Research and development toward the determination for the redox state of individual metal atoms in proteins

• Project/Area Number: 23657073
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Kyoto University
• Principal Investigator: TAKEDA Kazuki 京都大学, 大学院・理学研究科, 講師 (30332290)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 異常分散 / 金属 / タンパク質 / 異常分散効果

Research Abstract

Research and development toward the determination for the redox states of individual metal atoms in proteins from X-ray diffraction data sets collected near the absorption edges of the metals were performed by using the high-potential iron sulfur protein having the 4Fe-4S cluster and the Rieske protein having the 2Fe-2S cluster.

Research Products

• [Journal Article] Structure of a thermophilic cyanobacterial b6f-type Rieske protein (2012)
  • Author(s): Veit, S., Takeda, K., Tsunoyama, Y., Rexroth, D., Roegner, M. and Miki, K.
  • Journal Title: Acta cryst. Volume: D68 Issue: 10 Pages: 1400-1408
  • DOI: 10.1107/s0907444912034129
  • Peer Reviewed
• [Journal Article] Ultra-high Resolution Protein Crystallography (2011)
  • Author(s): 竹田一旗、三木邦夫
  • Journal Title: Seibutsu Butsuri Volume: 51 Issue: 2 Pages: 096-097
  • DOI: 10.2142/biophys.51.096
  • NAID: 10028105855
  • ISSN: 0582-4052, 1347-4219
• [Journal Article] Dimer structure and conformational variability in the N-terminal region of an archaeal small heat shock protein, StHsp14.0 (2011)
  • Author(s): Takeda K, Hayashi T, Abe T, Hirano Y Hanazono Y, Yohda M, Miki K.
  • Journal Title: J Struct Biol Volume: 174 Issue: 1 Pages: 92-99
  • DOI: 10.1016/j.jsb.2010.12.006
  • Peer Reviewed
• [Journal Article] Dimer structure and conformational variability in the N-terminal region of an archaeal small heat shock protein, StHsp14.0 (2011)
  • Author(s): K. Takeda, T. Hayashi, T. Abe, Y. Hirano, Y. Hanazono, M. Yohda, K. Miki
  • Journal Title: J. Struct. Biol. Volume: 174 Pages: 92-99
  • Peer Reviewed
• [Remarks]
  • URL: http://www.kuchem.kyoto-u.ac.jp/organization/member/ktakeda.html