Transactivation potencies of peroxisome proliferator-activated receptor alpha by brominated flame retardants
Project/Area Number |
24380179
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Boundary agriculture
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Research Institution | Ehime University (2014) Shokei Junior College (2012-2013) |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
ARIZONO Koji 熊本県立大学, 環境共生学部, 教授 (70128148)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
|
Keywords | 残留性有機汚染物質 / 臭素系難燃剤 / 核内受容体 / PPARα / 環境汚染 / 生態系保全 / リスク評価 / 遺伝子 / 生態系 / PPARα |
Outline of Final Research Achievements |
The present study assessed the transactivation potencies of the Baikal seal (Pusa sibirica) peroxisome proliferator-activated receptor α (bsPPARα) by brominated flame retardants (BFRs) using an in vitro reporter gene assay. Among seven BFRs treated in a range of 2.4-50 microM concentration, three PBDEs, BDE47, 99 and 153, induced bsPPARα-mediated transcriptional activities in a dose-dependent manner. To compare the bsPPARα transactivation potencies by PBDEs, the present study estimated the perfluorooctanoic acid (PFOA) induction equivalency factors (IEFs). The order of IEFs for the PBDEs was as follows; BDE153 (IEF: 17) > BDE99 (12) > BDE47 (7.4) >> PFOA (1.0) >>> BDE100, BDE154, BDE183 and HBCDs (not activated). This is the first study in which the transactivation potencies of PPARα by PBDEs in aquatic mammals were clarified. Our study demonstrates that the PPARα-mediated response may be a useful biomarker to evaluate potential biological effects of PBDEs in wildlife.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Trophic magnification of polychlorinated biphenyls and polybrominated diphenyl ethers in an estuarine food web of the Ariake Sea, Japan2015
Author(s)
Kobayashi, J., Imuta, Y., Komorita, T., Yamada, K., Ishibashi, H., Ishihara, F., Nakashima, N., Sakai, J., Arizono, K., Koga, M.
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Journal Title
Chemosphere
Volume: 118
Pages: 201-206
DOI
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Peer Reviewed / Open Access
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[Journal Article] Identification and characterization of the androgen receptor from the American alligator, Alligator mississippiensis2015
Author(s)
Miyagawa, S., Yatsu, R., Kohno, S., Doheny, B.M., Ogino, Y., Ishibashi, H., Katsu, Y., Ohta, Y., Guillette, L.J.Jr., Iguchi, T.
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Journal Title
Endocrinology
Volume: in press
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Peer Reviewed / Open Access
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[Journal Article] Endocrine-disrupting potentials of equine estrogens equilin, equilenin, and their metabolites, in the medaka Oryzias latipes: in silico and DNA microarray studies2015
Author(s)
Uchida, M., Ishibashi, H., Yamamoto, R., Koyanagi, A., Kusano, T., Tominaga, N., Ishibashi, Y., Arizono, K.
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Journal Title
Journal of Applied Toxicology
Volume: in press
Issue: 9
Pages: 1040-1048
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Effects of lithium on growth, maturation, reproduction, and gene expression in the nematode Caenorhabditis elegans2015
Author(s)
Inokuchi, A., Yamamoto, R., Morita, F., Takumi, S., Matsusaki, H., Ishibashi, H., Tominaga, T., Arizono, K.
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Journal Title
Journal of Applied Toxicology
Volume: in press
Issue: 9
Pages: 999-1006
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Comparative study of the biological effects of antimicrobials, triclosan and trichlocarban, for C. elegans2014
Author(s)
Inokuchi, A., Nihira, M., Minakoshi, M., Yamamoto, R., Ishibashi, H., Tominaga, N., Arizono, K.
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Journal Title
Journal of Environment and Safety
Volume: 4
NAID
Related Report
Peer Reviewed
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[Journal Article] Effects of lithium on growth, maturation, reproduction, and gene expression in the nematode Caenorhabditis elegans2014
Author(s)
Inokuchi, A., Yamamoto, R., Morita, F., Takumi, S., Matsusaki, H., Ishibashi, H., Tominaga, T., Arizono, K.
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Journal Title
Journal of Applied Toxicology
Volume: 34
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Peer Reviewed
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[Journal Article] Quantitative analysis of the interaction of constitutive androstane receptor with chemicals and steroid receptor coactivator 1 using surface Plasmon resonance biosensor systems : A case study of the Baikal seal (Pusa sibirica) and mouse.2013
Author(s)
Dau, P.T., Sakai, H., Hirano. M., Ishibashi, H., Tanaka, Y., Kameda, K., Fujino, T., Kim, E,Y. and Iwata, H.
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Journal Title
Toxicol. Sci.
Volume: 131(1)
Issue: 1
Pages: 116-127
DOI
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Peer Reviewed
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[Journal Article] Study of dietary phytoestrogens and estrogenic activity in pet animal diets2013
Author(s)
Nakashima, S., Ji, H., Ishitobi, S., Kawazoe, S., Koga, M., Shinohara, R., Ishibashi, H., Tominaga, N., Arizono, K.
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Journal Title
Japanese Journal of Food Chemistry and Safety
Volume: 20
Pages: 22-30
NAID
Related Report
Peer Reviewed
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[Presentation] Gain of function mutations in two paralogous Androgen receptor (AR) genes of teleosts: Implications for evolution of AR gene function2015
Author(s)
Ogino, Y., Kuraku, S., Ishibashi, H., Miyakawa, H., Miyagawa, S., Yamada, G., Baker, M.E., Iguchi, T.
Organizer
48th Annual Meeting of the Japanese Society of Developmental Biologists
Place of Presentation
Tsukuba
Year and Date
2015-06-02 – 2015-06-05
Related Report
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[Presentation] Transcriptomic analysis of the liver of Japanese medaka (Oryzias latipes) exposed to Kanechlor-500, BDE47 or PFOS2013
Author(s)
Ishibashi, H., Uchida, M., Hirano, M., Kobayashi, J., Komorita, T., Nakamura, H., Kagami, Y., Kusano, T., Ishibashi, Y., Arizono, K.
Organizer
7th International Conference on Marine Pollution and Ecotoxicology
Place of Presentation
Hong Kong
Related Report
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