Pages: 595-600
  • NAID: 10031122313
  • Peer Reviewed
• [Journal Article] 養殖アユ腸管から分離されたN-アシルホモセリンラクトン生産菌 (2011)
  • Author(s): 小川真幸、陳家輝、糸井史朗、杉田治男
  • Journal Title: 水産増殖 Volume: 59 Pages: 595-600
  • NAID: 10031122313
  • Peer Reviewed
• [Journal Article] Culturable bacterial flora in the intestinal tract of Japanese pufferfish Takifugu rubripes. (2010)
  • Author(s): H. Sugita, K. Sugiyama, S. Itoi
  • Journal Title: Aquaculture Science Volume: 58巻 Pages: 437-438
  • NAID: 130004888423
  • Peer Reviewed
• [Journal Article] Culturable bacterial flora in the intestinal tract of Japanese pufferfish Takifugu rubripes (2010)
  • Author(s): H.Sugita, K.Sugiyama, S.Itoi
  • Journal Title: Aquaculture Science Volume: 58 Pages: 437-438
  • NAID: 130004888423
  • Peer Reviewed
• [Presentation] 海洋性乳酸菌Lactococcus lactissubsp.lactisのトラフグ腸管への定着性に関する研究. (2013)
  • Author(s): 高梨志保里、宮之本昴也、糸井史朗、杉田治男.
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京)
  • Year and Date: 2013-03-29
• [Presentation] Vibrio科細菌検出プライマーの有効性. (2013)
  • Author(s): 相良和之、陳家輝、糸井史朗、杉田治男.
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京).
  • Year and Date: 2013-03-27
• [Presentation] Sulfate-reducing bacteria in the intestinal tract of spotnape ponyfish Leiognathus nuchalis. 10th Japan-Korea (2012)
  • Author(s): K. Sagara, C. H. Chen, S. Itoi, H. Sugita.
  • Organizer: Korea-Japan Joint Symposium on Aquaculture 2012
  • Place of Presentation: Nagasaki.
  • Year and Date: 2012-12-08
• [Presentation] Colonization of Lactococcus lactis subsp. lactis strains in the intestinal tract of goldfish. (2012)
  • Author(s): J. Uchida, A. Miura, S. Itoi, H.Sugita.
  • Organizer: The 9th Asia-Pacific Marine Biotechnology Conference
  • Place of Presentation: Kochi, Japan.
  • Year and Date: 2012-07-14
• [Presentation] Microbial communities in intestinal tracts of Japanese coastal fish. (2012)
  • Author(s): C.H. Chen, Y. Tanaka, K. Sagara, S. Itoi, H.Sugita.
  • Organizer: The 9th Asia-Pacific Marine Biotechnology Conference
  • Place of Presentation: Kochi, Japan.
  • Year and Date: 2012-07-13
• [Presentation] I腸内細菌研究の現状認識.3.魚類の腸内細菌叢.シンポジウム:水産「プロバイオティクス」の創成 (2012)
  • Author(s): 杉田治男.
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京).
  • Year and Date: 2012-03-30
• [Presentation] 水産「プロバイオティクス」の創成:I-3魚類の腸内細菌叢 (2012)
  • Author(s): 杉田治男
  • Organizer: 平成24年度日本水産学会春季大会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2012-03-30
• [Presentation] 乳酸菌Lactococcus lactis subsp.lactisの海水魚類腸管への定着性. (2012)
  • Author(s): 三浦愛、糸井史朗、杉田治男
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京)
  • Year and Date: 2012-03-28
• [Presentation] 沿岸魚類の腸内細菌群集について. (2012)
  • Author(s): 陳家輝、田中祐輔、相良和之、糸井史朗、杉田治男.
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京)
  • Year and Date: 2012-03-28
• [Presentation] 沿岸魚類の腸内細菌群集について (2012)
  • Author(s): 陳家輝、杉田治男, 他3名
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-28
• [Presentation] 乳酸菌Lactococcus lactis subsp.lactisの海水魚類腸管への定着性 (2012)
  • Author(s): 三浦愛、糸井史朗、杉田治男
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-28
• [Presentation] Possibility of marine Lactococcus lactis subsp.lactis strain as probiotic for the pufferfish Takifugu rubripes. (2011)
  • Author(s): A. Miura, S. Takanashi, S. Itoi, H. Sugita.
