• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Search for drugs targeted against the LYAR cell growth factor

Research Project

Project/Area Number 24241075
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemical biology
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Takahashi Nobuhiro  東京農工大学, (連合)農学研究科(研究院), 教授 (80293017)

Co-Investigator(Kenkyū-buntansha) MIURA Yutaka  東京農工大学, 大学院農学研究院, 教授 (10219595)
IZUMIKAWA Keiichi  東京農工大学, 大学院農学府, 特任助教 (60625713)
ISHIKAWA Hideaki  東京農工大学, 大学院農学府, 特任助教 (80625715)
YOSHIKAWA Harunori  東京農工大学, 大学院農学府, 特任助教 (60709567)
ISOBE Toshiaki  首都大学東京, 大学院理工学研究科, 特任教授 (70106607)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥43,030,000 (Direct Cost: ¥33,100,000、Indirect Cost: ¥9,930,000)
Fiscal Year 2015: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2014: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2013: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2012: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Keywordsケミカルバイオロジー / プロテオミクス / 医薬品探査 / がん / 機能プロテオミクス / ガン治療 / 転写抑制 / タンパク質相互作用 / 抗がん薬 / エピジェンティクス / リボソームRNAプロセシング / エピジェンティック / リボソームRNAのプロセシング / エピジェネティクス
Outline of Final Research Achievements

The cancer cells can grow indefinitely and quickly by increasing the efficiency of ribosome biogenesis that use the 60-80% of the energy and substances for which the cells are required. By inhibiting the action of a factor that increases the efficiency of the ribosome biogenesis in cancer cells, it is expected to suppress the proliferation of cancer cells. This study, based on the finding that LYAR is a such factor, has attempted to develop methods of screening for compounds that inhibit the action of LYAR. By analyzing the molecular mechanisms by which LYAR promote ribosome biogenesis, we identified a new factor that bind to LYAR without any co-factor between them. Unexpectedly, BRD4 was not the one that bind to directly to LYAR. It has become possible to develop a screening system of a new cancer treatment drugs based on the results.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (36 results)

All 2016 2015 2014 2013 2012 Other

All Int'l Joint Research (2 results) Journal Article (10 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 10 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results) Presentation (20 results) (of which Int'l Joint Research: 1 results,  Invited: 4 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Int'l Joint Research] La Trobe University/Trobe Institute for Molecular Science(オーストラリア)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] University of Dundee/Centre for Gene Regulation & Expression/College of Life Sciences(英国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Extracellular vesicle isolation and characterization: towards clinical application.2016

    • Author(s)
      Rong Xu, David Greening, Nobuhiro Takahashi, Richard Simpson
    • Journal Title

      Journal of Clinical Investigation

      Volume: 126 Issue: 4 Pages: 1152-1162

    • DOI

      10.1172/jci81129

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A mass spectrometry-based method for comprehensive quantitative determination of post-transcriptional RNA modifications: the complete chemical structure of Schizosaccharomyces pombe ribosomal RNAs2015

    • Author(s)
      Taoka M, Nobe Y, Hori M, Takeuchi A, Masaki S, Yamauchi Y, Nakayama H, Takahashi N, IsobeT.
    • Journal Title

      Nucleic Acids Res.

      Volume: 43 Issue: 18 Pages: e115-e115

    • DOI

      10.1093/nar/gkv560

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Human nucleolar protein Nop52 (RRP1/NNP-1) is involved in site 2 cleavage in internal transcribed spacer 1 of pre-rRNAs at early stages of ribosome biogenesis.2015

    • Author(s)
      Yoshikawa H, Ishikawa H, Izumikawa K, Miura Y, Hayano T, Isobe T, Simpson RJ, Takahashi N.
    • Journal Title

      Nucleic Acids Res.

      Volume: 43 Issue: 11 Pages: 5524-5536

    • DOI

      10.1093/nar/gkv470

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Collaborator of alternative reading frame protein (CARF) regulates early processing of pre-ribosomal RNA by retaining XRN2 (5’-3’ exoribonuclease) in the nucleoplasm.2015

    • Author(s)
      Sato+, S., Ishikawa+, H., Yoshikawa, H., Izumikawa, K., Simpson, R.J. and Takahashi, N.
    • Journal Title

      Nucleic Acids Res.

