| Project/Area Number |
23220014
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Foundation for Advancement of International Science (2014)
Tokyo Institute of Technology (2011-2013) |
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Principal Investigator |
AKAIKE Toshihiro 公益財団法人国際科学振興財団, 再生医工学バイオマテリアル研究所, 所長 (30101207)
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| Co-Investigator(Kenkyū-buntansha) |
KOBATAKE Eiry 東京工業大学, 大学院総合理工学研究科, 教授 (00225484)
NAGAOKA Masato 福井大学, テニュアトラック推進本部, 助教 (90397050)
ISE Hirohiko 九州大学, 先導物質化学研究所, 准教授 (10324253)
HARADA Ichiro 東京工業大学, 生命理工学研究科, 講師 (00361759)
賀喜 白乙 東京工業大学, 大学院・生命理工学研究科, 特任助教 (20515168)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥204,490,000 (Direct Cost: ¥157,300,000、Indirect Cost: ¥47,190,000)
Fiscal Year 2014: ¥44,720,000 (Direct Cost: ¥34,400,000、Indirect Cost: ¥10,320,000)
Fiscal Year 2013: ¥44,720,000 (Direct Cost: ¥34,400,000、Indirect Cost: ¥10,320,000)
Fiscal Year 2012: ¥54,860,000 (Direct Cost: ¥42,200,000、Indirect Cost: ¥12,660,000)
Fiscal Year 2011: ¥60,190,000 (Direct Cost: ¥46,300,000、Indirect Cost: ¥13,890,000)
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| Keywords | ES/iPS細胞 / 再生医工学 / 細胞用まな板 / 細胞マトリックス設計 / 神経細胞 / E-カドヘリン / N-カドヘリン / 均一系肝細胞反応 / Ｅ－カドヘリン / Ｎ－カドヘリン / ES/iPS / 再生医療 / パターニング培養 / 神経分化 / 肝細胞 / 心筋細胞 |
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| Outline of Final Research Achievements |
Novel E-cadherin-based engineered extracellular matrix showed fascinating results: (1) highly homogeneous proliferation and differentiation of definitive endoderm cells under single-cell level; (2)striking effect of matrix-dependent cell sorting for isolation and enrichment of mature hepatocytes and neural cells for possible elimination of contaminated and poorly differentiated cells; (3) the unique opportunity for continuous monitoring of cellular behavior in different stages of differentiation.
Taken together, our novel recombinant ECM is advantageous for generating homogeneous population of differentiated cells without any enzymatic stress and cell sorting as well as proliferation. We established a novel biomedical field in cadherin biology named as “Cadherin-Matrix Engineering” which can be applied to “Cell-cooking Plate” for ES/iPS cells.
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| Assessment Rating |
Verification Result (Rating)
A-
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Assessment Rating |
Result (Rating)
A-: Progress in the research is steadily towards the initial goal. However, further efforts are necessary as a part of the research progress is delayed.
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