  • Organizer: The International Symposium on Muscle Biochemistry, University of Tokyo
  • Place of Presentation: Tokyo
  • Year and Date: 2011-10-28
• [Presentation] Advantage ofchitin oligosaccharides utilization in Lactococcus lactis strain from marine environment. (2011)
  • Author(s): S. Takanashi, S. Itoi, H. Sugita.
  • Organizer: The International Symposium on Muscle Biochemistry University of Tokyo
  • Place of Presentation: Tokyo
  • Year and Date: 2011-10-28
• [Presentation] 由来の異なるLactococcus lactis subsp.lactisの宿主選択性. (2011)
  • Author(s): 三浦愛、糸井史朗、杉田治男.
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎大学(長崎).
  • Year and Date: 2011-10-01
• [Presentation] 由来の異なるLactococcus lactis subsp.lactisの宿主選択性 (2011)
  • Author(s): 三浦愛、糸井史朗、杉田治男
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎
  • Year and Date: 2011-10-01
• [Presentation] Sulphate-reducing bacteria in the eelgrass bed of the Izu Peninsula (2011)
  • Author(s): C.H. Chen, Y. Tanaka, T. Kozaki, S. Itoi, K.Yoshihara, H. Sugita.
  • Organizer: Japan. IUMS2011
  • Place of Presentation: Sapporo.
  • Year and Date: 2011-09-07
• [Presentation] Microflora of the intestinal tracts of fish along the Japanese coast. (2011)
  • Author(s): Y. Tanaka, C.H. Chen, T. Kozaki, Y. Komiya,S. Itoi, H. Sugita.
  • Organizer: IUMS2011
  • Place of Presentation: Sapporo.
  • Year and Date: 2011-09-07
• [Presentation] Microflora of the intestinal tracts of fish along the Japanese coast (2011)
  • Author(s): Y.Tanaka, H.Sugita, 他4名
  • Organizer: IUMS2011
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-07
• [Presentation] Sulphate-reducing bacteria in the eelgrass bed of the Izu Peninsula, Japan (2011)
  • Author(s): C.H.Chen, H.Sugita, 他4名
  • Organizer: IUMS2011
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-07
• [Presentation] Artemia cysts and nauplii hatched under laboratory conditions (2011)
  • Author(s): H.Kurihara, H.Sugita, 他4名
  • Organizer: IUMS2011
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-07
• [Presentation] クローンライブラリィ法による沿岸魚類腸内細菌叢の解析. (2011)
  • Author(s): 田中祐輔、陳家輝、小崎輝邦、糸井史朗、杉田治男.
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京)
  • Year and Date: 2011-03-30
• [Presentation] Microbial communities in the intestinal tracts of coastal fish. (2010)
  • Author(s): Y. Tanaka, C.H. Chen, T. Kozaki, S. Itoi, H.Sugita.
  • Organizer: 2010Korea-Japan, Japan-Korea Joint Symposium on Aquaculture
  • Place of Presentation: Busan, Korea.
  • Year and Date: 2010-11-13
• [Presentation] The AHL-producing bacteria in the intestinal tracts of ayu Plecoglossus altivelis. (2010)
  • Author(s): M. Ogawa, S. Itoi, H. Sugita.
  • Organizer: 2010Korea-Japan, Japan-Korea Joint Symposium on Aquaculture
  • Place of Presentation: Busan, Korea.
  • Year and Date: 2010-11-13
• [Presentation] Microbial communities in the eelgrass bed of the Izu Peninsula (2010)
  • Author(s): C.H. Chen, T. Kozaki, Y. Tanaka, S. Itoi, K.Yoshihara, H. Sugita.
  • Organizer: Japan.2010 Korea-Japan, Japan-Korea Joint Symposium on Aquaculture
  • Place of Presentation: Busan, Korea.
  • Year and Date: 2010-11-13
• [Presentation] The AHL-producing bacteria in the intestinal tracts of ayu Plecoglossus altivelis (2010)
  • Author(s): M.Ogawa, S.Itoi, H.Sugita
  • Organizer: 2010 Korea-Japan, Japan-Korea Joint Symposium on Aquaculture
  • Place of Presentation: BEXCO, Busan, Korea
  • Year and Date: 2010-11-13
• [Presentation] Microflora of Artemia nauplii hatched under experimental conditions (2010)
  • Author(s): H.Kurihara, H.Sugita, 他4名
  • Organizer: 2010 Korea-Japan, Japan-Korea Joint Symposium on Aquaculture
  • Place of Presentation: BEXCO, Busan, Korea
  • Year and Date: 2010-11-13
• [Presentation] Microbial communities in the intestinal tracts of coastal fish (2010)
  • Author(s): Y.Tanaka, H.Sugita, 他3名
  • Organizer: 2010 Korea-Japan, Japan-Korea Joint Symposium on Aquaculture
  • Place of Presentation: BEXCO, Busan, Korea
  • Year and Date: 2010-11-13
• [Presentation] Characterization of amarine lactic acid bacterium Lactococcus lactis subsp. lactis isolated from the pufferfish. (2010)
  • Author(s): S. Itoi, H. Sugita.
  • Organizer: 9^<th>International Marine BiotechnologyConference
  • Place of Presentation: Quigdao, China.
  • Year and Date: 2010-10-10
• [Presentation] アルテミア耐久卵およびノープリウスの細菌叢 (2010)
  • Author(s): 栗原はるか、杉田治男, 他4名
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都大学、京都市
  • Year and Date: 2010-09-24
• [Presentation] Microbial communities in intestinal tracts of Japanese coastal fish
  • Author(s): Chia-Hui Chen
  • Organizer: The 9th Asia-Pacific Marine Biotechnology Conference
  • Place of Presentation: 高知
• [Presentation] Characterization of lactic acid bacteria isolated from intestinal contents of the bivalve Meretrix lamarckii
  • Author(s): Shihori Takanashi
  • Organizer: The 9th Asia-Pacific Marine Biotechnology Conference
  • Place of Presentation: 高知
• [Presentation] Colonization of Lactococcus lactis subsp. lactis strains in the intestinal tract of goldfish
  • Author(s): Junya Uchida
  • Organizer: The 9th Asia-Pacific Marine Biotechnology Conference
  • Place of Presentation: 高知
• [Presentation] Sulfate-reducing bacteria in the intestinal tract of spotnape ponyfish Leiognathus nuchalis
  • Author(s): Kazuyuki Sagara
  • Organizer: 10th Japan-Korea, Korea-Japan Joint Symposium on Aquaculture 2012
  • Place of Presentation: 長崎
• [Presentation] Effect of oxytetracycline on the microbial community in the bottom sand of the pufferfish-rearing aquarium
  • Author(s): Ryo Nagamitsu
  • Organizer: 10th Japan-Korea, Korea-Japan Joint Symposium on Aquaculture 2012
  • Place of Presentation: 長崎
• [Presentation] Isolation and characterization of lactic acid bacteria from intestinal contents of hard clam, Meretrix lamarckii
  • Author(s): Junya Uchida
  • Organizer: 10th Japan-Korea, Korea-Japan Joint Symposium on Aquaculture 2012
  • Place of Presentation: 長崎
• [Presentation] ハマグリ腸内容物由来乳酸菌の分離および特性
  • Author(s): 内田潤也
  • Organizer: 平成２５年度日本水産学会春季大会
  • Place of Presentation: 東京
• [Presentation] Vibrio科細菌検出プライマーの有効性
  • Author(s): 相良和之
  • Organizer: 平成２５年度日本水産学会春季大会
  • Place of Presentation: 東京
• [Book] 海の環境微生物学 (2011)
  • Author(s): 石田祐三郎・杉田治男
  • Total Pages: 249
  • Publisher: 恒星社厚生閣
• [Book] 食品微生物学辞典 (2010)
  • Author(s): 杉田治男
  • Total Pages: 341
• [Book] 増補改訂版・海の環境微生物学 (2010)
  • Author(s): 石田祐三郎・杉田治男(編著)
  • Total Pages: 249
  • Publisher: 恒星社厚生閣