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Analyses of cell-cycle dependency of intracellular events induced by RNAs

Research Project

Project/Area Number 23651221
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Living organism molecular science
Research InstitutionOkayama University

Principal Investigator

OHTSUKI Takashi  岡山大学, 大学院・自然科学研究科, 教授 (80321735)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsバイオテクノロジー / 核酸 / 細胞分化 / RNA / 細胞周期 / 分化 / 発生・分化
Research Abstract

Cell cycle dependency of RNAi was analyzed by introducing RNA into cell cycle synchronized cells and by introducing RNA into cells, in which a specific cell cycle was visualized. As a result, cell cycle dependency of RNAi was shown, but reproducibility of the result should be confirmed In addition, to examine cell cycle dependency of cell differentiation induced by RNA, we established a method to promote muscular cell differentiation promotion by introducing the RNA into cells.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (26 results)

All 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (17 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Synthesis and Properties of Peptide Dendrimers Containing Fluorescent and Branched Amino Acids. Biopolymers2013

    • Author(s)
      Kitamatsu, M., Kitabatake, M., Noutoshi, Y.,Ohtsuki, T.
    • Journal Title

      Peptide Science

      Volume: 100 Issue: 1 Pages: 64-70

    • DOI

      10.1002/bip.22175

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photoinduced RNA Interference2012

    • Author(s)
      Matsushita-Ishiodori Y, Ohtsuki T
    • Journal Title

      Accounts of Chemical Research

      Volume: (Epub ahead of print) Issue: 7 Pages: 1039-1047

    • DOI

      10.1021/ar200227n

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photosensitizing carrier proteins for photoinducible RNA interference2011

    • Author(s)
      Matsushita-Ishiodori, Y., Kuwabara, R., Sakakoshi, H., Endoh, T., Ohtsuki, T.
    • Journal Title

      Bioconjugate Chemistry

      Volume: 22 Issue: 11 Pages: 2222-2226

    • DOI

      10.1021/bc200095a

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Synthesis and in situ insertion of a site-specific fluorescently labeled membrane protein into cell-sized liposomes2011

    • Author(s)
      T. Ohtsuka, S. Neki, T. Kanai, K. Akiyoshi, S. M. Nomura, T. Ohtsuki
    • Journal Title

      Analytical Biochemistry

      Volume: 418 Issue: 1 Pages: 97-101

    • DOI

      10.1016/j.ab.2011.06.026

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 可視光照射によるRNAiの時空間的制御法 (CLIP-RNAi法)2011

    • Author(s)
      石躍由佳,遠藤玉樹,大槻高史
    • Journal Title

      実験医学

      Volume: 29 Pages: 1297-1302

    • Related Report
      2011 Research-status Report
  • [Presentation] Spatial regulation of specific gene expression through photoactivation of RNAi2013

    • Author(s)
      Takashi Ohtsuki
    • Organizer
      Taiwan-Japan Manomedicine Meeting
    • Place of Presentation
      Academia Sinica, Taipei
    • Related Report
      2012 Final Research Report
  • [Presentation] Spatial regulation of specific gene expression through photoactivation of RNAi2013

    • Author(s)
      Takashi Ohtsuki
    • Organizer
      Taiwan-Japan Manomedicine Meeting
    • Place of Presentation
      Taipei
    • Related Report
      2012 Annual Research Report
  • [Presentation] 可視光および近赤外光による細胞内RNA導入の誘導2012

    • Author(s)
      大槻高史、松本祥、遠藤玉樹、石躍由佳
    • Organizer
      日本バイオマテリアル学会九州ブロック第2回九州地区講演会
    • Place of Presentation
      福岡
    • Year and Date
      2012-09-14
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] CLIP-RNAi法におけるキャリア・RNAの安定性の検討2012

    • Author(s)
      松本 祥,石躍 由佳、大槻 高史
    • Organizer
      第53回日本生化学会中国・四国支部例会
    • Place of Presentation
      岡山
    • Year and Date
      2012-05-18
    • Related Report
      2012 Final Research Report
  • [Presentation] Spatial regulation of specific gene expression through photoactivation of RNAi2012

