Detection of structural change of DNA using disulfide bond formation and its application to elucidation of molecular recognition mechanisms
| Project/Area Number |
24310158
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Osaka University |
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Principal Investigator |
IWAI Shigenori 大阪大学, 基礎工学研究科, 教授 (10168544)
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| Co-Investigator(Renkei-kenkyūsha) |
SUGASAWA Kaoru 神戸大学, バイオシグナル研究センター, 教授 (70202124)
KOJIMA Chojiro 大阪大学, 蛋白質研究所, 准教授 (50333563)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | ＤＮＡ / 構造変化 / ジスルフィド結合 / ＨＰＬＣ / タンパク質 / 分子認識 / DNA / HPLC |
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| Outline of Final Research Achievements |
To detect the DNA helix bending by a chemical approach, duplexes containing 2-O-mercaptoalkyl-β-D-arabinofuranose on both sides of the major groove were synthesized, and the effects of the cisplatin adduct, the abasic site analog, and the (6-4) photoproduct on DNA structures were investigated. A disulfide bond was formed depending on the cisplatin adduct, and dynamic DNA bending was shown for the abasic site and the (6-4) photoproduct. Using the bent structure obtained by this method, the model proposed for the handover of damaged DNA from the UV-DDB protein to the XPC protein in the nucleotide excision repair was validated.
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Report
(4 results)
Research Products
(3 results)
