Development of a control method of protein function within cell using photodegradable nanoparticles and its medical applications

• Project/Area Number: 24390007
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: Kato Masaru 東京大学, 薬学研究科(研究院), 准教授 (30332943)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000); Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
• Keywords: ナノ粒子 / タンパク質 / 細胞 / 光 / 機能制御 / 病態モデル

Outline of Final Research Achievements

To examine the cellular reaction, we developed photodegradable nanoparticles that penetrate cellular membrane with encapsulated molecule and released the encapsulated molecule by light stimulus. We compared the cellular response after releasing the different amount of protein within cell using the nanoparticles. Our results indicated that the nanoparticles is a useful tool for the study of cellular reaction.

Research Products

• [Journal Article] Spatiotemporal activation of molecules within cells using silica nanoparticles responsive to blue-green light (2015)
  • Author(s): T. Amamoto, Y. Hirata, H. Takahashi, M. Kamiya, Y. Urano, T. Santa, M. Kato
  • Journal Title: J. Mater. Chem. B Volume: 3 Issue: 37 Pages: 7427-7433
  • DOI: 10.1039/c5tb01165e
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Surfactant-free aqueous preparation from a star polymer of size-controlled nanoparticles with encapsulated functional molecules (2015)
  • Author(s): Y. Shibata, T. Santa, M. Kato,
  • Journal Title: RSC Advances Volume: 5 Issue: 81 Pages: 65909-65912
  • DOI: 10.1039/c5ra12205h
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Comparison of the migration behavior of nanoparticles based on polyethylene glycol and silica using micellar electrokinetic chromatography (2015)
  • Author(s): M. Kato, M. Sasaki, Y. Ueyama, A. Koga, A. Sano, T. Higashi, T. Sanata
  • Journal Title: Journal of Separation Sciences Volume: 38 Issue: 3 Pages: 468-474
  • DOI: 10.1002/jssc.201401086
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Gene regulation by intracellular delivery and photodegradation of nanoparticles containing small interfering RNA (2014)
  • Author(s): S. Murayama, P. Kos, K. Miyata, K. Kataoka, E. Wagner, M. Kato
  • Journal Title: Macromolecular Bioscience Volume: 14 Issue: 5 Pages: 626-631
  • DOI: 10.1002/mabi.201300393
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A computer simulation study of the network structure of a hydrogel prepared from a tetra-armed star pre-polymer (2014)
  • Author(s): K. Takagi, S. Murayama, T. Sakai, M. Asai, T. Santa, M. Kato
  • Journal Title: Soft Matter Volume: 10 Issue: 20 Pages: 3553-3559
  • DOI: 10.1039/c3sm52908h
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reduction of Molecular Leaching from a Gel Matrix for the Precisely Controlled Release of Encapsulated Molecules by Light Stimulus (2014)
  • Author(s): T. Amamoto, T. Santa, M. Kato
  • Journal Title: Chemical and Pharmaceutical Bulletin Volume: 62 Issue: 7 Pages: 649-653
  • DOI: 10.1248/cpb.c14-00094
  • NAID: 130004053802
  • ISSN: 0009-2363, 1347-5223
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Development a spatiotemporal control method of molecular function using silica-based photodegradable nanoparticles that can be prepared at a mild condition (2014)
  • Author(s): Ishizuka, X.S. Liu, S. Murayama, T. Santa, M. Kato
  • Journal Title: Journal of Materials Chemistry B Volume: 2 Issue: 26 Pages: 4153-4158
  • DOI: 10.1039/c4tb00536h
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The Simple Preparation of Polyethylene Glycol-Based Soft Nanoparticles Containing Dual Imaging Probes. (2013)
  • Author(s): Murayama S, Jo JI, Shibata Y, Liang K, Santa T, Saga T, Aoki I, Kato M.
