Creation of artificial clock proteins to induce rhythmic gene expression
Project/Area Number |
22710213
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Living organism molecular science
|
Research Institution | Kyoto University |
Principal Investigator |
IMANISHI Miki 京都大学, 化学研究所, 助教 (80362391)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 遺伝子発現 / ジンクフィンガー / 人工転写因子 / タンパク質デザイン / 概日時計 |
Research Abstract |
An artificial zinc finger transcription factor targeting a core clock gene was designed and it successfully changed the circadian time. A simple system to manipulate gene expression patterns to be circadian was also constructed using a zinc finger motif. These artificial proteins would be useful for elucidating the mechanism of rhythmic gene expression and for manipulating gene expression.
|
Report
(3 results)
Research Products
(23 results)