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Mechanism to generate lipid-based supramolecular structures

Research Project

Project/Area Number 24390045
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNagoya University

Principal Investigator

FUJIMOTO Toyoshi  名古屋大学, 医学(系)研究科(研究院), 教授 (50115929)

Co-Investigator(Kenkyū-buntansha) OHSAKI Yuki  名古屋大学, 大学院医学系研究科, 講師 (00378027)
SUZUKI Michitaka  名古屋大学, 大学院医学系研究科, 特任助教 (80456649)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords脂質ドメイン / 脂肪滴 / オートファゴソーム / 電子顕微鏡 / 膜脂質 / オートファジー / 生体膜 / ホスファチジルイノシトール3燐酸 / 急速凍結・凍結割断レプリカ標識法 / PKC / 急速凍結 / 凍結割断レプリカ
Outline of Final Research Achievements

We studied on lipid-based supramolecular structures, i.e., lipid droplet, autophagosome, and lipid domain. The results obtained are as follows: 1) In mice lacking a lipid droplet-resident protein, UBXD8, in liver, hepatocytes are deficient in lipoprotein secretion and exhibit an atypical type of steatosis; 2) Phosphatidylinositol 3-phosphate, a phospholipid indispensable for autophagy, takes drastically different distributions in autophagosomes between budding yeast and mammalian cells; 3) Distribution of phosphatidylcholine across the plasmalemmal lipid bilayer is different between budding yeast and mammalian cells.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (28 results)

All 2015 2014 2013 2012 Other

All Journal Article (13 results) (of which Peer Reviewed: 12 results,  Open Access: 1 results) Presentation (12 results) (of which Invited: 8 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Microscopy of membrane lipids: how precisely can we define their distribution?2015

    • Author(s)
      Takatori S and Fujimoto T
    • Journal Title

      Essays Biochem.

      Volume: 57 Pages: 81-91

    • DOI

      10.1042/bse0570081

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Asymmetrical distribution of choline phospholipids revealed by click chemistry and freeze-fracture electron microscopy.2014

    • Author(s)
      Iyoshi S, Cheng J, Tatematsu T, Takatori S, Taki M, Yamamoto Y, Salic A and Fujimoto T
    • Journal Title

      ACS Chem. Biol.

      Volume: 9(10) Issue: 10 Pages: 2217-2222

    • DOI

      10.1021/cb500558n

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 生体膜脂質の局在可視化法2014

    • Author(s)
      高鳥翔、藤本豊士
    • Journal Title

      生化学

      Volume: 86 Pages: 5-17

    • NAID

      40020011044

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Inhibition of NPC1L1 by ezetimibe activates autophagy in human hepatocyte and reduces mutant α1-antitrypsin Z deposition.2014

    • Author(s)
      Yamamura T, Ohsaki Y, Suzuki M, Shinohara Y, Tatematsu T, Cheng J, Okada M, Ohmiya N, Hirooka Y, Goto H, Fujimoto T
    • Journal Title

      Hepatology

      Volume: 59 Issue: 4 Pages: 1591-1599

    • DOI

      10.1002/hep.26930

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Integrated omics study delineates the dynamics of lipid droplets in the Rhodococcus opacus PD630.2014

    • Author(s)
      Chen Y, Ding Y, Yang L, Yu J, Liu G, Wang X, Zhang S, Yu D, Song L, Zhang H, Zhang C, Huo L, Huo C, Wang Y, Du Y, Zhang H, Zhang P, Na H, Xu S, Zhu Y, Xie Z, He T, Zhang Y, Wang G, Fan Z, Yang F, Liu H, Wang X, Zhang X, Zhang MQ, Li Y, Steinbüchel A, Fujimoto T, Cichello S, Yu J, Liu P.
    • Journal Title

      Nucleic Acids Res

      Volume: 42 Issue: 2 Pages: 1052-1064

    • DOI

      10.1093/nar/gkt932

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Yeast and mammalian autophagosomes exhibit distinct phosphatidylinositol 3-phosphate asymmetries2014

    • Author(s)
      Cheng J, Fujita A, Yamamoto H, Tatematsu T, Kakuta S, Obara K, Ohsumi Y, Fujimoto T.
    • Journal Title

      Nat. Commun.

      Volume: 5 Issue: 1 Pages: 3207-3207

    • DOI

      10.1038/ncomms4207

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Open questions in lipid droplet biology2014

    • Author(s)
      Ohsaki Y, Suzuki M, Fujimoto T.
    • Journal Title

      Chem Biol

      Volume: 21 Issue: 1 Pages: 86-96

    • DOI

      10.1016/j.chembiol.2013.08.009

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microscopic methods to observe the distribution of lipids in the cellular membrane2014

    • Author(s)
      Sho Takatori, Rob Mesman, Toyoshi Fujimoto
    • Journal Title

      Biochemistry

      Volume: Vol.53(4) Issue: 4 Pages: 639-653

    • DOI

      10.1021/bi401598v

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein kinase Cη is targeted to lipid droplets.2013

    • Author(s)
      Suzuki Michitaka
    • Journal Title

      Histochem Cell Biol

      Volume: 139 Issue: 4 Pages: 505-511

    • DOI

      10.1007/s00418-013-1083-z

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of ADP-ribosylation suppresses aberrant accumulation of lipidated apolipoprotein B in the endoplasmic reticulum.2013

    • Author(s)
      Ohsaki Y, Cheng J, Yamairi K, Pan X, Hussain MM, Fujimoto T.
    • Journal Title

      FEBS Lett

      Volume: 587 Issue: 22 Pages: 3696-3702

    • DOI

      10.1016/j.febslet.2013.09.036

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Derlin-1 and UBXD8 are engaged in dislocation and degradation of lipidated ApoB-100 at lipid droplets2012

    • Author(s)
      Suzuki M, Otsuka T, Ohsaki Y, Cheng J, Taniguchi T, Hashimoto H, Taniguchi H, Fujimoto T
    • Journal Title

      Mol Biol Cell

      Volume: (印刷中)(in press) Issue: 5 Pages: 800-810

    • DOI

      10.1091/mbc.e11-11-0950

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Translation inhibitors induce formation of cholesterol ester-rich lipid droplets2012

    • Author(s)
      Suzuki M, Ohsaki Y, Tatematsu T. Shinohara Y, Maeda T, Cheng J, and Fujimoto T.
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 8 Pages: e42379-e42379

    • DOI

      10.1371/journal.pone.0042379

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A method for efficient observation of intracellular membranes of monolayer culture cells by quick-freeze and freeze-fracture electron microscopy.2012

    • Author(s)
      Akikazu Fujita, Toyoshi Fujimoto, Nami Ozato-Sakurai, Hitomi Suzuki
    • Journal Title

      J Electron Microsc (Tokyo)

      Volume: 61 Issue: 6 Pages: 441-446

    • DOI

      10.1093/jmicro/dfs063

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Nanoscale analysis of membrane domains2014

    • Author(s)
      Fujimoto T
    • Organizer
      Freiburg-Adelaide-Nagoya Retreat
    • Place of Presentation
      Adelaide, Australia
    • Year and Date
      2014-09-24
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Electron microscopy for the nanoscale analysis of membrane lipid distribution2014

    • Author(s)
      Fujimoto T
    • Organizer
      SciLifeLab, The Svedberg seminar series
    • Place of Presentation
      Uppsala, Sweden
    • Year and Date
      2014-09-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Distribution of membrane lipids observed by quick-freezing/freeze-fracture replica labeling electron microscopy2014

    • Author(s)
      Fujimoto T
    • Organizer
      The 18th International Microscopy Congress
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2014-09-06 – 2014-09-12
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 膜脂質の微細局在を見るための方法について2013

    • Author(s)
      藤本豊士
    • Organizer
      第8回スフィンゴテラピィ研究会
    • Place of Presentation
      山中温泉河鹿荘ロイヤルホテル(加賀市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Nanoscale distribution of membrane lipids observed by electron microscopy2013

    • Author(s)
      Toyoshi Fujimoto
    • Organizer
      47th Symposium of The Polish Society for Histochemistry and Cytochemistry
    • Place of Presentation
      Olsztyn, Poland
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 細胞質と核における脂肪滴の生理的意義2013

    • Author(s)
      大﨑雄樹、藤本豊士
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Analysis of Membrane Lipids by Electron Microscopy2013

    • Author(s)
      Toyoshi Fujimoto
    • Organizer
      XXIII International Symposium on Morphological Sciences
    • Place of Presentation
      朱鷺メッセ新潟コンベンションセンター(新潟市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 脂肪滴の新たな機能と動態2013

    • Author(s)
      藤本豊士
    • Organizer
      細胞内ロジスティクス・シンポジウム
    • Place of Presentation
      淡路夢舞台国際会議場(淡路市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Nanoscale observation of membrane lipids by electron microscopy2012

    • Author(s)
      Fujimoto T
    • Organizer
      Gordon Research Conference "Glycolipid & Sphingolipid Biology"
    • Place of Presentation
      Lucca, Italy
    • Related Report
      2012 Annual Research Report
  • [Presentation] Derlin-1 and UBXD8 are related to dislocation and degradation of lipidated ApoB-100 at the ER-LD juncture2012

    • Author(s)
      Suzuki M, Fujimoto T
    • Organizer
      The 14th International Congress of Histochemistry and Cytochemistry
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 膜脂質のナノ局在を電子顕微鏡で見る2012

    • Author(s)
      藤本豊士
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      ロイトン札幌(札幌)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Distribution of phospholipids in yeasts and mammals2012

    • Author(s)
      Fujimoto T
    • Organizer
      RIKEN Symposium "Evolution of lipids, lipids in evolution"
    • Place of Presentation
      理化学研究所(和光)
    • Related Report
      2012 Annual Research Report
  • [Book] Encyclopedia of Biophysics2012

    • Author(s)
      Fujimoto T, Fukazawa Y.
    • Publisher
      Springer-Verlag
    • Related Report
      2012 Annual Research Report
  • [Remarks] 名古屋大学大学院医学系研究科・分子細胞学分野ホームページ

    • URL

      http://www.med.nagoya-u.ac.jp/cel-bio/index-j.html

    • Related Report
      2014 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.nagoya-u.ac.jp/cel-bio/index.htm

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

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