Molecular mechanism of the differentiation control by the mechanical stimulation of the mesenchymal cells which is in the process of chondrocyte differentiation

• Project/Area Number: 23792414
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: KUMIKO Kosaka 東北大学, 大学院・歯学研究科, 大学院非常勤講師 (30585336)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 機械的刺激 / 軟骨細胞分化 / MAPK

Research Abstract

In this study, I found that mechanical compressive stimulation promoted chondrogenic differentiation of mesenchymal cells derived from rat lim bud but stretch stimulation inhibited it. In addition, phosphorylation and nuclear translocation of ERK-1/2 were observed after mechanical stretch stimulation. Therefore, activation of ERK-1/2 pathway by mechanical stimulation is involved in mechano-sensing signaling pathway of chondrogenesis.