    • Author(s)
      Takashi Ohtsuki, Yuka Matsushita-Ishiodori, Tamaki Endoh
    • Organizer
      International Joint Symposium on Single-Cell Analysis
    • Place of Presentation
      Kyoto
    • Related Report
      2012 Final Research Report
  • [Presentation] 近赤外光及び可視光を用いたCLIP-RNAi2012

    • Author(s)
      松本 祥、石躍 由佳、大槻 高史
    • Organizer
      第6回バイオ関連化学シンポジウム
    • Place of Presentation
      札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞内noncoding RNA局在追跡ツールの開発2012

    • Author(s)
      田村佳史, 渡邉和則, 大槻高史
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] Spatial regulation of specific gene expression through photoactivation of RNAi2012

    • Author(s)
      Takashi Ohtsuki, Yuka Matsushita-Ishiodori, Tamaki Endoh
    • Organizer
      International Joint Symposium on Single-Cell Analysis
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report
  • [Presentation] 近赤外光及び可視光を用いたCLIP-RNAi2012

    • Author(s)
      松本 祥、石躍 由佳、大槻 高史
    • Organizer
      第 6回バイオ関連化学シンポジウム
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] CLIP-RNAi法における新規RNAキャリアタンパク質の開発2011

    • Author(s)
      石躍由佳、桑原里奈、遠藤玉樹、大槻高史
    • Organizer
      第52回日本生化学会 中国・四国支部例会
    • Place of Presentation
      広島
    • Year and Date
      2011-05-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞内non-coding RNA局在追跡ツールの開発2011

    • Author(s)
      田村佳史, 石躍由佳, 大槻高史
    • Organizer
      日本化学会西日本大会
    • Place of Presentation
      徳島
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] Design of RNA carrier molecules for photoinduced RNA interference2011

    • Author(s)
      Yuka Matsushita-Ishiodori, Rina Kuwabara, Hiroyuki Sakakoshi, Tamaki Endoh and Takashi Ohtsuki
    • Organizer
      38th International Symposium on Nucleic Acid Chemistry
    • Place of Presentation
      Sapporo
    • Related Report
      2012 Final Research Report
  • [Presentation] 近赤外光による照射領域特異的RNAi誘導法の開発2011

    • Author(s)
      石躍由佳,森長美香,大槻高史
    • Organizer
      第84回 日本生化学会大会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] 光応答性キャリア蛋白質を用いたRNAデリバリー2011

    • Author(s)
      大槻 高史, 松本 祥,遠藤 玉樹,石躍 由佳
    • Organizer
      アンチセンス・遺伝子・デリバリー シンポジウム
    • Place of Presentation
      大阪
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] CLIP-RNAi法によるRNAiの光誘導2011

    • Author(s)
      大槻高史, 米澤政樹,松本祥,遠藤玉樹,石躍由佳
    • Organizer
      第33回日本光医学・光生物学会
    • Place of Presentation
      大阪
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] Design of RNA carrier molecules for photoinduced RNA interference.2011

    • Author(s)
      Yuka Matsushita-Ishiodori, Rina Kuwabara, Hiroyuki Sakakoshi, Tamaki Endoh and Takashi Ohtsuki
    • Organizer
      38th International Symposium on Nucleic Acid Chemistry
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report
  • [Presentation] 細胞内noncoding RNA局在追跡ツールの開発

    • Author(s)
      田村佳史, 渡邉和則,大槻高史
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Book] クローズアップ実験法」可視光照射によるRNAiの時空間的制御法 (CLIP-RNAi法)2011

    • Author(s)
      石躍由佳,遠藤玉樹,大槻高史
    • Publisher
      実験医学5月号
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.biotech.okayama-u.ac.jp/labs/ohtsuki/index.htm

    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 近赤外光によりRNAを細胞質内に送達するための新規なキャリア分子ならびに方法2011

    • Inventor(s)
      大槻高史、石躍由佳
    • Industrial Property Rights Holder
      大槻高史、石躍由佳
    • Filing Date
      2011-11-24
    • Related Report
      2012 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 細胞内へ核酸を導入する為の新規な分子並びに細胞内へ導入する核酸および細胞内へ核酸を導入する為の新規な方法2011

    • Inventor(s)
      川井淳、川上文清、大槻高史、宍戸昌彦
    • Industrial Property Rights Holder
      川井淳、川上文清、大槻高史、宍戸昌彦
    • Acquisition Date
      2011-04-01
    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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