Evaluation of DNA degradation Using Microwave dielectric Absorption Spectroscopy and Its Application to Dosimetry

• Project/Area Number: 24561039
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nuclear engineering
• Research Institution: University of Fukui
• Principal Investigator: IZUMI Yoshinobu 福井大学, 附属国際原子力工学研究所, 教授 (60252582)
• Co-Investigator(Kenkyū-buntansha): MATUO Youichirou 福井大学, 附属国際原子力工学研究所, 特命助教 (90568883) ; YAMANO Naoki 福井大学, 附属国際原子力工学研究所, 特命教授 (10397044) ; SUNAGAWA Takeyoshi 福井工業大学, 工学部, 教授 (60329456) ; KOJIMA Takao 大阪府立大学, 地域連携研究機構放射線研究センター, 助教 (70360047)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 線量評価 / 被曝線量 / マイクロ波 / DNA / 空洞共振器 / L-バンド / S-バンド / 2本鎖切断 / Lバンド / Sバンド / マイクロ波誘電吸収

Outline of Final Research Achievements

By using microwave technique, we have been succeeded in detect a change in conformation of aqueous solution containing DNA. The main chain scission of plasmid DNA could be recognized by analysing characteristic absorption of microwave. Moreover, accuracy was improved by controlling temperature, humidity etc.

Research Products

• [Presentation] マイクロ波誘電吸収法を用いたDNA損傷評価 (2） (2015)
  • Author(s): 平山誠、松尾 陽一郎、砂川武義、泉佳伸
  • Organizer: 日本原子力学会2015年春の年会
  • Place of Presentation: 茨城県
  • Year and Date: 2015-03-20 – 2015-03-22
• [Presentation] 新たな線量計開発のためのマイクロ波誘電吸収システムの構築 (2014)
  • Author(s): 平山誠、松尾陽一郎、砂川武義、泉佳伸
  • Organizer: 平成26年度応用物理学会 北陸・信越支 部学術講演会
  • Place of Presentation: 富山県
  • Year and Date: 2014-11-07 – 2014-11-08
• [Presentation] Development of Microwave Dielectric Absorption Spectroscopyfor the Application to Dosimetry Using Plasmid DNA (2014)
  • Author(s): Yoshinobu IZUMI, Youichirou MATUO, and Takeyoshi SUNAGAWA
  • Organizer: AOCRP-4
  • Place of Presentation: クアラルンプル、マレーシア
• [Presentation] An New Idea for dosimetry by using DNAs and Development of Microwave Dielectric Absorption Spectroscopy System (2013)
  • Author(s): Yoshinobu IZUMI, Youichirou MATUO, Shota OKADA, Takao KOJIMA and Takeyoshi SUNAGAWA
  • Organizer: ISORD-7
  • Place of Presentation: 三亜、中国
• [Presentation] マイクロ波誘電吸収法を用いたDNAの評価と 線量計応用のための基礎的検討 (2013)
  • Author(s): 岡田翔太、松尾陽一郎、河田拓也、砂川武義、小嶋崇夫、泉佳伸
  • Organizer: 日本原子力学会 2013年春の年会
  • Place of Presentation: 大阪府
• [Presentation] 生体物質を用いた線量計に関する研究 (2012)
  • Author(s): 河田拓也、岡田翔太、砂川武義、小嶋祟夫、松尾陽一郎、泉佳伸
  • Organizer: 日本放射線安全管理学会第11回学術大会
  • Place of Presentation: 大阪府
• [Presentation] THE DETECTION OF THE DNA STRUCTURAL CHANGE USING THE MICROWAVE DIELECTRIC ABSORPTION SPECTROMETRY AND SYSTEM CONSTRUCTION AIMING AT DOSIMETRY (2012)
  • Author(s): Yoshinobu IZUMI, Takeyoshi SUNAGAWA, Youichirou MATUO, Takuya KAWADA and Takao KOJIMA
  • Organizer: 12th International Conference on Radiation Shielding (ICRS-12)
  • Place of Presentation: 奈良県