| Project/Area Number |
23791665
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Showa University |
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Principal Investigator |
SATO Atsushi 昭和大学, 医学部, 助教 (10445596)
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| Research Collaborator |
OHTAKI Hirokazu 昭和大学, 助教 (20349062)
SHIODA Seiji 昭和大学, 教授 (80102375)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 脊髄損傷 / 神経炎症 / マイクログリア / マクロファージ / インターロイキン１ / 骨髄間葉系幹細胞 / 代替経路型活性化型 / 古典的活性化型 / ヒト骨髄間葉系幹細胞 / PACAP / IL-1 / 炎症応答 |
|---|
| Research Abstract |
Microglia and macrophages (MG/MF) have a diverse range of functions depending on unique cytokine stimuli, and contribute to neural cell death, repair, and remodeling during central nervous system diseases. While IL-1 has been shown to exacerbate inflammation, it has also been recognized to enhance neurodegeneration. We determined the activating phenotype of MG/MF and the impact of IL-1 in an in vivo spinal cord injury (SCI) model of IL-1 knock-out (KO) mice. Moreover, we demonstrated the contribution of IL-1 to both the classical and alternative activation of MG in vitro using an adult MG primary culture.
We demonstrate here in in vivo experiments that IL-1 suppressed SCI in a process mediated by the reduction of inflammatory responses. Moreover, we suggest that IL-1 participates in both the classical and alternative activation of MG in in vivo and in vitro systems. hMSCs Implanted to injured spinal cord have been recognized to improve motor function, neuroinflammation and regeneration.
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