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Approaching the evolutionary origin of the sporophyte generation from multicellular developmental variation in a green alga

Research Project

Project/Area Number 22K20644
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0702:Biology at cellular to organismal levels, and related fields
Research InstitutionHokkaido University

Principal Investigator

Horinouchi Yusuke  北海道大学, 北方生物圏フィールド科学センター, 特任助教 (10962714)

Project Period (FY) 2022-08-31 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords生活環 / アオサ藻綱 / 世代交代 / 胞子体 / 進化 / 表現型多型 / 緑藻 / 発生多型 / 進化的起源
Outline of Research at the Start

多くの植物は胞子体と呼ばれる複相(2N)の接合子から発生する多細胞の体を持つ。植物の祖先は、単相(1N)の胞子が発生した多細胞の体である配偶体が主要な体だと考えられている。近年、胞子体の進化的起源として、配偶体の多細胞発生を司る遺伝子系が複相世代に流用され胞子体が獲得されたという仮説が着目されている。本課題では、祖先的な緑藻エゾヒトエグサで発見した接合子の発生多型を詳しく調べ、遺伝子系流用により胞子体が獲得された証拠の獲得を目指す。

Outline of Final Research Achievements

In many plants, the diploid multicellular body is called sporophytes. Sporophytes may have evolved from an ancestral alga with a unicellular diploid and multicellular haploid phase but its mechanism is largely unknown. In this project, I performed cultural experiments, ploidy analyses, and morphological comparisons to investigate a unique intraspecific variation found in an ancestral-like alga Monostroma angicava, where zygotes develop into both unicellular diploids and sporophytes. The results suggest that sporophytes are emerged by co-opting the gametophyte developmental program to the diploid phase. This study suggests the importance of developmental variation in early evolution of novel multicellular sporophytes.

Academic Significance and Societal Importance of the Research Achievements

植物は胞子体世代の獲得により多様化し、複雑性を進化させてきた。胞子体が配偶体の発生プログラムを流用して獲得されたという仮説は近年着目されているが、それが実際に生じるのか、どのように生じるのかをについて説得力のある証拠は乏しい。本研究成果は胞子体が発生多型として、配偶体の発生プログラムを大規模に流用して獲得された可能性を示唆し、祖先藻類で複相の単細胞から多細胞体制への遷移が生じた有り得うべき機構について知見をもたらす。

Report

(3 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • Research Products

    (11 results)

All 2024 2023 2022

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] Mitochondrial uniparental inheritance achieved after fertilization challenges the nuclear-cytoplasmic conflict hypothesis for anisogamy evolution2023

    • Author(s)
      Togashi Tatsuya、Parker Geoff A.、Horinouchi Yusuke
    • Journal Title

      Biology Letters

      Volume: 19 Issue: 9 Pages: 1-5

    • DOI

      10.1098/rsbl.2023.0352

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Unicellular and multicellular developmental variations in algal zygotes produce sporophytes2023

    • Author(s)
      Horinouchi Yusuke、Togashi Tatsuya
    • Journal Title

      Biology Letters

      Volume: 19 Issue: 10 Pages: 1-7

    • DOI

      10.1098/rsbl.2023.0313

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Toward an understanding of life cycle diversification in green seaweeds2023

    • Author(s)
      堀之内 祐介
    • Journal Title

      JAPANESE JOURNAL OF ECOLOGY

      Volume: 73 Issue: 2 Pages: 49-56

    • DOI

      10.18960/seitai.73.2_49

    • ISSN
      0021-5007, 2424-127X
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Presentation] 海産緑藻の生活環多様化の理解に向けた理論的枠組み2024

    • Author(s)
      堀之内祐介、大槻久、富樫辰也
    • Organizer
      第71回日本生態学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 海産緑藻エゾヒトエグサの微視的胞子体の越夏生育地2024

    • Author(s)
      堀之内祐介、望月康生、市原健介、富樫辰也
    • Organizer
      日本藻類学会第48回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Intraspecific variation can provide a framework to understand life cycle diversification in green algae2023

    • Author(s)
      Yusuke Horinouchi, Hisashi Ohtsuki, Tatsuya Togashi
    • Organizer
      2023 Ecological Society of America Annual Meeting
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Unicellular and multicellular developmental variations of zygotes cause a life cycle variation in Monostroma angicava (Ulotrichales, Ulvophyceae)2023

    • Author(s)
      Yusuke Horinouchi
    • Organizer
      37th Korean society of phycology
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 海藻と繊毛虫の見過ごされた相互作用2023

    • Author(s)
      堀之内 祐介、富樫 辰也
    • Organizer
      第70回日本生態学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] コディオルム体を持つ海産緑藻エゾヒトエグサの生活環とその種内多型2023

    • Author(s)
      堀之内 祐介
    • Organizer
      日本藻類学会第47回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Stochastic sporophyte development and implications for life cycle evolution in marine green algae2022

    • Author(s)
      Yusuke Horinouchi, Hisashi Ohtsuki, Tatsuya Togashi
    • Organizer
      INTECOL 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] 緑藻の発生多様性からみる生活環の進化2022

    • Author(s)
      堀之内 祐介
    • Organizer
      令和4 (2022) 年度北海道植物学会
    • Related Report
      2022 Research-status Report

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Published: 2022-09-01   Modified: 2025-01-30  

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