The functions of a new WD-repeat domain containing protein naofen to modulate LPS-induced liver dysfunction
| Project/Area Number |
23592689
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Aichi Medical University |
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Principal Investigator |
FENG Guo-Gang 愛知医科大学, 医学部, 講師 (70351111)
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Takashi 名古屋大学, 医学(系)研究科(研究院), 特任助教 (10378052)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | エンドトキシン(LPS) / クッパー細胞ptosis / TNFα / caspase-3 / アポトーシス / Bcl-2 / Bcl-xL / cytochrome c / LPS / クッパー細胞、 / bupivacaine / NF-κB / エンドトキシン / クッパー細胞 / プロポフォール / WD-repeat protein / チトクロムｃ |
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| Research Abstract |
Endothxin (LPS) causes hepatocytic apoptosis and liver dysfunction, as described in cases of sepsis. In this study, we therefore investigated the functions of a new WD-repeat domain containing protein naofen (WDR35) in LPS-induced liver dysfunction. LPS activated Kuppfer cells and released cytokines, such as TNF-alpha. Which then increased mRNA and protein expression of naofen in hepatocytes. Naofen/WDR35 inhibited expressions of anti-apoptotic proteins Bcl-2 and Bcl-xL, and stimulated apoptosis of hepatocytes. These results suggested that naofen may be involved in endotoxin-induced liver injury, and as a new therapeutic targets for endotoxin-induced liver injury therapy.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Comparison of the efficacy of ribavirin plus peginterferon alfa-2b for chronic hepatitis C infection in patients with and without coagulation disorders.2013
Author(s)
Honda T, Katano Y, Kuzuya T, Hayashi K, Ishigami M, Itoh A, Hirooka Y, Nakano I, Ishikawa T, Toyoda H, Kumada T, Yamamoto K, Matsushita T, Kojima T, Takamatsu J, Goto H
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Journal Title
J Med Virol.
Volume: 85(2)
Issue: 2
Pages: 228-234
DOI
Related Report
Peer Reviewed
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[Journal Article] Pegylated interferon monotherapy in patients with chronic hepatitis C with low viremia and its relationship to mutations in the NS5A region and the single nucleotide polymorphism of interleukin-28B.2013
Author(s)
Hayashi K, Katano Y, Masuda H, Ishizu I, Kuzuya T, Honda T, Ishigami M, Itoh A, Hirooka Y, Nakano I, Ishikawa T, Urano F, Yoshioka K, Toyoda H, Kumada T, Goto H.
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Journal Title
Hepatol Res
Volume: 43
Issue: 6
Pages: 580-588
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Prevalence of hepatitis C virus genotype 1a in Japan and correlation of mutations in the NS5A region and single-nucleotide polymorphism of interleukin-28B with the response to combination therapy with pegylated-interferon-alpha 2b and ribavirin.2012
Author(s)
Hayashi K, Katano Y, Kuzuya T, Tachi Y, Honda T, Ishigami M, Itoh A, Hirooka Y, Ishikawa T, Nakano I, Urano F, Yoshioka K, Toyoda H, Kumada T, Goto H.
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Journal Title
J Med Virol.
Volume: 84
Issue: 3
Pages: 438-44
DOI
Related Report
Peer Reviewed
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[Journal Article] Role of naofen in apoptosis of hepatocytes induced by lipopolysaccharide through mitochondrial signaling in rats.2012
Author(s)
Fan JH, Feng GG, Huang L, Tsunekawa K, Honda T, Katano Y, Hirooka Y, Goto H, Kandatsu N, Ando K, Fujiwara Y, Koide T, Okada S, Ishikawa N.
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Journal Title
Hepatol Res.
Volume: 42
Issue: 7
Pages: 696-705
DOI
NAID
Related Report
Peer Reviewed
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