  • Journal Title: J Mater Chem B Mater Biol Med Volume: 1 Issue: 38 Pages: 4932-4938
  • DOI: 10.1039/c3tb20828a
  • Peer Reviewed
• [Journal Article] NanoPARCEL : a method for controlling cellular behavior with external light. (2012)
  • Author(s): Murayama, M., Su, B., Okabe, K., Kishimura, A., Osada, K., Miura, M., Funatsu, T., Kataoka, K., and Kato, M
  • Journal Title: Chem Comm. Volume: 48 Issue: 67 Pages: 8380-8382
  • DOI: 10.1039/c2cc32718j
  • Peer Reviewed
• [Journal Article] Delivery, stabilization, and spatiotemporal activation of cargo molecules in cells with positively charged nanoparticles (2012)
  • Author(s): Shuhei Murayama
  • Journal Title: Chem. Comm. Volume: 48 Issue: 93 Pages: 11461-11463
  • DOI: 10.1039/c2cc35567a
  • Peer Reviewed
• [Presentation] ナノマテリアルの分離分析法の開発 (2016)
  • Author(s): 加藤 大
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-03-27
• [Presentation] Development of nanoparticles and their analytical method for medical application (2016)
  • Author(s): Masaru Kato
  • Organizer: BK21Plus Symposium for Nanobio Materials and Advanced Analytical Techniques
  • Place of Presentation: 昌原大学（韓国）
  • Year and Date: 2016-01-28
  • Int'l Joint Research / Invited
• [Presentation] 経皮投与用DDSを目指した可視光応答性ナノ粒子の開発 (2015)
  • Author(s): 天本宇紀，三田智文，船津高志，加藤大
  • Organizer: 新アミノ酸分析研究会・第四回学術講演会
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-12-07
• [Presentation] 可視光応答性ナノ粒子を用いた細胞内物質の放出制御 (2015)
  • Author(s): 天本宇紀，平田智也，高橋光規，神谷真子，浦野泰照，三田智文，加藤 大
  • Organizer: 第35回キャピラリー電気泳動シンポジウム(SCE2015)
  • Place of Presentation: 岡山大学（岡山県岡山市）
  • Year and Date: 2015-11-05
• [Presentation] モノリス型カラムによるナノメディシンの分離 (2015)
  • Author(s): 加藤 大
  • Organizer: 日本分析化学会第64年会
  • Place of Presentation: 九州大学（福岡県福岡市西区）
  • Year and Date: 2015-09-10
• [Presentation] 経皮投与可能な抗体内包可視光分解性ナノ粒子の開発 (2015)
  • Author(s): 天本宇紀,三田智文,加藤 大
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸サンボーホール（兵庫県神戸市）
  • Year and Date: 2015-03-26
• [Presentation] Separation of nanoparticles using CE and HPLC (2014)
  • Author(s): Masaru Kato, Naoki Itoh, Minoru Sasaki
  • Organizer: 14th Asia-Pacific International Symposium on Microscale Separations and Analysis
  • Place of Presentation: 京都大学（京都府京都市）
  • Year and Date: 2014-12-09
• [Presentation] 可視光 で内包物を放出する光応答性ゲルの開発 (2014)
  • Author(s): 天本 宇紀、三田 智文、加藤 大
  • Organizer: 第27回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 帝京大学（東京都板橋区）
  • Year and Date: 2014-08-21
• [Presentation] 光分解性ナノ粒子を利用して規定量の生理活性物質を細胞内に放出した際の応答解析 (2014)
  • Author(s): 柴田悠圭、三田智文、加藤 大
  • Organizer: 次世代を担う若手のためのフィジカル・ファーマフォーラム
  • Place of Presentation: 箱根高原ホテル（神奈川県足柄下郡箱根町）
  • Year and Date: 2014-07-15
• [Presentation] タンパク質内包ナノ粒子を用いた細胞の機能制御 (2013)
  • Author(s): 加藤 大
  • Organizer: 第26回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 昭和大学（東京都品川区）
  • Year and Date: 2013-08-02
  • Invited
• [Presentation] タンパク質内包ナノ粒子を用いた細胞の機能制御 (2013)
  • Author(s): 加藤大
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜(神奈川県)(招待講演)
  • Year and Date: 2013-03-28
• [Presentation] Control of Protein Behaviors in Living Cell Using Protein Encapsulated Nanoparticle (2013)
  • Author(s): Masaru KATO
  • Organizer: Pittcon
  • Place of Presentation: フィラデルフィア, USA(招待講演)
  • Year and Date: 2013-03-20
• [Presentation] 光分解性ゲルを利用したタンパク質の機能制御 (2012)
  • Author(s): 加藤大
  • Organizer: 第3回ミニシンポジウム「ソフト界面と計測、センシング」
  • Place of Presentation: 東京医科歯科大学(東京都)(招待講演)
  • Year and Date: 2012-08-08
• [Remarks] 研究室のﾎｰﾑﾍﾟｰｼﾞ
  • URL: http://park.itc.u-tokyo.ac.jp/CNBI/kato/index.html
• [Remarks] 研究室のHP
  • URL: http://park.itc.u-tokyo.ac.jp/CNBI/kato/index.html
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/CNBI/kato/index.html