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Potential of microbial technology for waste-to-energy process

Research Project

Project/Area Number 24510101
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental technology/Environmental materials
Research InstitutionYamaguchi University

Principal Investigator

Fujii Katsuhiko  山口大学, 創成科学研究科, 准教授 (30333660)

Project Period (FY) 2012-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Keywordsセルロース / バイオマス / セルロースバイオマス / 下水バイオマス / 土壌微生物 / 微生物 / セルラーゼ / キシラナーゼ
Outline of Final Research Achievements

In this study, combinations of cellulolytic microbes and fermentation microbes were assayed to find one which can produce biofuel directly from cellulosic biomass. In consequence, addition of a culture supernatant of Gilvimarinus japonicus to yeast was found to produce ethanol from seaweed biomass. Addition of a culture supernatant of Trametes versicolor to hydrogen-producing bacterial flora was found to produce hydrogen from several lignocellulosic biomass. Our results strongly suggest that a suitable combination of cellulolytic strains and fermentation strains can transform cellulosic biomass directly to biofuel.

Report

(6 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (19 results)

All 2017 2016 2015 2014 2013 2012

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Acknowledgement Compliant: 4 results) Presentation (12 results)

  • [Journal Article] Digested sludge-degrading and hydrogen-producing bacterial floras and their potential for biohydrogen production.2017

    • Author(s)
      Kuribayashi, K., Kobayashi, Y., Yokoyama, K., Fujii, K.
    • Journal Title

      International Biodeterioration and Biodegradation

      Volume: 120 Pages: 58-65

    • DOI

      10.1016/j.ibiod.2017.02.009

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Gilvimarinus japonicus sp. nov., a cellulolytic and agarolytic marine bacterium isolated from coastal debris.2016

    • Author(s)
      Kouzui, H., Tokikawa, K., Satomi, M., Negoro, T., Shimabukuro, K., Fujii, K.
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 66 Issue: 6 Pages: 5417-5423

    • DOI

      10.1099/ijs.0.64130-0

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A method for isolation of soil microbial DNA that is suitable for analysis of microbial cellulase genes.2016

    • Author(s)
      Yoshiki Kobayashi, Erika Hayashida, Kazuhira Yokoyama & Katsuhiko Fujii
    • Journal Title

      Separation Science and Technology

      Volume: 51(6) Pages: 1053-1062

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Possible practical utility of an enzyme cocktail produced by sludge-degrading microbes for methane and hydrogen production from digested sludge.2016

    • Author(s)
      Sato, H., Kuribayashi, K., Fujii, K.
    • Journal Title

      New Biotechnology

      Volume: 33(1) Pages: 1-6

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Streptomyces abietis sp. nov., a cellulolytic bacterium isolated from the soil of a pine forest in Hokkaido, Japan.2013

    • Author(s)
      Fujii, K., Satomi, M., Fukui, Y., Matsunobu, S., Morifuku, Y., Enokida, Y.
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 63 Issue: Pt_12 Pages: 4754-4759

    • DOI

      10.1099/ijs.0.053025-0

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Isolation of digested sludge-assimilating fungal strains and their potential applications.2013

    • Author(s)
      Fujii, K., Kai, Y., Matsunobu, S., Sato, H., Mikami, A.
    • Journal Title

      Journal of Applied Microbiology

      Volume: 115 Issue: 3 Pages: 718-726

    • DOI

      10.1111/jam.12266

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of aerobic microorganisms with cellulolytic activity in the gut of endogeic earthworms2012

    • Author(s)
      Fujii, K., Ikeda, K., and Yoshida, S.
    • Journal Title

      INTERNATIONAL MICROBIOLOGY

      Volume: 15 Pages: 121-130

    • NAID

      120005246368

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 新奇セルロース分解海洋細菌Gilvimarinus japonicusの特性と海藻分解能の研究2017

    • Author(s)
      江水 秀之、時川 鴻希、里見 正隆、島袋 勝弥、藤井 克彦
    • Organizer
      日本農芸化学会 2017年大会
    • Place of Presentation
      京都女子大学(京都府京都市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 段階希釈を用いたセルロース分解微生物の集積培養2017

    • Author(s)
      織田 康太郎、横山 和平、藤井 克彦
    • Organizer
      日本農芸化学会 2017年大会
    • Place of Presentation
      京都女子大学(京都府京都市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 海洋からのセルロース分解微生物の分離2016

    • Author(s)
      江水 秀之、時川 鴻希、藤井 克彦
    • Organizer
      日本農芸化学会2016年大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] セルロースを混合した土壌における微生物生態系の変遷2016

    • Author(s)
      小林 慶紀、林田 英里香、横山 和平、藤井 克彦
    • Organizer
      日本農芸化学会2016年大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] 汚泥分解微生物の生化学的解析2016

    • Author(s)
      佐藤颯音、藤井克彦
    • Organizer
      日本農芸化学会2016年大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Potential Application of Digested Sludge-Degrading Microbes to Biogas Production2015

    • Author(s)
      Fuijii, K.
    • Organizer
      nternational Bioenergy (Shanghai) Exhibition and Asian Bioenergy Conference 2015
    • Place of Presentation
      上海Everbright会議場(中国・上海)
    • Year and Date
      2015-10-21
    • Related Report
      2015 Research-status Report
  • [Presentation] 新規酵素の探索を目指した、土壌DNA抽出法の検討2015

    • Author(s)
      小林慶紀、藤井克彦、横山和平
    • Organizer
      日本農芸化学会2015年大会
    • Place of Presentation
      岡山大学(岡山市)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] セルロース分解能を持つ新奇Streptomyces 属細菌( S abietis)の分離2014

    • Author(s)
      藤井 克彦、里見 正隆、福井 洋平、松延 駿、森福 洋二、榎田 裕哉
    • Organizer
      日本農芸化学会
    • Place of Presentation
      明治大学(川崎市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 3. Isolation and characterization of "digested sludge"-assimilating microbes.2013

    • Author(s)
      Fujii K, Matsunobu S, Kai T.
    • Organizer
      FEMS 2013
    • Place of Presentation
      ライプチヒ(ドイツ)
    • Related Report
      2013 Research-status Report
  • [Presentation] 消化汚泥分解微生物の探索2013

    • Author(s)
      藤井克彦, 甲斐隆泰, 松延駿, 三上綾香
    • Organizer
      環境バイオテクノロジー学会
    • Place of Presentation
      北九州国際会議場(北九州市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 消化汚泥分解微生物の分離と解析2013

    • Author(s)
      藤井克彦、佐藤颯音、甲斐隆泰
    • Organizer
      廃棄物資源循環学会
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2013 Research-status Report
  • [Presentation] Cellulolytic microbes isolated from the earthworm gut.2012

    • Author(s)
      Fujii, K., Ikeda, K., and Yoshida, S.
    • Organizer
      14th International Symposium for Microbial Ecology-ISME
    • Place of Presentation
      Copenhagen, Denmark
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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