      Volume: 43 Pages: 10397-10397

    • DOI

      10.1093/nar/gkv1069

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Comprehensive Analysis of RNA–Protein Complex Using Mass Spectrometry-Based Technology2015

    • Author(s)
      泉川桂一、石川英明、吉川治孝、礒辺俊明、高橋信弘
    • Journal Title

      KAGAKU TO SEIBUTSU

      Volume: 53 Issue: 12 Pages: 850-859

    • DOI

      10.1271/kagakutoseibutsu.53.850

    • NAID

      130005284426

    • ISSN
      0453-073X, 1883-6852
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Human nucleolar protein Nop52 (RRP1/NNP-1) is involved in site 2 cleavage in internal transcribed spacer 1 of pre-rRNAs at early stages of ribosome biogenesis.2015

    • Author(s)
      Yoshikawa H., Ishikawa H., Izumikawa K., Miura Y., Hayano T., Isobe T., Simpson R.J. and Takahashi N.
    • Journal Title

      Nucleic Acids Research

      Volume: in press

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Global PROTOMAP Profiling to Search for Biomarkers of Early-Recurrent Hepatocellular Carcinoma2014

    • Author(s)
      Taoka, Masato; Morofuji, Noriaki; Yamauchi, Yoshio; Ojima, Hidenori; Kubota, Daisuke; Terukina, Goro; Nobe, Yuko; Nakayama, Hiroshi; Takahashi, Nobuhiro; Kosuge, Tomoo; Isobe, Toshiaki; Kondo, Tadashi
    • Journal Title

      J. Proteome Res.

      Volume: 13 Issue: 11 Pages: 4847-4858

    • DOI

      10.1021/pr500262p

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA cytidine acetyltransferase of small-subunit ribosomal RNA: identification of acetylation sites and the responsible acetyltransferase in fission yeast, Schizosaccharomyces pombe.2014

    • Author(s)
      Taoka M, Ishikawa D, Nobe Y, Ishikawa H, Yamauchi Y, Terukina G, Nakayama H, Hirota K, Takahashi N, Isobe T
    • Journal Title

      PLoS One

      Volume: 9 Issue: 11 Pages: e112156-e112156

    • DOI

      10.1371/journal.pone.0112156

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Human cell growth regulator Ly-1 antibody reactive homolog accelerates processing of pre-ribosomal RNA2014

    • Author(s)
      Miyazawa N., Yoshikawa, H., Magae S., Ishikawa H., Izumikawa K., Terukina G., Suzuki A, Nakamura-Fujiyama S., Miura Y., Hayano T, Komatsu K., Isobe T, and Takahashi N.
    • Journal Title

      Genes to Cells

      Volume: 19 Issue: 4 Pages: 273-286

    • DOI

      10.1111/gtc.12129

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Large-scale identification of N-glycosylated proteins of mouse tissues and construction of a glycoprotein database, GlycoProtDB2012

    • Author(s)
      Kaji, H.,Shikanai, T., Sasaki-Sawa, A., Wen, H., Fujita, M., Suzuki, Y., Sugahara, D., Sawaki, H., Yamauchi, Y., Shinkawa, T., Taoka, M., Takahashi, N., Isobe, T., Narimatsu, H. (Kaji H.)
    • Journal Title

      Journal of Proteome Research

      Volume: 11 Issue: 9 Pages: 4553-4566

    • DOI

      10.1021/pr300346c

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Identification and quantification of RNA Post-transcriptional modifications by Nano-flow Liquid Chromatography-Tandem Mass Spectrometry and Database Searching2016

    • Author(s)
      H. Nakayama, M. Taoka, N. Takahashi, T. Isobe
    • Organizer
      Pittcon (Pittsburg conference) 2016
    • Place of Presentation
      Atlanta, USA
    • Year and Date
      2016-03-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 遺伝病原因遺伝子産物が形成するRNA-タンパク質複合体の機能解析2015

    • Author(s)
      高橋信弘
    • Organizer
      第63回プロテオーム医療創薬研究会
    • Place of Presentation
      横浜市立大学先端医科学研究センター
    • Year and Date
      2015-11-26
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Elucidation of RNP function in animal/plant cells, and development of its regulatory methods2015

    • Author(s)
      Takahashi N.
    • Organizer
      Grobal Inovation Research Organization Symposium
    • Place of Presentation
      Green Hall Building, Tokyo
    • Year and Date
      2015-11-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞増殖制御因子hLARのリボソーム生合成経路における機能解析2015

