Evaluation of radiotoxicity after iodine-131 therapy using DNA damage in lymphocytes
| Project/Area Number |
24591794
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IWABUCHI Kuniyoshi 金沢医科大学, 医学部, 教授 (10232696)
DOAI Mariko 金沢医科大学, 医学部, 助教 (40515673)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 甲状腺癌 / 放射線障害 / リンパ球 / γ-H2AX / 放射線組織障害 / DNA損傷 / 放射性ヨード治療 |
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| Outline of Final Research Achievements |
The purpose of our study was to evaluate the degree of cytological radiation damage to lymphocytes after I-131 therapy using γ-H2AX foci immunodetection. Methods: This study focused on 15 patients who underwent I-131 therapy. Venous blood samples were collected from each patient before therapy and 4 days thereafter. Lymphocytes were isolated and subjected to γ-H2AX immunofluorescence staining. Results: The number of foci per lymphocyte nucleus was 0.41 ± 0.51 before and 6.19 ± 1.80 after radioiodine therapy, and this difference was statistically significant. Absorbed doses estimated were 0.77± 0.31Gy. Conclusion: Our study suggests that γ-H2AX foci immunodetection is useful as a biomarker for the evaluation of the radiotoxicity after I-131 therapy. γ-H2AX foci immunodetection in lymphocytes may detect radiation-induced DNA damage associated with I-131 therapy of thyroid cancer.
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Report
(5 results)
Research Products
(5 results)
