Health assessment of PAMAM dendrimer with a spherical dendritic structure
| Project/Area Number |
24241013
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental impact assessment/Environmental policy
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| Research Institution | National Institute for Environmental Studies |
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Principal Investigator |
FUKUDA HIDEKO 独立行政法人国立環境研究所, 環境リスク研究センター, 室長 (60280715)
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| Co-Investigator(Kenkyū-buntansha) |
KUROKAWA Yoshika 国立環境研究所, 環境リスク研究センター, 主任研究員 (30205231)
WIN-SHWE TINTIN 国立環境研究所, 環境健康研究センター, 主任研究員 (00391128)
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| Research Collaborator |
HIRANO Seishiro 国立環境研究所, 環境リスク研究センター
ZENG Yang 国立環境研究所, 環境リスク研究センター
MATSUI Yasuto 京都大学, 大学院工学研究科
KIMURA Hiroyuki 京都大学, 大学院薬学研究科
YAMAZAKI Masashi 産業技術総合研究所
HAYAMA Kazumi 産業技術総合研究所
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥47,710,000 (Direct Cost: ¥36,700,000、Indirect Cost: ¥11,010,000)
Fiscal Year 2014: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2013: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2012: ¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000)
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| Keywords | ナノマテリアル / 環境リスク / 健康 / 生体影響評価 / in vitro / 細胞 / 毒性 / 脳 / デンドリマー / 化学物質管理 / 樹状分子 / 点鼻投与 / ナノ毒性 / 共焦点レーザー顕微鏡 / 組織 / 動態 |
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| Outline of Final Research Achievements |
We examined the potential adverse effect of PD by using physico-chemical analysis and in vivo/in vitro exposure tests. Monitoring particle size in various liquid revealed that PD showed rapid aggregation in the presence of divalent cations. The aggregates became bigger than the submicron size in culture media or the standard river water. Animal experiments showed that intranasal instillation of PD caused changes in mRNA expression level of neurological markers in the brain tissues. However, we did not find translocation of fluorescent-labeled or radio-labeled PD after intranasal or intravenous administration. Moreover, we also found that exposure to PD of different surface amino groups dose-dependently inhibited cellular proliferation and differentiation in human neural precursor cell. Our results suggest that PDs aggregate in liquid environments, but exposure to PD causes cellular toxicity and alteration of gene expression in neural cells and nervous tissues.
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Report
(4 results)
Research Products
(16 results)
