Development of target-specific gene therapy system by using cell surface adhesive interferon.

• Project/Area Number: 22790034
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Physical pharmacy
• Research Institution: Kyoto University
• Principal Investigator: TAKAHASHI Yuki 京都大学, 大学院・薬学研究科, 助教 (00547870)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ドラッグデリバリー / 遺伝子治療 / インターフェロン / 薬学

Research Abstract

In the present study, we developed IFN-γderivative that can adhere on cellular surface in order to selectively increase IFN-γconcentration at gene delivery site. Developed IFN-γwas demonstrated to be able to adhere on the cell surface and selective biological activity obtained by gene delivery of the developed IFN-γderivative was specific in the gene-delivered organ. We found that the developed system was effective in reducing adverse side effect without reducing antitumor effect at target tissue compared to the normal IFN-γgene therapy system.

Research Products

• [Presentation] 機能性ペプチド融合によるIFN-γ時空間制御 (2012)
  • Author(s): 安藤満、高橋有己、西川元也、高倉喜信
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2012-03-31
• [Presentation] 細胞表面接着型インターフェロンを利用した標的特異的作用型遺伝子治療システムの開発 (2011)
  • Author(s): 高橋有己、安藤満、西川元也、渡部好彦、高倉喜信
  • Organizer: 第61回日本薬学会近畿支部総会
  • Place of Presentation: 神戸学院大学(兵庫県)
  • Year and Date: 2011-10-22
• [Presentation] Design and hydrodynamic gene transfer of 'sticky' IFNγfor liver-directed gene therapy. (2011)
  • Author(s): Mitsuru Ando, Yuki Takahashi, Hanae Mukumoto, Makiya Nishikawa, Yoshihiko Watanabe and Yoshinobu Takakura.
  • Organizer: American Society of Gene & Cell Therapy 14th Annual Meeting
  • Place of Presentation: Washington State Convention Center(Seatle, USA)
  • Year and Date: 2011-05-19
• [Presentation] Design and Hydrodynamic Gene Transfer of 'Sticky' interferon-γ for Liver-Directed Gene Therapy of Hepatic Metastasis of Cancer Cells (2011)
  • Author(s): Ando M, Takahashi Y, et al
  • Organizer: American society of Gene & Cell Therapy Annual Meeting 2013
  • Place of Presentation: シアトル(USA)
• [Presentation] 機能性ペプチド融合によるIFN-γ時空間制御 (2011)
  • Author(s): 安藤満、高橋有己、西川元也、高倉喜信
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(北海道)(招待講演)
• [Presentation] Design and gene delivery of 'sticky' interferonγto increase the effect/side-effect ratio for liver-directed interferon-γgene therapy (2010)
  • Author(s): Mitsuru Ando, Yuki Takahashi, Hanae Mukumoto, Makiya Nishikawa, Yoshihiko Watanabe, Yoshinobu Takakura
  • Place of Presentation: Ernest N. Morial Convention Center(New Orleans, USA)
  • Year and Date: 2010-11-17
• [Presentation] 体内動態制御によるインターフェロン遺伝子治療の最適化 (2010)
  • Author(s): 高橋有己、西川元也、渡部好彦、高倉喜信
  • Organizer: 第26回日本DDS学会
  • Place of Presentation: 大阪国際交流センター(大阪府)
  • Year and Date: 2010-06-17
• [Presentation] 体内動態制御によるインターフェロン遺伝子治療の最適化 (2010)
  • Author(s): 高橋有己、西川元也、高倉喜信
  • Organizer: 第26回 日本DDS学会
  • Place of Presentation: 大阪国際交流センター(大阪市)(招待講演)
• [Presentation] Design and gene delivery of 'sticky' interferon-γ to increase the effect/side-effect ratio for liver-directed interferon-γ gene therapy (2010)
  • Author(s): Ando M., et al.
  • Organizer: 2010 Pharmaceutical Sciences World Congress
  • Place of Presentation: Morial convention center (New Orleans, USA)