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The effect of individual differences in skeletal muscle protein degradation induced by pre-slaughter fasting on the meat quality of broiler chickens

Research Project

Project/Area Number 22K20616
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0605:Veterinary medical science, animal science, and related fields
Research InstitutionOkayama University

Principal Investigator

Katsumata Sachi  岡山大学, 環境生命自然科学学域, 特任助教 (60963824)

Project Period (FY) 2022-08-31 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsNτ-メチルヒスチジン / 絶食 / 骨格筋タンパク質分解 / 鶏肉品質 / ブロイラー / 遊離アミノ酸 / Nτ-メチルヒスチジン濃度 / 絶食ストレス / 骨格筋タンパク質分解反応
Outline of Research at the Start

と鳥前の絶食処理は、ブロイラーの骨格筋タンパク質分解反応を引き起こし鶏肉の熟成に影響することにより、鶏肉品質をばらつかせる可能性が高い。
本研究は「と鳥前の絶食ストレスに起因するブロイラーの骨格筋タンパク質分解反応の個体差が鶏肉品質に及ぼす影響の解明」を目的とする。
骨格筋タンパク質分解反応の指標として筋原線維タンパク質特異的な異化産物である血中Nτ-メチルヒスチジン濃度に着目し、Nτ-メチルヒスチジン濃度の個体差が鶏肉品質および鶏肉品質に寄与する骨格筋タンパク質分解経路に及ぼす影響を解明する。

Outline of Final Research Achievements

In this study, we conducted a pre-experiment with four fasting time levels in broiler chickens. There was a correlation between “skeletal muscle proteolysis level” and “free glutamic acid content in chicken meat after 48 hours of aging”. The second experiment was conducted with aligned fasting times. The results showed that there were individual differences in skeletal muscle proteolysis levels resulting from pre-slaughter fasting. Even under the same fasting time conditions, there was also a correlation between “skeletal muscle proteolysis level” and “free glutamic acid content in the aged chicken meat”, indicating that individual differences in skeletal muscle proteolysis induced by pre-slaughter fasting affect the meat quality.

Academic Significance and Societal Importance of the Research Achievements

本研究は、絶食ストレスに起因するタンパク質分解反応の個体差の指標として、骨格筋の筋原線維タンパク質特異的な異化産物(Nτ-メチルヒスチジン)に着目し実験を行った。これまで、骨格筋の異化産物をストレス指標として用いた研究はないが、骨格筋の異化産物は絶食ストレスに対する感受性の違いを検出できる。Nτ-メチルヒスチジンの濃度変化は、暑熱などの他のストレスを感受した際にも顕れるため、他のストレッサーに対する新たな非侵襲的評価法として応用が可能となり、動物福祉に貢献できると考えられる。

Report

(3 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • Research Products

    (4 results)

All 2024 2023 2022

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Effects of pre-slaughter fasting on antemortem skeletal muscle protein degradation levels and postmortem muscle free amino acid concentrations in broiler chickens2024

    • Author(s)
      Katsumata Sachi、Kamegawa Mizuki、Katafuchi Ayumi、Ohtsuka Akira、Ijiri Daichi
    • Journal Title

      Poultry Science

      Volume: 103 Issue: 2 Pages: 103307-103307

    • DOI

      10.1016/j.psj.2023.103307

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Pre-slaughter fasting changes the antemortem muscle proteolysis levels and postmortem meat quality2023

    • Author(s)
      S. Katsumata, M. kamegawa, A. Katafuchi, A. Ohtsuka, D. Ijiri
    • Organizer
      74th Annual Meeting of European Federation of Animal Science
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] と鳥前の絶食時間が肉用鶏における骨格筋タンパク質分解反応と熟成後の鶏肉品質との関係に及ぼす影響2023

    • Author(s)
      勝俣沙智, 亀川みずき, 片渕歩美, 大塚彰, 井尻大地
    • Organizer
      日本家禽学会 2023年度秋季大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Effects of feed withdrawal times on antemortem muscle protein degradation levels and postmortem muscle free amino acid concentrations in broiler chickens2022

    • Author(s)
      S. Katsumata, M. kamegawa, A. Katafuchi, A. Ohtsuka, D. Ijiri
    • Organizer
      68th International Congress of Meat Science and Technology
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research

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Published: 2022-09-01   Modified: 2025-01-30  

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