Detection of a defense system of intestinal mucosa as a novel examination tool for canine inflammatory bowel disease

• Project/Area Number: 22780279
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Clinical veterinary science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: IDE Kaori 東京農工大学, 大学院・農学研究院, 助教 (40550281)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 犬 / 炎症性腸疾患 / 腸管型アルカリフォスファターゼ / 腸管粘膜上皮 / 獣医内科学 / 腸炎 / IBD / 酵素

Research Abstract

Intestinal alkaline phosphatase (iALP) was found to be expressed with enzymatic activity, more intensively in mucosal epithelia of duodenum than of colon, in dogs. Dephosphorylation reaction of lipopolysaccharide (LPS), which is the core function of the defense mechanism by iALP against excessive intestinal microflora, was also proven to be active. Fecal iALP concentration was shown to be high in cases of severe inflammatory bowel disease with high clinical activity score.

Research Products

• [Journal Article] Comparison of the expression, activity, and fecal concentration of intestinal alkaline phosphatase between healthy dogs and dogs with chronic enteropathy (2016)
  • Author(s): Kaori Ide, Kazuki Kato, Yuki Sawa, Akiko Hayashi, Rei Takizawa, Koji Nishifuji
  • Journal Title: American Journal of Veterinary Research Volume: 7 Issue: 7 Pages: 721-729
  • DOI: 10.2460/ajvr.77.7.721
  • Peer Reviewed
• [Journal Article] Expression Analysis of Desmosomal Components of the Novel Canine Epidermal Keratinocyte Cell Line (MSCEK). (2010)
  • Author(s): Ide K, Yamamoto W, Sato M, Yabuzoe A, Iwasaki T, Nishifuji K.
  • Journal Title: Journal of Veterinary Medical Science Volume: 72 Pages: 1479-1482
  • NAID: 130000264190
  • Peer Reviewed
• [Journal Article] Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs (2010)
  • Author(s): Iyori, K., Hisatsune, J., Kawakami, T., Shibata, S., Murayama, N., Ide, K., Nagata, M., Fukata, T., Iwasaki, T., Oshima, K., Hattori, M., Sugai, M., Nishifuji, K.
  • Journal Title: FEMS Microbiology Letters Volume: 312 Pages: 169-175
  • Peer Reviewed
• [Presentation] 犬の腸管型アルカリフォスファターゼに関する検討 (2012)
  • Author(s): 井手香織、岩崎望、松本英、平塚彰悟、石下進平、西藤公司、岩崎利郎
  • Organizer: 日本獣医内科学アカデミー学術大会
  • Place of Presentation: 横浜
  • Year and Date: 2012-02-18
• [Presentation] 犬の腸管型アルカリフォスファターゼに関する検討 (2012)
  • Author(s): 井手香織
  • Organizer: 日本獣医内科学アカテミー/日本獣医臨床病理学会2012年大会
  • Place of Presentation: 横浜
  • Year and Date: 2012-02-18
• [Presentation] Determination of ear pinnae dermal blood flow using laser Doppler flow meter in dogs with auricular ischemic dermatoses and healthy dogs (2010)
  • Author(s): K.Ide, M.Tokunaga, H.Ninomiya, T.Iwasaki, K.Nishifuji
  • Organizer: 第24回ヨーロッパ獣医皮膚科学会年次大会
  • Place of Presentation: フィレンツェ(イタリア)
  • Year and Date: 2010-09-14
• [Remarks]
  • URL: http://www.tuat-amc.org/laboratory/
• [Remarks]
  • URL: http://www.tuat-amc.org/laboratory/