Microtubule regulation by polyglycylation

• Project/Area Number: 22770191
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Kitasato University (2012-2013); Hamamatsu University School of Medicine (2010-2011)
• Principal Investigator: TSUTSUMI KOJI 北里大学, 理学部, 助教 (50569853)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 微小管 / 翻訳後修飾 / アクチン細胞骨格 / 細胞極性 / シグナル伝達 / 細胞骨格 / アクチン繊維 / 細胞運動 / ポリグリシン化

Research Abstract

Cytoskeleton is crucial for cell morphology and cell polarity. I have studied (1) the role of polyglycylation of tubulin and (2) a novel regulatory mechanism of FilGAP, key regulator of actin cytoskeleton remodeling. I found decreased polyglycylation and higher glutamylation level of tubulin in TTLL8 knockout mice testes and spermatozoa. Antagonistic regulation between glycylation and glutamylation of tubulins may generate tubulin diversity. (2) I found that Clathrin regulates actin cytoskeleton remodeling through the direct inhibition of FilGAP.

Research Products

• [Presentation] クラスリンはFilGAPを阻害しアクチン細胞骨格リモデリングを制御する (2014)
  • Author(s): Koji Tsutsumi, Yoshio Shibagaki, Seisuke Hattori, and Yasutaka Ohta
  • Organizer: 第6回日本細胞生物学会大会
  • Place of Presentation: 奈良県新公会堂(奈良県奈良市)
• [Presentation] Clathrin regulates actin remodeling through the direct inhibition of FilGAP (2013)
  • Author(s): 堤 弘次
  • Organizer: 第66回日本細胞生物学会
  • Place of Presentation: 奈良
• [Presentation] Role of mono-glycine ligase TTLL8 in male reproductive system (2012)
  • Author(s): 堤 弘次
  • Organizer: 第64回日本細胞生物学会
  • Place of Presentation: 神戸
• [Presentation] Role of mono-glycine ligase TTLL8 in male reproductive system (2011)
  • Author(s): 堤 弘次
  • Organizer: 第63回日本細胞生物学会
  • Place of Presentation: 札幌