Cistromic analysis of EMT regulators in breast cancer cells

• Project/Area Number: 24501311
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: The University of Tokyo
• Principal Investigator: KOINUMA Daizo 東京大学, 医学(系)研究科(研究院), 准教授 (80375071)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: FAIRE-seq / シグナル伝達 / EMT / 乳がん / TGF-beta / ZEB1 / アポトーシス / ゲノム / 癌 / 転写因子 / lncRNA / lincRNA / FAIRE / RNA-seq / オープンクロマチン / TGF-β

Outline of Final Research Achievements

Transcriptomic analysis was performed by RNA-seq during EMT of mammary epithelial cells. We found a list of differentially expressed non-coding RNAs during this process. We identified lncRNA-Smad7 as an anti-apoptotic non-coding RNA. A FAIRE-seq dataset was also obtained and we observed promising data showing the dynamic changes in the open chromatin regions during EMT.

Research Products

• [Journal Article] The Arkadia-ESRP2 axis suppresses tumor progression: Analyses in clear cell renal cell carcinoma (2015)
  • Author(s): Mizutani A, Koinuma D, Seimiya H and Miyazono K.
  • Journal Title: Oncogene Volume: 印刷中
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transforming growth factor-β-induced lncRNA-Smad7 inhibits apoptosis of mouse breast cancer JygMC(A) cells (2014)
  • Author(s): Arase M, Horiguchi K, Ehata S, Morikawa M, Tsutsumi S, Aburatani H, Miyazono K, Koinuma D.
  • Journal Title: Cancer Science Volume: 105 Issue: 8 Pages: 974
  • DOI: 10.1111/cas.12454
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Identification of Smad regulatory factors through integrated analysis of ChIP-seq data (2015)
  • Author(s): Koinuma D.
  • Organizer: Joint International Symposium on TGF-β Family and Cancer : Signal Network in Tumor Microenvironment
  • Place of Presentation: つくば
  • Year and Date: 2015-01-12 – 2015-01-13
  • Invited
• [Presentation] FAIRE-seq analysis of TGF-β-induced EMT in mouse mammary gland epithelial cells (2015)
  • Author(s): Arase M, Koinuma D, Isogaya K, Mizutani A, Tsutsumi S, Aburatani H, Miyazono K.
  • Organizer: Joint International Symposium on TGF-β Family and Cancer : Signal Network in Tumor Microenvironment
  • Place of Presentation: つくば
  • Year and Date: 2015-01-12 – 2015-01-13
• [Presentation] Arkadiaによるスプライシング制御因子ESRP2の機能制御 (2014)
  • Author(s): 水谷アンナ、鯉沼代造、宮園浩平
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] TGF-β誘導性long non-coding RNA-Smad7はマウス乳がん細胞のアポトーシスを抑制する (2014)
  • Author(s): 荒瀬麻友、堀口華奈、江幡正悟、森川真大、堤 修一、油谷浩幸、宮園浩平、鯉沼代造
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Regulation of EMT and related phenotypes by TGF-β in cancer cells (2014)
  • Author(s): Koinuma D, Isogaya K, Sakurai T, Kawasaki N, Ehata S, Tsutsumi S, Saito A, Miyazawa K, Aburatani H, Miyazono K.
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
  • Invited
• [Presentation] TGF-β誘導性long non-coding RNA-Smad7はマウス乳がん細胞のアポトーシスを抑制する (2014)
  • Author(s): 荒瀬麻友、江幡正悟、堤 修一、油谷浩幸、宮園浩平、鯉沼代造
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] E3リガーゼArkadiaによるスプライシング制御因子ESRP2の機能制御 (2014)
  • Author(s): 水谷アンナ、鯉沼代造、宮園浩平
  • Organizer: 平成26年度 がん若手研究者ワークショップ
  • Place of Presentation: 茅野
  • Year and Date: 2014-09-03 – 2014-09-06
• [Presentation] Transforming growth factor-β-inducedlong non-coding RNA-Smad7 inhibits apoptosis of mouse breast cancer cells (2014)
  • Author(s): 荒瀬麻友、堀口華奈、江幡正悟、森川真大、堤 修一、油谷浩幸、宮園浩平、鯉沼代造
  • Organizer: 第14回東京大学生命科学ネットワークシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2014-04-26
• [Presentation] Transforming growth factor-β-induced long non-coding RNA-Smad7 inhibits apoptosis of mouse breast cancer cells (2014)
  • Author(s): 荒瀬 麻友、堀口 華奈、江幡 正悟、森川 真大、堤 修一、油谷 浩幸、宮園 浩平、鯉沼 代造
  • Organizer: 第14回東京大学生命科学シンポジウム
  • Place of Presentation: 東京都文京区