Regulatory mechanisms of intracellular trafficking of Gq protein-coupled receptors
| Project/Area Number |
23590119
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Meiji Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
FUKUI Hiroyuki 徳島大学大学院, HBS研究部, 教授 (90112052)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ヒスタミンH1受容体 / Gq蛋白質共役型受容体 / C末端 / 細胞内輸送 / クラスリン / G蛋白質共役型受容体 / N末端 / プロテアソーム / Down-regulation / Internalization / リソソーム / ユビキチン / カベオラ |
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| Outline of Final Research Achievements |
We evaluated regulatory mechanisms of agonist-induced internalization of Gq protein-coupled human histamine H1 receptors by mutation analyses of the N- and C-terminals of receptors expressed in Chinese hamster ovary cells. H1 receptors tagged with hemagglutinin at the N-terminal internalized via clathrin-dependent mechanisms upon stimulation with histamine. In contrast, H1 receptors failed to internalize by truncation of the serine 487 residue of the C-terminal. These results suggest that the C-terminal of H1 receptors plays a crucial role in agonist-induced and clathrin-dependent internalization.
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Report
(5 results)
Research Products
(19 results)