    • Author(s)
      泉川桂一、宮澤直樹、石川英明、吉川治孝、長橋花織、高橋信弘
    • Organizer
      第三回リボソームミーティング
    • Place of Presentation
      ANAホリデイ・イン リゾート 宮崎
    • Year and Date
      2015-03-17 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] プロテオミクスによるヒトのリボソーム合成過程の解析2015

    • Author(s)
      吉川治孝、石川英明、泉川桂一、高橋信弘
    • Organizer
      第三回リボソームミーティング
    • Place of Presentation
      ANAホリデイ・イン リゾート 宮崎
    • Year and Date
      2015-03-17 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] リボヌクレアーゼを介したヒトリボソーム生合成調節機構2015

    • Author(s)
      石川英明、佐藤慈子、泉川桂一、吉川治孝3、礒辺俊明、高橋信弘
    • Organizer
      第三回リボソームミーティング
    • Place of Presentation
      ANAホリデイ・イン リゾート 宮崎
    • Year and Date
      2015-03-17 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] プロトマップ解析によって発見された肝がん再発予測マーカー分子と肝がん再発メカニズム2014

    • Author(s)
      照喜名悟朗, 田岡万悟、山内芳雄、藤田千春、高橋信弘、近藤格、礒辺俊明
    • Organizer
      日本プロテオーム学会2014(JHUPO2014)
    • Place of Presentation
      つくば国際会議場、筑波
    • Year and Date
      2014-07-14 – 2014-07-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒト細胞増殖因子LYARは多種類の腫瘍細胞で高発現しておりリボソーム合成を昂進している2014

    • Author(s)
      吉川治孝、宮澤直樹、馬替恵美、石川英明、泉川桂一、照喜名悟朗、三浦豊、早野俊哉、礒辺俊明、渡辺亮、油谷裕幸、高橋信弘
    • Organizer
      日本プロテオーム学会2014(JHUPO2014)
    • Place of Presentation
      つくば国際会議場、筑波
    • Year and Date
      2014-07-14 – 2014-07-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Comprehensive mass spectrometry-based structural determination of small subunit ribosomal RNA: Characterization of N4-acetylcytidine and identification of the responsible enzyme.2014

    • Author(s)
      Masato Taoka; Daisuke Ishikawa; Yuko Nobe; Hideaki Ishikawa; Hiroshi Nakayama; Yoshio Yamauchi; Nobuhiro Takahashi; Toshiaki Isobe
    • Organizer
      62nd ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Baltimore Convention Center, USA
    • Year and Date
      2014-06-15 – 2014-06-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Snapshot analysis of protein complexes using proteomic technology (JHUPO Award Lectures)2013

    • Author(s)
      Takahashi N
    • Organizer
      HUPO 12th Annual Wold Congress-JHUPO 2013 Anual Joint Congress
    • Place of Presentation
      Yokohama
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Human cell growth regulator LYAR is highly expressed in many tumors and accelerates ribosome biogenesis.2013

    • Author(s)
      Izumikawa, K., Miyazawa, N., Yoshikawa, H., Ishikawa, H., Terukina, G., Miura, Y., Hayano, T., Isobe, T., Watanabe, A., Aburatani, H., Takahashi, N.
    • Organizer
      HUPO 12th Annual Wold Congress-JHUPO 2013 Anual Joint Congress
    • Place of Presentation
      Yokohama
    • Related Report
      2013 Annual Research Report
  • [Presentation] The Role of Nucleolar Protein Nop52 in the pre-rRNA Processing During Human Ribosome Biogenesis by Proteomic Approach2013

    • Author(s)
      Yoshikawa, H., Ishikawa, H., Izumikawa, K., Hayano, T., Miura, Y., Yamauchi, Y., Isobe, T., Takahashi, N.
    • Organizer
      HUPO 12th Annual Wold Congress-JHUPO 2013 Anual Joint Congress
    • Place of Presentation
      Yokohama
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒトリボソーム40Sサブユニット生合成におけるPARNタンパク質の役割2013

