• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Detection of structural change of DNA using disulfide bond formation and its application to elucidation of molecular recognition mechanisms

Research Project

Project/Area Number 24310158
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Living organism molecular science
Research InstitutionOsaka University

Principal Investigator

IWAI Shigenori  大阪大学, 基礎工学研究科, 教授 (10168544)

Co-Investigator(Renkei-kenkyūsha) SUGASAWA Kaoru  神戸大学, バイオシグナル研究センター, 教授 (70202124)
KOJIMA Chojiro  大阪大学, 蛋白質研究所, 准教授 (50333563)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
KeywordsDNA / 構造変化 / ジスルフィド結合 / HPLC / タンパク質 / 分子認識 / DNA / HPLC
Outline of Final Research Achievements

To detect the DNA helix bending by a chemical approach, duplexes containing 2-O-mercaptoalkyl-β-D-arabinofuranose on both sides of the major groove were synthesized, and the effects of the cisplatin adduct, the abasic site analog, and the (6-4) photoproduct on DNA structures were investigated. A disulfide bond was formed depending on the cisplatin adduct, and dynamic DNA bending was shown for the abasic site and the (6-4) photoproduct. Using the bent structure obtained by this method, the model proposed for the handover of damaged DNA from the UV-DDB protein to the XPC protein in the nucleotide excision repair was validated.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (3 results)

All 2015 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Analysis of structural flexibility of damaged DNA using thiol-tethered oligonucleotide duplexes2015

    • Author(s)
      Masashi Fujita, Shun Watanabe, Mariko Yoshizawa, Junpei Yamamoto, Shigenori Iwai
    • Journal Title

      PLoS ONE

      Volume: 10 Issue: 2 Pages: e0117798-e0117798

    • DOI

      10.1371/journal.pone.0117798

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Chemical and electrochemical analysis of dynamic structural change of UV-damaged DNA2013

    • Author(s)
      渡邉 駿
    • Organizer
      第40回国際核酸化学シンポジウム
    • Place of Presentation
      神奈川大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Flexibility of abasic site-containing DNA duplex as analyzed with thiol-tethered oligonucleotides2012

    • Author(s)
      藤田昌志
    • Organizer
      第39回国際核酸化学シンポジウム
    • Place of Presentation
      名古屋大学
    • Year and Date
      2012-11-15
    • Related Report
      2012 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi