• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Transposon mutagenesis of probiotic Lactobacillus casei identifies asnH, an asparagine synthetase gene that contributes to activation of host innate immunity

Research Project

Project/Area Number 23790485
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bacteriology (including Mycology)
Research InstitutionKitasato University

Principal Investigator

ITO Masahiro  北里大学, 薬学部, 助教 (90596727)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsプロバイオティクス / 自然免疫 / 菌体表層構造 / 自然免疫賦活化
Research Abstract

We demonstrated that AsnH exhibits asparagine synthetase activity when the lysozyme-treated cell wall extract of L. casei was added as substrate. L. caseicarrying mutated asnH lacked its characteristic thick and rigid cell wall structure, indicating that AsnH of L. casei is involved in cell wall biosynthesis. These results indicate that asnH is required for bacterial cell wall biosynthesis and is involved in the probiotic activity of L. casei ATCC 27139.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (2 results)

All 2013

All Presentation (2 results)

  • [Presentation] Transposon mutagenesis of probiotic Lactobacillus casei identifies asnH, an asparagine synthetase gene that contributes to activation of host innate immunity2013

    • Author(s)
      Masahiro Ito
    • Organizer
      Exploring Human Host-Microbiome Interactions in Health and Disease 2013
    • Place of Presentation
      Cambridge (UK)
    • Year and Date
      2013-07-10
    • Related Report
      2012 Final Research Report
  • [Presentation] Asparagine Synthetase Plays an Important Role in Enhancement of Host Innate Immunity by Probiotic Lactobacillus casei2013

    • Author(s)
      Masahiro Ito, Yun-Gi Kim, Hirokazu Tsuji, Takuya Takahashi, Mayumi Kiwaki, Koji Nomoto, Hirofumi Danbara, Nobuhiko Okada
    • Organizer
      The 2nd Wellcome Trust conference on Exploring Human Host-Microbiome Interactions in Health and Disease
    • Place of Presentation
      Fitzwilliam College, Cambridge, UK
    • Related Report
      2012 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi