Development of novel mosquito-control system using Bacillus thuringiensis Cry toxins
| Project/Area Number |
24380034
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Okayama University |
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Principal Investigator |
HAYAKAWA Tohru 岡山大学, 自然科学研究科, 助教 (30313555)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | Bacillus thuringiensis / Mosquitocidal toxins / Cry toxin / Scorpion toxin / Synergism / Bacillus tnuringiensis / Mosquito larvae / Cry4Aa toxin / Cry11Ba toxin / Cry11Aa toxin / Cryトキシン / アカイエカ / 人工遺伝子 / 害虫防除 / 微生物殺虫剤 / Cry4Aa / Cry4Ba / Cry11Aa / Cyt1Aa / アラニンスキャニング |
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| Outline of Final Research Achievements |
Mosquitos are vectors of important diseases like malaria and viral hemorrhagic fevers. Therefore, I tried to develop novel mosquito-control system using mosquitocidal toxin derived from soil bacterium, Bacillus thuringiensis. This system were supposed to be safe and are able to prevent, or at least delay the development of resistance-insects. In this study, I established the production systems for several mosquitocidal toxins (e.g. Cry11Ba, Cyt1Aa, AahIT, BjαIT) and analyzed their mosquitocidal mechanisms by several procedures (e.g. alanine scanning, binding assay). We also observed the synergism of mosquitocidal activity between Cry4Aa and Cry11Ba.
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Report
(4 results)
Research Products
(18 results)