    • Author(s)
      石川英明、泉川桂一、吉川治孝、礒辺俊明、高橋信弘(高橋信弘)
    • Organizer
      第2回RIBOSOME MEETING
    • Place of Presentation
      東京、東京農工大学農学部
    • Related Report
      2012 Annual Research Report
  • [Presentation] 多種類の腫瘍細胞で高発現しているヒト細胞増殖因子LYARはリボソーム生合成を昂進している2012

    • Author(s)
      宮澤直樹、吉川治孝、馬替恵美、石川英明、泉川桂一、照喜名悟朗、鈴木愛、藤山-中村沙理1、三浦豊、早野俊哉、礒辺俊明、渡辺亮、油谷裕幸、高橋信弘(高橋信弘)
    • Organizer
      日本プロテオーム学会2012大会(10th JHUPO conference)
    • Place of Presentation
      東京、日本科学未来館
    • Related Report
      2012 Annual Research Report
  • [Presentation] 胃幽門毛細血管拡張症患者の自己抗体が認識するRNA helicase II/Guaのヒトリボソーム生合成における作用機序の解析2012

    • Author(s)
      安村大樹、吉川治孝、大嶋一史、Ben C. Valdez、高橋信弘(高橋信弘)
    • Organizer
      日本プロテオーム学会2012大会(10th JHUPO conference)
    • Place of Presentation
      東京、日本科学未来館
    • Related Report
      2012 Annual Research Report
  • [Presentation] 癌抑制因子Arfの相互作用タンパク質CARFの細胞増殖における新規機能2012

    • Author(s)
      佐藤慈子、石川英明、泉川桂一、高橋信弘(高橋信弘)
    • Organizer
      日本プロテオーム学会2012大会(10th JHUPO conference)
    • Place of Presentation
      東京、日本科学未来館
    • Related Report
      2012 Annual Research Report
  • [Presentation] プロテオミクスの手法を用いた核小体タンパク質Nop52のヒトリボソーム生合成における役割の解明2012

    • Author(s)
      吉川治孝、川崎実由希、早野俊哉、泉川桂一、石川英明、山内芳雄、礒辺俊明、高橋信弘(高橋信弘)
    • Organizer
      日本プロテオーム学会2012大会(10th JHUPO conference)
    • Place of Presentation
      東京、日本科学未来館
    • Related Report
      2012 Annual Research Report
  • [Presentation] ガン細胞に特異的なリボソーム合成促進因子をターゲットとした細胞増殖阻害物質探索法の開発

    • Author(s)
      泉川桂一、吉川治孝、石川英明、高橋信弘
    • Organizer
      東京バイオマーカー・イノベーション技術組合 TOBIRA研究交流フォーラム
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] リボヌクレオプロテオミクスによる疾患解析

    • Author(s)
      高橋信弘、石川英明、泉川桂一、中山洋、田岡万悟、礒辺俊明(高橋信弘)
    • Organizer
      第8回臨床プロテオーム研究会
    • Place of Presentation
      東京、都市センターホテル
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] プロテオミクスの手法を用いたヒト細胞におけるリボソーム生合成制御機構の解析

    • Author(s)
      高橋信弘(高橋信弘)
    • Organizer
      明治薬科大学ハイテクリサーチセンター公開セミナー
    • Place of Presentation
      東京、明治薬科大学ハイテクリサーチセンター
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Remarks] ガン細胞が増殖する原理を解明し、その根本的治療法を開発する研究

    • URL

      http://www.tuat.ac.jp/~ntakahas/Takahashi_Lab_-TAT-/Research.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] ガン細胞が増殖する原理を解明し、その根本的治療法を開発する研究

    • URL

      http://www.tuat.ac.jp/~ntakahas/Takahashi_Lab_-TAT-/Research.html

    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞増殖促進剤及び癌細胞増殖抑制剤の単離方法2016

    • Inventor(s)
      宮澤直樹、高橋信弘、泉川桂一、石川英明、吉川治孝
    • Industrial Property Rights Holder
      宮澤直樹、高橋信弘、泉川桂一、石川英明、吉川治孝
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-057432
    • Filing Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] TDP-43細胞内存在量関連疾患の認定方法2013

    • Inventor(s)
      泉川桂一、高橋信弘、他
    • Industrial Property Rights Holder
      東京農工大学・首都大学東京
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-02-28
    • Related Report
      2012 Annual Research Report
    • Overseas

URL: 

Published: 2013-05-15   Modified: 2022-02-07  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi