Bactericidal function of neutrophils in Crohn`s disease

• Project/Area Number: 22590708
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Keio University
• Principal Investigator: MATSUOKA Katsuyoshi 慶應義塾大学, 医学部, 助教 (40307393)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: クローン病 / 好中球 / 殺菌能 / CARD15 / IL-6

Research Abstract

The etiology of Crohn’s disease (CD), characterized by chronic intestinal inflammation, has not yet been identified. Interestingly, chronic granulomatous disease, in which neutrophil bactericidal function is impaired due the congenital defect of NADPH oxi
1. Cytokine production
2. Phagocytosis
3.Reactive oxygen species (ROS) production
4. Apoptosis, and
6. Anti-microbial peptide (AMP) production. While no difference was observed in terms of apoptosis, phagocytosis, ROS production, or AMP production, neutrophils of CD patients produced significantly less IL-6 and IL-1 E compared with healthy controls. Fur

Research Products

• [Journal Article] Mucosal CXCR4+ IgG plasma cells contribute to the pathogenesis of human ulcerative colitis through FcγR-mediated CD14 macrophage activation. (2012)
  • Author(s): Uo M, Hisamatsu T, Miyoshi J, Kaito D,Yoneno K, Kitazume MT, Mori M, Sugita A,Koganei K, Matsuoka K, Kanai T, Hibi T.
  • Journal Title: Gut Volume: (in press)
  • Peer Reviewed
• [Journal Article] T-helper17 and interleukin-17-producing lymphoid tissue inducer-like cells make different contributions to colitis in mice. (2012)
  • Author(s): Ono Y, Kanai T, Sujino T, Nemoto Y,Kanai Y, Mikami Y, Hayashi A, MatsumotoA, Takaishi H, Ogata H, Matsuoka K,Hisamatsu T, Watanabe M,Hibi T.
  • Journal Title: Gastroenterology. Volume: 143(5) Pages: 1288-97
  • Peer Reviewed
• [Journal Article] CCR9(+) macrophages are required for eradication of peritoneal bacterial infections and prevention of polymicrobial sepsis. (2012)
  • Author(s): Mizukami T, Kanai T, Mikami Y, HayashiA, Doi T, Handa T, Matsumoto A, Jun L,Matsuoka K, Sato T, Hisamatsu T, Hibi T.
  • Journal Title: Immunol Lett. Volume: 147 Pages: 75-9
  • Peer Reviewed
• [Journal Article] Bile acids induce monocyte differentiation toward interleukin-12 hypo-producing dendritic cells via a TGR5-dependent pathway. (2012)
  • Author(s): Ichikawa R, Takayama T, Yoneno K,Kamada N, Kitazume MT, Higuchi H,Matsuoka K, Watanabe M, Itoh H, Kanai T,Hisamatsu T, Hibi T.
  • Journal Title: Immunology. Volume: 136 Pages: 153-62
  • Peer Reviewed
• [Journal Article] Ectopic expression of blood type antigens in inflamed mucosa with higher incidence of FUT2 secretor status in colonic Crohn's disease. (2011)
  • Author(s): Miyoshi J, Yajima T, OkamotoS, Matsuoka K, Inoue N, Hisamatsu T,Shimamura K, Nakazawa A, Kanai T, OgataH, Iwao Y, Mukai M, Hibi T.
  • Journal Title: J Gastroenterol. Volume: 46 Pages: 1056-1063
  • NAID: 10029706720
  • Peer Reviewed
• [Journal Article] Regulatory T Cells Suppress the Development of Colitis, Blocking Differentiation of T-helper 17 Into Alternative T-Helper 1 Cells. (2011)
  • Author(s): Sujino T, Kanai T, Ono Y, Mikami Y,Hayashi A, Doi T, Matsuoka K, HisamatsuT, Takaishi H, Ogata H, Yoshimura A,Littman DR, Hibi T.
  • Journal Title: Gastroenterology Volume: 141 Pages: 1014-1023
  • Peer Reviewed
• [Journal Article] Intracellular bacteria recognition contributes to maximal interleukin (IL)-12 production by IL-10-deficient macrophages. (2011)
  • Author(s): Naruse H, Hisamatsu T, Yamauchi Y,Chang JE, Matsuoka K, Kitazume MT, AraiK, Ando S, Kanai T, Kamada N, Hibi T.
  • Journal Title: Clin Exp Immunol. Volume: 164 Pages: 137-144
  • Peer Reviewed
• [Journal Article] Competition between colitogenic Th1 and Th17 cells contributes to the amelioration of colitis. (2010)
  • Author(s): Mikami Y, Kanai T, Sujino T, Ono Y,Hayashi A, Okazawa A, Kamada N,Matsuoka K, Hisamatsu T, Okamoto S,Takaishi H, Inoue N, Ogata H, Hibi T.
  • Journal Title: Eur J Immunol. Volume: 40 Pages: 2409-2422
  • Peer Reviewed