Enhanced safety of infertile therapy by means of quality control of sperm - relationship between vacuole in sperm head and DNA fragmentation

Research Project

Project/Area Number	22591837
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Obstetrics and gynecology
Research Institution	Tokyo Dental College
Principal Investigator	KANEKO Satoru 東京歯科大学, 歯学部, 講師 (40214457)
Project Period (FY)	2010 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords	品質管理 / 精子頭部空胞 / DNA 損傷 / 顕微授精 / 染色体 / DNA損傷 / ヒト精子 / DNA / 電気泳動 / DNA断片化 / DNA fiber
Research Abstract	The early stage of DNA fragmentation in a single nucleus could be observed by single cell pulse field gel electrophoresis (SCPFGE). First, a few large fibrous fragments were derived from a bundle of long chain fibers, the cleavages were advanced until finally almost all the DNA was shredded to granular fragments. There found no significant correlation between DNA fragmentation and vacuole in sperm head. The cell culture under high oxygen concentration caused increment of the percentage of DNA fragmentation.