Engineering of monomer supplying enzyme for enhanced biopolymer production
| Project/Area Number |
23710102
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Hokkaido University |
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Principal Investigator |
WATANABE Tsuyoshi 北海道大学, 大学院・工学研究院, 博士研究員 (40599601)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | アセトアセチル CoA レダクターゼ / アセトアセチル-CoAレダクターゼ / ３ヒドロキシアルカン酸ベースポリマー / 進化工学 |
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| Research Abstract |
Acetoacetyl-CoA reductase (PhaB) catalyzes the reduction of acetoacetyl-CoA into 3-hydroxubutyryl-CoA using NADPH as a reducing power.3-Hydroxybutyryl-CoA serves as a monomer substrate for the microbial polyester, polyhydroxybutyrate. The aim of this study is to engineer PhaB for enhancing the enzymatic activity. The kinetic analysis of the selected mutants revealed that they possess enhanced turnover rate. The effectiveness of the obtained mutants of PhaB on polyhydroxybutyrate production was evaluated using Corynebacterium glutamicum, indicating that the content of polyhydroxybutyrate was increased up to 3-fold compared to the wild-type PhaB.
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Report
(3 results)
Research Products
(8 results)
