| Project/Area Number |
22651076
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
System genome science
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| Research Institution | Osaka University |
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Principal Investigator |
KODAMA Tetsuya 大阪大学, 大学院・薬学研究科, 助教 (00432443)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,530,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | メチル化 / オリゴヌクレオチド / 複合体化 / 人工核酸 / 三重鎖形成核酸 / アルキル化 |
|---|
| Research Abstract |
Methylation of the cytosine base at C5 position was important modification concerning gene expression, cellular differentiation, virus infection and so on. Toward the development of the triplex-forming-oligonucleotide-based chemical technique that induces the methylation, a synthetic method of oligonucleotide conjugating a methylsulfonium group was established. As a result of the treatment of target DNA with a methyl sulfonium conjugating oligonucleotide, some DNA fragments derived from the methylation(alkylation) on the guanine bases were formed. It is important that triplex forming oligonucleotide conjugating an alkylating agent induced DNA alkylation, which is the first step toward the methylation of cytosine.
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