Systems engineering approach for colitis remission by use of gut commensal bacteria

• Project/Area Number: 23360373
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Waseda University
• Principal Investigator: TSUNEDA SATOSHI 早稲田大学, 理工学術院, 教授 (30281645)
• Co-Investigator(Kenkyū-buntansha): 大坂 利文 早稲田大学, 付置研究所, 助教 (70514470) ; 加川 友己 早稲田大学, 付置研究所, 講師 (90409649)
• Co-Investigator(Renkei-kenkyūsha): OSAKA Toshifumi 早稲田大学, 理工学研究所, 招聘研究員 (70514470) ; KAGAWA Yuki 早稲田大学, ナノ理工学研究機構, 研究員 (90409649)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 腸内細菌 / プロバイオティクス / 炎症性疾患 / 代謝産物 / 大腸炎 / メタボローム / 数理モデル / 抗炎症

Outline of Final Research Achievements

We identified gut commensal bacteria retaining anti-inflammatory or epithelial tight junction barrier-strengthening properties by in vitro screening. Also, we characterized gut bacteria and metabolites retaining the potential to involve in colitis remission by using a dextran sulfate sodium-induced colitis mouse model. Furthermore, we developed the computational framework for elucidation of stem cell dynamics occurring in colonic crypts and quantitatively prediction of cell behavior.

Research Products

• [Journal Article] Strengthening of the intestinal epithelial tight junction by Bifidobacterium bifidum (2015)
  • Author(s): Chen-Yu Hsieh, Toshifumi Osaka, Eri Moriyama1, Yasuhiro Date, Jun Kikuchi, Satoshi Tsuneda
  • Journal Title: Physiological Reports Volume: 3 Issue: 3 Pages: e12327-e12327
  • DOI: 10.14814/phy2.12327
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Modeling of stem cell dynamics in human colonic crypts in silico (2014)
  • Author(s): Yuki Kagawa, Noriko Horita, Hideki Taniguchi, Satoshi Tsuneda
  • Journal Title: Journal of Gastroenterology Volume: 49 Issue: 2 Pages: 263-269
  • DOI: 10.1007/s00535-013-0887-x
  • Peer Reviewed
• [Presentation] The physiological importance of commensal Enterobacteriaceae populations in the gut homeostasis (2015)
  • Author(s): 大坂利文, 常田聡
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 岐阜
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] 腸内細菌代謝産物を介した大腸上皮細胞の炎症応答抑制 (2014)
  • Author(s): 大坂利文，謝鎮宇，森山恵里，伊達康博，菊地淳，常田聡
  • Organizer: 第66回日本生物工学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] DSS腸炎の寛解誘導に関わる腸管内細菌代謝物動態の解析 (2014)
  • Author(s): 森山恵里, 大坂利文, 伊達康博, 菊地淳, 常田聡
  • Organizer: 第66回日本生物工学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] 腸内細菌代謝産物を介した大腸上皮細胞の炎症応答抑制機構の探索 (2014)
  • Author(s): 鎌田瑞翔，大坂 利文，常田聡
  • Organizer: 第18回腸内細菌学会
  • Place of Presentation: 東京
  • Year and Date: 2014-06-11 – 2014-06-12
• [Presentation] 腸内細菌による腸管上皮バリア機能の強化・修復 (2014)
  • Author(s): 大坂利文，謝鎮宇，森山恵里，伊達康博，菊地淳，常田聡
  • Organizer: 第18回腸内細菌学会
  • Place of Presentation: 東京
  • Year and Date: 2014-06-11 – 2014-06-12
• [Presentation] Host-microbes interplay involving in the resolution of intestinal inflammation (2012)
  • Author(s): 大坂利文
  • Organizer: 14th International Symposium on Microbial Ecology
  • Place of Presentation: コペンハーゲン
• [Presentation] 大腸上皮細胞を用いた炎症応答抑制に関わる腸内細菌の探索 (2012)
  • Author(s): 松浦諒
  • Organizer: 第16回腸内細菌学会
  • Place of Presentation: 神戸
• [Presentation] DSS腸炎マウスの病態形成過程における腸内細菌由来TLRs刺激能の動態 (2012)
  • Author(s): 大坂利文
  • Organizer: 第16回腸内細菌学会
  • Place of Presentation: 神戸
• [Presentation] 腸内細菌叢が大腸炎の病態に与える影響解析 (2011)
  • Author(s): 三木硬介, 大坂利文, 伊達康博, 菊地淳, 常田聡
  • Organizer: 第27回日本微生物生態学会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2011-10-08
• [Presentation] 腸炎の病態形成に寄与する腸内環境因子の探索 (2011)
  • Author(s): 三木硬介, 大坂利文, 伊達康博, 菊地淳, 常田聡
  • Organizer: 平成23年度日本生物工学会大会
  • Place of Presentation: 東京農工大(東京都)
  • Year and Date: 2011-09-26
• [Presentation] Characterization of Colonic Microflora and Innate Immune-Stimulatory Activity during Colitis Progression (2011)
  • Author(s): T.Osaka, J.Hayasaki, K.Miki, S.Tsuneda
  • Organizer: International Union of Microbiological Societies Congresses 2011
  • Place of Presentation: 札幌国際会議場(北海道)
  • Year and Date: 2011-09-08
• [Presentation] In vitro Screening of Prospective Bacteria Modulating the Epithelial Inflammatory Response (2011)
  • Author(s): T.Osaka, R.Matsuura, S.Shibata, S.Tsuneda
  • Organizer: International Union of Microbiological Societies Congresses 2011
  • Place of Presentation: 札幌国際会議場(北海道)
  • Year and Date: 2011-09-08
• [Presentation] The interaction between commensal bacteria and innate immune receptors for modulating the intestinal inflammatory response
  • Author(s): Toshifumi Osaka, Chihiro Wayu, Junki Hayasaki, Kosuke Miki, Satoshi Tsuneda
  • Organizer: Cell Symposia: Microbiome and Host Health (CMIC2013)
  • Place of Presentation: リスボン
• [Presentation] 腸内細菌代謝産物を介した大腸上皮細胞の炎症応答抑制
  • Author(s): 大坂利文, 松浦諒, 常田聡
  • Organizer: 第17回腸内細菌学会
  • Place of Presentation: 東京
• [Presentation] 腸管上皮バリア機能の強化・修復を担う腸内細菌の探索
  • Author(s): 大坂利文，謝鎮宇，森山恵里, 伊達康博, 菊地淳, 常田聡
  • Organizer: 第19回Hindgut Club Japan
  • Place of Presentation: 東京
• [Presentation] The Regulation of the Intestinal Epithelial Tight Junction Protein by Bifidobacterium bifidum
  • Author(s): 謝鎮宇，大坂利文，常田聡
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張
• [Presentation] 腸炎の病態形成に関わる腸内環境因子の探索
  • Author(s): 森山恵里
  • Organizer: 第18回Hindgut Club Japan
  • Place of Presentation: 東京
• [Presentation] 腸管炎症制御に関わる細菌－自然免疫細胞の相互作用の解明
  • Author(s): 和湯千紘
  • Organizer: 第18回Hindgut Club Japan
  • Place of Presentation: 東京